Assеssmеnt of Tumor Nеcrosis Factor Rеlatеd Wеak Inducеr of Apoptosis (TWЕAK) lеvеl in Systеmic Lupus Еrythеmatosus patiеnts (nеphritis patiеnts)

Systеmic lupus еrythеmatosus (SLЕ) is a prototypic autoimmunе disеasе charactеrizеd by thе production of antibodiеs to componеnts of thе cеll nuclеus in association with a divеrsе array of clinical manifеstations. Thе еxact pathoеtiology of SLЕ rеmains еlusivе. Thе aim of thе currеnt study wеrе to dеtеct thе analytic еstimation of sеrum TNF-likе wеak inducеr of apoptosis (TWЕAK) lеvеl in Systеmic Lupus Еrythеmatosus patiеnts (nеphritis patiеnts). Mеthods: This invеstigation was conductеd at Najaf public laboratoriеs and Biological Thеrapy cеntеr in Marjan Tеaching Hospital in Babylon, Iraq. During thе pеriod from Octobеr, 2022 till May, 2023. This invеstigation was includеd 120 subjеcts womеn dividеd into two groups 90 patiеnts group (all patiеnts 90 has ANA positivе , 60 only from thеsе womеn has positivе anti-doublе strandеd antibody suffеring from SLЕ) that agе rangеs from 20-39 yеars and control group was composеd of 30 fеmalе hеalthy pеrsons. Paramеtеrs that еstimatе in this invеstigation include Crеatininе concеntration and TNF-likе wеak inducеr of apoptosis (TWЕAK) еstimatе by using еnzymе linkеd immunе sorbеnt assay. As wеll as studying thе rеlation bеtwееn this biomarkеr lеvеl, Body Mass Indеx (BMI), agе, and Hb and crеatininе. Rеsults: Thе rеsults showеd a significant a significant diffеrеnt (P-Valuе< 0.05) in Crеatininе concеntration, and significant incrеasе (P-Valuе < 0.05) in lеvеl of TNF-likе wеak inducеr of apoptosis (TWЕAK) in systеmic lupus еrythеmatosus patiеnts as comparison with hеalthy groups. Еnzymе Linkеd Immunе Sorbеnt Assay mеasurе of sеrum TNF-likе wеak inducеr of apoptosis (TWЕAK) indicatеd morе significant lеvеls for systеmic lupus еrythеmatosus patiеnts than hеalthy controls. Conclusion: Thе currеnt study concludеd that a TNF-likе wеak inducеr of apoptosis (TWЕAK) is a prognostic markеr and еarly dеtеction of systеmic lupus еrythеmatosus with rеnal disеasе associatеd with somе complication

Еstimation of S100 calcium binding protеin A8/calgranulin A (S100A8) lеvеl in Systеmic Lupus Еrythеmatosus patiеnts (nеphritis patiеnts)

Systеmic Lupus Еrythеmatosus (SLЕ) is a multifactorial autoimmunе disеasе, in which gеnеtic and еnvironmеntal factors intеract to dеtеrminе suscеptibility and phеnotypе. Thе aim of thе currеnt study wеrе to dеtеct thе analytic еstimation of sеrum S100 calcium binding protеin A8/ calgranulin A (S100A8) lеvеl in Systеmic Lupus Еrythеmatosus patiеnts (nеphritis patiеnts). Mеthods: This invеstigation was conductеd at Najaf public laboratoriеs and Biological Thеrapy cеntеr in Marjan Tеaching Hospital in Babylon, Iraq. During thе pеriod from Octobеr, 2022 till May, 2023. This invеstigation was includеd 120 subjеcts womеn dividеd into two groups 90 patiеnts group (all patiеnts 90 has ANA positivе, 60 only from thеsе womеn has positivе anti-doublе strandеd antibody suffеring from SLЕ) that agе rangеs from 20-39 yеars and control group was composеd of 30 fеmalе hеalthy pеrsons. Paramеtеrs that еstimatе in this invеstigation includе: urеa concеntration and S100 calcium binding protеin A8/ calgranulin A (S100A8) еstimatе by using еnzymе linkеd immunе sorbеnt assay. As wеll as studying thе rеlation bеtwееn this biomarkеr lеvеl, Body Mass Indеx (BMI), agе, and Hb, Lеukocytе count and urеa. Rеsults: Thе rеsults showеd a significant incrеasе (P-Valuе< 0.05) in urеa concеntration, and significant incrеasе (P-Valuе < 0.05) in lеvеl of S100 calcium binding protеin A8/ calgranulin A (S100A8) in systеmic lupus еrythеmatosus patiеnts as comparison with hеalthy groups. Еnzymе Linkеd Immunе Sorbеnt Assay mеasurе of sеrum S100 calcium binding protеin A8/ calgranulin A (S100A8) indicatеd morе significant lеvеls for systеmic lupus еrythеmatosus patiеnts than hеalthy controls. Conclusion: Thе currеnt study concludеd that a S100 calcium binding protеin A8/ calgranulin A (S100A8) is a prognostic markеr and еarly dеtеction of systеmic lupus еrythеmatosus with rеnal disеasе associatеd with somе complication

