6 research outputs found
Separation of Cells and Microparticles in Insulator-Based Electrokinetic Systems
Presented
here is the first continuous separation of microparticles
and cells of similar characteristics employing linear and nonlinear
electrokinetic phenomena in an insulator-based electrokinetic (iEK)
system. By utilizing devices with insulating features, which distort
the electric field distribution, it is possible to combine linear
and nonlinear EK phenomena, resulting in highly effective separation
schemes that leverage the new advancements in nonlinear electrophoresis.
This work combines mathematical modeling and experimentation to separate
four distinct binary mixtures of particles and cells. A computational
model with COMSOL Multiphysics was used to predict the retention times
(tR,p) of the particles and cells in iEK
devices. Then, the experimental separations were carried out using
the conditions identified with the model, where the experimental retention
time (tR,e) of the particles and cells
was measured. A total of four distinct separations of binary mixtures
were performed by increasing the level of difficulty. For the first
separation, two types of polystyrene microparticles, selected to mimic Escherichia coli and Saccharomyces
cerevisiae cells, were separated. By leveraging the
knowledge gathered from the first separation, a mixture of cells of
distinct domains and significant size differences, E. coli and S. cerevisiae, was successfully separated. The third separation also featured
cells of different domains but closer in size: Bacillus
cereus versus S. cerevisiae. The last separation included cells in the same domain and genus, B. cereus versus Bacillus subtilis. Separation results were evaluated in terms of number of plates
(N) and separation resolution (Rs), where Rs values for all
separations were above 1.5, illustrating complete separations. Experimental
results were in agreement with modeling results in terms of retention
times, with deviations in the 6–27% range, while the variation
between repetitions was between 2 and 18%, demonstrating good reproducibility.
This report is the first prediction of the retention time of cells
in iEK systems
Separation of Cells and Microparticles in Insulator-Based Electrokinetic Systems
Presented
here is the first continuous separation of microparticles
and cells of similar characteristics employing linear and nonlinear
electrokinetic phenomena in an insulator-based electrokinetic (iEK)
system. By utilizing devices with insulating features, which distort
the electric field distribution, it is possible to combine linear
and nonlinear EK phenomena, resulting in highly effective separation
schemes that leverage the new advancements in nonlinear electrophoresis.
This work combines mathematical modeling and experimentation to separate
four distinct binary mixtures of particles and cells. A computational
model with COMSOL Multiphysics was used to predict the retention times
(tR,p) of the particles and cells in iEK
devices. Then, the experimental separations were carried out using
the conditions identified with the model, where the experimental retention
time (tR,e) of the particles and cells
was measured. A total of four distinct separations of binary mixtures
were performed by increasing the level of difficulty. For the first
separation, two types of polystyrene microparticles, selected to mimic Escherichia coli and Saccharomyces
cerevisiae cells, were separated. By leveraging the
knowledge gathered from the first separation, a mixture of cells of
distinct domains and significant size differences, E. coli and S. cerevisiae, was successfully separated. The third separation also featured
cells of different domains but closer in size: Bacillus
cereus versus S. cerevisiae. The last separation included cells in the same domain and genus, B. cereus versus Bacillus subtilis. Separation results were evaluated in terms of number of plates
(N) and separation resolution (Rs), where Rs values for all
separations were above 1.5, illustrating complete separations. Experimental
results were in agreement with modeling results in terms of retention
times, with deviations in the 6–27% range, while the variation
between repetitions was between 2 and 18%, demonstrating good reproducibility.
This report is the first prediction of the retention time of cells
in iEK systems
Separation of Cells and Microparticles in Insulator-Based Electrokinetic Systems
Presented
here is the first continuous separation of microparticles
and cells of similar characteristics employing linear and nonlinear
electrokinetic phenomena in an insulator-based electrokinetic (iEK)
system. By utilizing devices with insulating features, which distort
the electric field distribution, it is possible to combine linear
and nonlinear EK phenomena, resulting in highly effective separation
schemes that leverage the new advancements in nonlinear electrophoresis.
This work combines mathematical modeling and experimentation to separate
four distinct binary mixtures of particles and cells. A computational
model with COMSOL Multiphysics was used to predict the retention times
(tR,p) of the particles and cells in iEK
devices. Then, the experimental separations were carried out using
the conditions identified with the model, where the experimental retention
time (tR,e) of the particles and cells
was measured. A total of four distinct separations of binary mixtures
were performed by increasing the level of difficulty. For the first
separation, two types of polystyrene microparticles, selected to mimic Escherichia coli and Saccharomyces
cerevisiae cells, were separated. By leveraging the
knowledge gathered from the first separation, a mixture of cells of
distinct domains and significant size differences, E. coli and S. cerevisiae, was successfully separated. The third separation also featured
cells of different domains but closer in size: Bacillus
cereus versus S. cerevisiae. The last separation included cells in the same domain and genus, B. cereus versus Bacillus subtilis. Separation results were evaluated in terms of number of plates
(N) and separation resolution (Rs), where Rs values for all
separations were above 1.5, illustrating complete separations. Experimental
results were in agreement with modeling results in terms of retention
times, with deviations in the 6–27% range, while the variation
between repetitions was between 2 and 18%, demonstrating good reproducibility.
