Antimicrobial activity of the mint extract against campylobacter jejueni isolated from chicken sample

Foodborne infectious diseases are seen around the world. One of the most common contaminants found in chicken meat products is Campylobacter jejuni. This study tries to: To assess the utilization of Mint extract in various concentrations as natural antimicrobial against C. jejueni, 100 chicken samples were collected From the city center of AL- Diwaniyah, 50 samples of fresh poultry meat and 50 samples of frozen poultry meat, and the evaluation was done on them after preparation.The antimicrobial activity of methanolic, ethanolic, and aqueous extracts of mint was evaluated in multi-well micro-titer plates using the well diffusion method. Mint extract was evaluated for antimicrobial property against C. jejuni at different concentrations (80%, 40%, and 20%), and the highest inhibitory effect was observed in the ethanolic extract of mint, followed by the methanolic extract, while the aqueous extract was active only at a high concentration of 80% using the well diffusion method. Due to the biologically active properties of the compounds in mint, which are antimicrobial, antibacterial, anti-inflammatory, anticancer, antioxidant, and antifungal, mint extracts exhibit biological activity against C. jejueni

Effect the addition of Anis seed(Pimpinella anisum L.) powder on production performance and some blood and biochemical parameters in broiler chickens

This study was conducted at commercial farm for broiler which is located in Diwaniya city,from 1/4/2013 to 20/5/2013 to estimate the effect supplementation of two concentration of Anis seeds powder to feed  on production performance and some blood traits and blood biochemical parameters of broiler chickens by using (150) broiler chicken (Hubbard) at one day old which have been randomly distributed to three treatments by two replication per treatment(25 chicks per replication),this chicks divided into  the following:    T1:representing controle group eating the basal feed without addition of Anis seeds, While T2  representing the group which eating the basal feed plus 50 mg/kg of Anis seeds powder  to feed along the period of experiment and T3 representing the group which eating the basal feed plus 150 mg/kg of Anis seeds powder  to feed  along the period of experiment.The results  were revealed that Anis seeds powder supplementation to the feed in the (T2,T3) showed high significant increase( p<0.05) in body weight,gain weight,feed intake,dressing percentage compared with control treatment and significant decreasing( p<0.05) in mortality rate in T3 compared with control group.The addition of Anis seed powder lead to high significant increasing (p<0.05)in Hb concentration and packed cell volume %,total protein,albumin,globulin in treatment groups compared with control group

Molecular Characterization and Genotyping Study of Food Poisoning Staphylococcus aureus, Isolated from Raw Milk and Milk Products in Al-Diwaniyah, Iraq

Staphylococcus aureus still resembles one of the primary causes of food poisoning induced specifically by milk and its products. Recent studies from neighbor countries of Iraq showed the presence of this bacterium in the milk of cattle, sheep, and goat. The current study aimed to genetically identify S. aureus that was cultivated from 16 raw milk, 20 cheese, and 13 cream samples. The results show positive isolation of the bacterium in 6 (37.5%), 8 (40%), and 3(23%) samples respectively. Then, the isolates were subjected to multiplex polymerase chain reaction (mPCR) technique targeting enterotoxin genes, sea, seb, and seg. Interestingly, the milk samples showed amplification of these genes in 3 (33.3%), 1 (16.6%), and 4 (66.6%) isolates respectively. the cheese results revealed amplification of the genes in 4 (50%), 2 (25%), and 5 (62.5%) isolates respectively. Moreover, the cream indicated amplification of seg gene only in 1 (33.3%) isolate. Finally and to genotype the bacterium, the accessory gene regulator (agr) was employed to detect the bacterium nucleotide polymorphism. The results place the isolated S. aureus in different genotype groups but mostly in Group I and Group III. The current study results determine S. aureus as a pathogenic organism that thrives the milk and its products and might be responsible for many cases of food poisoning in the city