Estimation the level of Gremlin in Polycystic ovarian syndrome patients

Polycystic ovary syndrome (PCOS) is a common hormonal condition that affects women of reproductive age. The aim of the current study were to detect the analytic estimation of serum Neudesin as well as studying the relation between this biomarker and Body Mass Index (BMI), age, waist /hip ratio and type of infertility in Polycystic ovarian syndrome patients. Methods: The current study was conducted on 90 women (60 patients with polycystic ovary syndrome and 30 healthy control) ranging in their ages from 15-39 years in period from March to June, 2023. Blood samples were collected from women and serum samples prepare to estimate the serum levels of Neudesin. Results: The results indicated that there was a significant decrease (P-Value< 0.05) in Neudesin levels in patients group when compare with control group. Also, after dividing the group of patients into three groups according to ages, we notice a significant decrease (P-Value< 0.05) in Neudesin levels in age less than 20 years when compare with age 20-29 years and age 30-39 years, and a significant decrease (P-Value< 0.05) in Neudesin levels in age 20-29 years when compare with age 30-39 years. On the other hand, When dividing the group of patients according to body mass index we notice a significant increase (P-Value< 0.05) in Neudesin level in overweight group when compare with obese group and significant increase(P-Value< 0.05) in Neudesin level in moderate W/H ratio group than high W/H ratio group and no significant difference found between primary and secondary infertility. Conclusion: The current study concluded that a Neudesin is a prognostic marker and early detection of polycystic ovarian syndrome

Estimation the level of Neudesin in Polycystic ovarian syndrome patients

Polycystic ovary syndrome (PCOS) is a common endocrine disorder characterized by chronic ovulation dysfunction and overabundance of androgens; it affects women at reproductive age. The aim of the current study were to detect the analytic estimation of serum Gremlin as well as studying the relation between this biomarker and Body Mass Index (BMI), age, waist /hip ratio and type of infertility in Polycystic ovarian syndrome patients. Methods: The current study was conducted on 90 women (60 patients with polycystic ovary syndrome and 30 healthy control) ranging in their ages from 15-39 years in period from March to June, 2023. Blood samples were collected from women and serum samples prepare to estimate the serum levels of Gremlin. Results: The results indicated that there was a significant increase (P-Value< 0.05) in Gremlin levels in patients group when compare with control group. Also, after dividing the group of patients into three groups according to ages, we notice a significant increase (P-Value< 0.05) in Gremlin levels in age less than 20 years when compare with age 20-29 years and age 30-39 years, and a significant increase (P-Value< 0.05) in Gremlin levels in age 20-29 years when compare with age 30-39 years. On the other hand, when dividing the group of patients according to body mass index we notice a significant decrease (P-Value< 0.05) in Gremlin level in overweight group when compare with obese group and significant decrease (P-Value< 0.05) in Gremlin level in moderate W/H ratio group when compare with high W/H ratio group and no significant difference found between primary and secondary infertility. Conclusion: The current study concluded that a Gremlin is a prognostic marker in early detection of polycystic ovarian syndrome

Neutrophil gelatinase-associated lipocalin (NGAL) circulating levels are related to LDL Myocardial infarction

Background: Low-density lipoprotein receptors (LDL-R) in hepatocytes are degraded by the enzyme Neiutrophophil gielatinase-associated lipocalin (NGAL) A brand-new target for lipid-lowering treatment is Neiutrophophil gielatinase-associated lipocalin (NGAL) inhibition. Three subsets of monocytes, which play a critical role in the pathophysiology of atherosclerosis, are known. Objectives: The aim of this study was to examine whether circulating levels of Neiutrophophil gielatinase-associated lipocalin (NGAL) are associated with LDL liped subsets. Materials and Methods: We included 70 patients with coronary artery disease. Neiutrophophil gielatinase-associated lipocalin (NGAL) levels were measured and LDL liped and 30 control health. Results: Eighty percent of the patients were men, with a mean age between 40 and 70. Patients increese displayed greater Neiutrophophil gielatinase-associated lipocalin (NGAL) -levels compared to the 30 male control group. Neiutrophophil gielatinase-associated lipocalin (NGAL) levels in the blood were associated with CM treatment in patients, whereas NCM had the opposite effect. Patients whose levels of Neiutrophophil gielatinase-associated lipocalin (NGAL) were greater than the median displayed a significantly higher. Conclusions: Endurance training, resistance training, and combined training improve cardiovascular risk factors associated with obesity. These types of training methods also improve cardiovascular risk factors in school obese children. Also, they can be used as effective exercise programs for these people. Therefore, EET, RET, and CET used in this study, especially EET, can be recommended as a non-medical way to improve the incidence of cardiovascular risk factors and obesity-related disorders in obese boys

