Isolement, caracterisation et importance technologique de deux glutenines de faible poids moleculaire chez le ble dur

SIGLECNRS T Bordereau / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc

Détection de transgènes dans les aliments issus de plantes transgéniques

National audienc

Organismes génétiquement modifiés, détection et étiquetage des produits qui en sont issus

National audienc

La PCR quantitative en temps réel : application à la quantification des OGM

24 ref.National audienc

Detection and discrimination of cereal and leguminous species in chestnut flour by duplex PCR

Equipe DGB, ex-UMR PIAInternational audienceChestnut and chestnut-derived products are quite expensive and thus a possible target for fraudulent labeling. To obtain a French Label of Origin, chestnut producers need to certify the purity of their products. Chestnut-derived products are consumed by people who may suffer from celiac disease or are allergic to cereals. For these reasons, we developed a qualitative PCR-based method to detect cereal and leguminous species in chestnut flour. The presence of common wheat and barley was determined by amplifying part of the puroindoline-a gene, the presence of rye by amplifying part of the secaloindoline-a gene, the presence of durum wheat, rice, maize and chickpea by amplifying part of lipid transfer protein genes, the presence of oat by amplifying part of a thionin gene, the presence of kidney bean by amplifying part of a late embryogenesis- abundant protein, the presence of soybean by amplifying part of a lectin gene and the presence of fava bean by amplifying part of a nodulin gene. A chestnut thaumatine gene was used as control. PCR conditions were optimized to detect 1% of adulteration. Duplex PCR and specially designed sets of primers that allow amplification of several related species limited the number and cost of analyses required. Interpretation of the results is facilitated by using a decision tre

Comparative expression of five Lea genes during wheat seed development and in response to abiotic stresses by real-time quantitative RT-PCR

61 ref.International audienceGene expression profiles of group 2 (dehydrins) and group 4 Late embryogenesis abundant (Lea) genes in developing seeds of Triticum durum and T. aestivum and in coleoptiles and coleorhizae of T. durum seedlings were monitored by real-time quantitative RT-PCR. The five genes exhibited clear differences in their accumulation pattern in wheat seed and in response to dehydration, low temperature, salinity and ABA. Td29b, Td16 and Td27e gene transcripts accumulate late in embryogenesis as expected for Lea genes, Td11 gene transcripts were present throughout seed development whereas no Td25a gene transcripts were detected in seeds. Drastic changes in the relative levels of Td29b, Td16, Td27e and Td11 transcripts occurred at the shift between the cell expansion and desiccation phases. All genes except the Td11 gene are more highly induced by dehydration in coleorhizae than in coleoptiles. In contrast, response to low temperature, salinity or ABA is higher in coleoptiles than in coleorhizae. Depending on both the gene and on the type of stress, a wide range of induction levels (8- to 100,000-fold) was observed

Quantification of common wheat adulteration of durum wheat pasta using real-time quantitative polymerase chain reaction (PCR)

International audienc

Puroindoline genes are highly conserved in diploid ancestor wheats and related species but absent in tetraploid Triticum species

57 ref.International audienc

Wheat non-specific lipid transfer protein genes display a complex pattern of expression in developing seeds

61 ref.International audienc

Monitoring of the early molecular resistance responses of coffee (Coffea arabica L.) to the rust fungus (Hemileia vastatrix) using real-time quantitative RT-PCR

UMR DAP équipe DGBInternational audienceMolecular resistance responses of coffee (Coffea arabica L.) to the orange rust fungus Hemileia vastatrix were monitored by real-time quantitative RT-PCR analysis of gene expression. Significant activation of coffee genes by fungal infection could be observed around 12–16 h post inoculation (hpi) in the incompatible interaction. Microscopic observations indicated that, at this time, only a limited number of fungal germlings already differentiated a penetration hypha through the stomata. Activation of the CaWRKY1 gene, putatively encoding a WRKY transcription factor also occurred in the compatible interaction, but was delayed to 24 hpi. In contrast, activation of the CaR111 gene encoding a protein of unknown function only occurred in the incompatible interaction. The CaNDR1 gene, an homolog of the Arabidopsis non-race specific disease resistance (ndr1) gene was only poorly induced by fungal infection. Wounding and salicylic acid treatment markedly activated CaWRKY1, CaNDR1 and CaR111 gene expression. These results showed that specific transcriptional responses of coffee were detected before penetration of H. vastatrix into the leaf had occurred, and suggest a possible role of activated genes in the molecular resistance responses of coffee to the rust fungu