This report is the first prediction of the retention time of cells
in iEK systems
Separation of Cells and Microparticles in Insulator-Based Electrokinetic Systems
Presented
here is the first continuous separation of microparticles
and cells of similar characteristics employing linear and nonlinear
electrokinetic phenomena in an insulator-based electrokinetic (iEK)
system. By utilizing devices with insulating features, which distort
the electric field distribution, it is possible to combine linear
and nonlinear EK phenomena, resulting in highly effective separation
schemes that leverage the new advancements in nonlinear electrophoresis.
This work combines mathematical modeling and experimentation to separate
four distinct binary mixtures of particles and cells. A computational
model with COMSOL Multiphysics was used to predict the retention times
(tR,p) of the particles and cells in iEK
devices. Then, the experimental separations were carried out using
the conditions identified with the model, where the experimental retention
time (tR,e) of the particles and cells
was measured. A total of four distinct separations of binary mixtures
were performed by increasing the level of difficulty. For the first
separation, two types of polystyrene microparticles, selected to mimic Escherichia coli and Saccharomyces
cerevisiae cells, were separated. By leveraging the
knowledge gathered from the first separation, a mixture of cells of
distinct domains and significant size differences, E. coli and S. cerevisiae, was successfully separated. The third separation also featured
cells of different domains but closer in size: Bacillus
cereus versus S. cerevisiae. The last separation included cells in the same domain and genus, B. cereus versus Bacillus subtilis. Separation results were evaluated in terms of number of plates
(N) and separation resolution (Rs), where Rs values for all
separations were above 1.5, illustrating complete separations. Experimental
results were in agreement with modeling results in terms of retention
times, with deviations in the 6–27% range, while the variation
between repetitions was between 2 and 18%, demonstrating good reproducibility.
This report is the first prediction of the retention time of cells
in iEK systems
Separation of Cells and Microparticles in Insulator-Based Electrokinetic Systems
Presented
here is the first continuous separation of microparticles
and cells of similar characteristics employing linear and nonlinear
electrokinetic phenomena in an insulator-based electrokinetic (iEK)
system. By utilizing devices with insulating features, which distort
the electric field distribution, it is possible to combine linear
and nonlinear EK phenomena, resulting in highly effective separation
schemes that leverage the new advancements in nonlinear electrophoresis.
This work combines mathematical modeling and experimentation to separate
four distinct binary mixtures of particles and cells. A computational
model with COMSOL Multiphysics was used to predict the retention times
(tR,p) of the particles and cells in iEK
devices. Then, the experimental separations were carried out using
the conditions identified with the model, where the experimental retention
time (tR,e) of the particles and cells
was measured. A total of four distinct separations of binary mixtures
were performed by increasing the level of difficulty. For the first
separation, two types of polystyrene microparticles, selected to mimic Escherichia coli and Saccharomyces
cerevisiae cells, were separated. By leveraging the
knowledge gathered from the first separation, a mixture of cells of
distinct domains and significant size differences, E. coli and S. cerevisiae, was successfully separated. The third separation also featured
cells of different domains but closer in size: Bacillus
cereus versus S. cerevisiae. The last separation included cells in the same domain and genus, B. cereus versus Bacillus subtilis. Separation results were evaluated in terms of number of plates
(N) and separation resolution (Rs), where Rs values for all
separations were above 1.5, illustrating complete separations. Experimental
results were in agreement with modeling results in terms of retention
times, with deviations in the 6–27% range, while the variation
between repetitions was between 2 and 18%, demonstrating good reproducibility.
This report is the first prediction of the retention time of cells
in iEK systems
Dependence of Nonlinear Electrophoresis on Particle Size and Electrical Charge
This study focuses on the dependence of nonlinear electrophoretic
migration of particles on the particle size and particle electrical
charge. This is the first report of the experimental assessment of
the mobilities of the nonlinear electrophoretic velocity of colloidal
polystyrene microparticles under two distinct electric field dependences.
A total of nine distinct types of polystyrene microparticles of varying
size and varying electrical charge were divided into two groups to
study separately the effects of particle size and the effects of particle
charge. The mobilities of the nonlinear electrophoretic velocity of
each particle type were determined in both the cubic and 3/2 regimes
(μEP,NL(3) and μEP,NL(3/2)). The results unveiled that both mobilities had
similar relationships with particle size and charge. The magnitude
of both μEP,NL(3) and μEP,NL(3/2) increased with increasing particle size and decreased
with increasing magnitude of particle charge. However, the observed
trends were not perfect as discussed in the Results and Discussion
section but still provide valuable information. These findings will
aid in the design of future size-based and charge-based separations
of particles and microorganisms