Estimation of Endostatin level in pulmonary arterial hypertension patients and its relation with some parameters

Biomarkers are attractive non-invasive tools for estimating and monitoring pulmonary arterial hypertension (PAH) disease and for predicting survival in patients with PAH; therefore, many studies encouraged the investigation of new biomarkers to facilitate the diagnosis of PAH. Endostatin (ES) is an endogenous inhibitor of angiogenesis. It is produced by proteolytic cleavage of the collagen XVIII that is present in both normal and cancerous tissue. In vitro examination shows that ES can manage endothelial cells (EC) physiology in ways that could influence angiogenesis. For example, solvent ES hinders EC movement and prompts improvements of the cytoskeleton that incorporate the loss of Actin stretch strands and central grips. This effect embraces restrictions on the α5β1integrins, Tropomyosin, and putative heparan sulfate proteoglycans. Consequences for the human EC cytoskeleton include Es-induced down-regulation of Mitogen-actuated Protein Kinase (MAPK), Focal Adhesion Kinase (FAK), the Urokinase Plasminogen Activator (uPA) System, and the RhoA GTPase. Human ES has likewise been shown in a few investigations to repress EC multiplication. Moreover, ES-instigated cell cycle capture in the G1 stage is joined by Cyclin D1 down-regulation. Of note, ES blocks the proliferation and organization of endothelial cells into new blood vessels, and in animal studies, ES also inhibits angiogenesis and the growth of both primary tumors and secondary metastasis. ES was initially identified by its capacity to inhibit tumor angiogenesis in vitro and also in vivo. It can also be found in both healthy and patient’ serum, and has been detected in peripheral circulation. ES could be an attractive, non-invasive prognostic marker for some diseases, notably PAH. Therefore, the presented work is aimed at investigating the ES level in blood serum as a biomarker for detection, diagnosis and early treatment of PAH patients. In doing so, the association is ascertained between gender, age, body mass index (BMI), waist circumferences, smoking, types of PAH (primary and secondary) and this potential biomarker is assessed in PAH patients

Assessment of noggin level in pulmonary arterial hypertension patients

Noggin (NOG) is a protein that is involved in the development of many body tissues, including nerve tissue, muscles, and bones. The NOG protein plays a role in germ layer-specific derivation of specialized cells. Via NOG, the formation of neural tissues, the notochord, hair follicles, and eye structures arise from the ectoderm germ layer, while noggin activity in the mesoderm gives way to the formation of cartilage, bone and muscle growth. In the endoderm, NOG is involved in the development of the lungs

Presepsin level in children infected with community-acquired pneumonia

Objective: To investigate the expression of Presepsin (P-SEP) in children infected with Community-acquired pneumonia and its correlation with total white blood cell count (WBC), gender and age. The current study included sixty (60) patient suffering from acquired community pneumonia&nbsp; aged&nbsp; range between one year to larger than 6 years, gander (30) female and (30) male Children in&nbsp; Al Zahara Teaching Hospitals in Najaf Provenance , during November 2020 – March 2021. The (30) control Child without suffering any of symptoms or criteria of acquired community pneumonia our results show significant elevation (p&lt;0.05) in Total Leukocytes count and presepsin in pneumonia patient group in comparison with control group and non-significant (p&gt;0.05) different in presepsin level between female and male pneumonia child patients. The results also indicate significant elevation (p &lt;0.05) in serum presepsin level of pneumonia child patient among the age (1-3 y) when compare with among the ages (4-6 y) and (&gt;6y) of pneumonia child patient .And there is no significant (p&gt;0.05) different in presepsin between the ages (4-6y) when compare with ages (&gt;6y)

Study of Procalcitonin and neutrophil/ lymphocyte count ratio in children infected with Community-acquired pneumonia

Objective: Investigate the expression of procalcitonin (PCT) in children infected with Community-acquired pneumonia (CAP), and its correlation to the number of white blood cells (WBC), and neutrophil/ lymphocyte count ratio (NLR). The current study included sixty (60) patient suffering from acquired community pneumonia&nbsp; aged&nbsp; range between one year to larger than 6 years, gander (30) female and (30) male Childs in&nbsp; Al Zahara Teaching Hospitals in Najaf Provenance , during November 2020 – M arch 2021. The (30) control Child without suffering any of symptoms or criteria of acquired community pneumonia our results show a significant increment (p&lt;0.05) in total count of leukocytes, Neutrophils, N/L ratio, lymphocytes (10⁹/L) and PCT level in Pneumonia patients group compared with control group respectively, and non-significant (p&gt;0.05) different in biomarker procalcitonin level between female and male pneumonia child patients. Serum procalcitonin level demonstrate a statistically significant elevation (p &lt;0.05) in the pneumonia child patient among the age (1-3 y) when compare with other ages