Predicted Direct Relationships Among miRNA and Hemostatic or Adhesive Protein Targets.

<p>In response to LPS stimulation, previous studies have shown increased expression of miRNAs and interaction with target proteins. The relationships depicted are generated from Ingenuity Pathway Analyses (IPA, QIAGEN Redwood city, <a href="http://www.quiagen.com/ingenuity" target="_blank">www.quiagen.com/ingenuity</a>). Symbol definitions are included in the figure inset.</p

Changes in hemostatic and adhesion proteins in plasma or serum over a 48 hour period.

<p>Mean protein concentrations and standard deviation were determined in both control (●) and LPS (o) for plasma PAI-1 (A), plasma D-Dimer (B), serum sICAM-1 (C), serum sE-selectin (D), and plasma fibrinogen (F) for five animals at each time point. * indicates a significant (P<0.05) difference between the control and LPS-treated groups</p

Hemogram cell counts over a 48 hour period.

<p>Individual cell counts from both control (●) and LPS (o) for neutrophils (A), monocytes (B), lymphocytes (C), platelets (D), red blood cells (E), and reticulocytes (F). Presented are means and standard deviation for five animals at each time point. * indicates a significant (P<0.05) difference between the control and LPS-treated groups.</p

miRNA Changes in Serum from LPS-Treated Rats.

<p>miRNA Changes in Serum from LPS-Treated Rats.</p

Clinical observations in LPS-treated animals.

<p>Clinical observations in LPS-treated animals.</p

Histopathology, hematoxylin and eosin-stained sections.

<p>(A) Neutrophil sequestration (arrows) within pulmonary alveolar capillaries typical of 5/5 rats at hours 4, 8, 24, and 48 post-intraperitoneal LPS injection. (B) Normal pulmonary alveolar capillaries at 48 hours post-intraperitoneal saline injection. (C) Neutrophil sequestration (arrows) within hepatic sinusoids typical of 5/5 rats at hour 4 and 8 post-intraperitoneal LPS injection. (D) Normal hepatic sinusoids at 8 hours post-intraperitoneal saline injection. 400 X original magnification A, B, C, and D. (E) Single cell necrosis of lymphocytes (arrows) within splenic lymphoid follicles typical of 5/5 rats at hours 4 and 8 post-intraperitoneal LPS injection. (F) Normal spleen at 8 hours post-intraperitoneal saline injection. (G) Increased megakaryocytes (arrows) within spleen typical of 5/5 rats at hour 48 post-intraperitoneal LPS injection. 200 X original magnification E, F, and G. (H) Leukocyte accumulation in subepicardial myocardium of the right atrium typical of 5/5 rats at hour 48 post-intraperitoneal LPS injection. Inset 400X original magnification. (I) Normal right atrium at 48 hours post-intraperitoneal saline injection. 40 X original magnification H, I.</p

Experimental design and time-points analyzed.

<p>Experimental design and time-points analyzed.</p

Extracellular vesicle NTA counts.

<p>Population size distributions of extracellular vesicles in rat plasma. A. Fluorescence histograms of di-8 intensity (proportional to vesicle surface area) of EVs in rat plasma 1 hour and 24 hours after treatment with vehicle or LPS. Four replicate measurements are overlaid in each panel from a single representative sample. B. NTA diameter histograms of nanoparticles in rat plasma before and 24 hours after treatment with vehicle or LPS. Each panel shows the average histogram from a single representative sample. C. NTA population mean diameter (+/- SD) of nanoparticles in rat plasma as a function of time after treatment with vehicle or LPS.</p

Extracellular vesicle counts/concentrations in plasma over a 48 hour period.

<p>Total EV concentrations in control (●) and LPS (o) plasma as measured by VFC. (A) Total NP concentrations in control (●) and LPS (o) plasma as measured by NTA. (B) Marker positive EV concentrations in control and LPS plasma for annexin V (C), CD42d (D), CD54 (E), and CD106 (F). Presented are means and standard deviation for five animals at each time point. * indicate a significant (P<0.05) difference between the control and LPS-treated groups.</p

Complete blood count results from rats given saline vehicle or LPS.

<p>Complete blood count results from rats given saline vehicle or LPS.</p