Panel demonstrating antibody specificity for SIRT1-7.

<p>For each sirtuin antibody a negative control (antibody diluent only) is illustrated, along with a tissue core stained with the antibody. Antibody specificity was validated via the use of blocking peptide to compete with tissue staining, or IHC on formalin-fixed paraffin embedded cell pellets treated with resveratrol.</p

Chemotherapy improves outcome in patients with low SIRT3 tumour levels.

<p>Patients with low SIRT3 expression who received chemotherapy had (a) a longer time to recurrence (20 vs 11.8 months, p = 0.057) and (b) a longer time to death (27.9 vs 14.6 months, p = 0.032) than those who did not receive chemotherapy. Conversely, patients who expressed high levels of SIRT3 and were treated with chemotherapy had (a) a shorter time to relapse (14.3vs 30.7 months, p = 0.159) and (b) a shorter time to death (18.3 vs 34.7 months, p = 0.148) than those who did not receive chemotherapy, though this did not reach statistical significance. </p

SIRT7 may act as a prognostic factor for patients with pancreatic cancer.

<p>Survival analysis of the entire cohort did not reveal any significant differences in (a) disease-free survival or (b) disease-specific survival time between patients whose tumours had low or high SIRT7 nuclear expression. However, it was apparent from the survival plots that there was a division of the low/high SIRT7 expression lines at approximately 12–14 months. Analysis was therefore performed on the subset of patients that survived longer than 12 months (n = 44). Patients with low SIRT7 nuclear expression exhibited (c) a shorter disease-free survival time (p = 0.028, 20.1 vs 38 months) and (d) a decrease in disease-specific time (p = 0.026, 26.9 vs 43.1 months) than patients whose tumour expressed high SIRT7 levels.</p

SIRT3 expression levels are associated with outcome in patients not receiving chemotherapy.

<p>Kaplan-Meier survival analyses, stratified by chemotherapy status, were performed. Patients who did not receive chemotherapy and expressed low levels of cytoplasmic SIRT3 in their tumours were more likely to (a) relapse quicker (p = 0.05 11.8 vs 30.7 months) and (b) die earlier (p = 0.014, 14.6 vs 34.7 months) than patients who expressed high levels of SIRT3. Conversely there was no significant difference in outcome as measured by (c) time to recurrence or (d) time to death in terms of SIRT3 expression in patients who received chemotherapy.</p

Sirtuin Histoscores.

<p>Table showing the median histoscores for each of the sirtuins in the PDAC tissue cohorts.</p><p>Sirtuin Histoscores.</p

Representative figures of SIRT1-7 immunohistochemistry on pancreatic tumour tissue.

<p>Examples of pancreatic tumours expressing both low and high levels of SIRT1-7 are shown.</p