Mutation induced by gamma irradiation in coriander seeds and their identification by RAPD makers

This study was accomplished to detect variation generated through exposure of Coriander  ( Coriandrum sativum  L.) seeds to five  gamma irradiation doses (0,25,50,100 and150 ) GY by using eighteen  RAPDs primers as molecular marker ,results indicated that  primers OPC-05 ,OPC-19,  OPA-10,OPA-15 and OPB-06 success in giving three treatments a unique fingerprint  .Molecular size of amplified ranged between  1860 bp produced by primer OPA-04  and   115  bp    produced by primer OPW-04  . Highest degree for polymorphism was   91.66 % produced by primer OPA-04. Monomorphic bands appeared in their higher value in primer OPC-09 . Primer OPA-10 gave highest number   unique bands ( reached to seven bands )      Primer OPC-19 gave highest value for amplified bands  , primer OPA-10 gave highest value for main bands, Discrimination, in addition to  primer efficiency value  produced in their high value by primer OPA-04.This study revealed RAPD markers in detection mutation induced by gamma irradiation

Effect of gamma radiation on antioxidant enzymes and biochemicals of coriander (Coriandrum sativum L)

Coriander or Cilantro (Coriandrum sativum L.), is an annual herb that distinguishes with short life cycle , it displays broad adaptation as a crop around the world , growing well under many different types of soil and weather conditions. The analysis was carried out in 2022 in the laboratories of  Biology Department, College of Science under stable variables such as temperature, humidity, sunlight, grains of  Coriander   were irradiated with gamma rays at dose levels ( 25,50, 100 and 150 Gy) in addition to control treatment ( not irradiated )  using Cobalt 60 as irradiation source and planted in nursery of AL-Kufa university  to study the effects of gamma irradiation on the antioxidant enzymes (Antioxidant enzyme include Catalase (CAT), Superoxide dismutase (SOD) and Peroxidase (POD) in addition to several biochemicals (Chlorophyll a and b , α and β Carotenes , Proline and Malondialdehyde). In sum, the results here presented show that the effects of gamma radiation of   Coriander depend on the irradiation dose.&nbsp

Charecterization of mutation induced in Fenugreek by Gamma Ray using rapd marker

This study was accomplished to detect variation generated through eposure of fenugreek (trigonella foenum-graecum L.) seeds to seven gamma irradiation doses (0,25,50,100,150,300 and 400) GY by using twenty RAPDs primers as molecular marker ,results indicated that  primers OPA-14 ,OPC-19 and OPH-01success in giving five treatments a unique fingerprint. Molecular size of amplicon ranged between 2913 bp produced by primer OPB-06 and 118 bp produced by primer OPW-04.Highest degree for polymorphism was 90.92% produced by primer OPC-05. Monomorphic bands appeared in their higher value in primer OPC-08. Primer OPA-15 gave highest number unique bands Primer OPH-01 gave highest value for (amplified, main) bands, discrimination, in addition to primer efficiency value. Silght variation produce among primers OPE-02, OPC-05, OPH-01 and OPA-14. This study revealed RAPD markers in detection mutation induced by gamma irradiation