Evaluated the Up –regulation in Gene ‎Expression of Hepatic Insulin Gene and ‎Hepatic Insulin Receptor Gene in Type 1 ‎Diabetic Rats Treated with Cuscuta chinesis ‎Lam.‎

             انجزت هذه الدراسة للكشف عن فعالية مستخلص نبات الحامول الخافض للسكر  ودراسة ألية عمله على المستوى الجزيئي  والنسيجي  للاعضاء ذات العلاقة بمرض السكر( البنكرياس والكبد)، حيث تم قياس  العديد  من المؤشرات مثل مستوى سكر الدم الصيامى، مستوى الانسولين الصيامى في المصل، التعبير الجيني لكل من جين الانسولين في الكبد، جين الانسولين في البنكرياس و جين مستقبل الانسولين في الكبد بالاضافة الى اجراء فحوصات لمقاطع في انسجة البنكرياس والكبد. لاجراء هذه التجربة تم تقسيم اربعا وخمسون ذكراً من الجرذا ن نوع ٌRattus  rattus  والتي تراوحت معدلات  أوزنها من  200-180 غم  الى ثلاثة مجاميع رئيسية: مجموعة أ (مجموعة السيطرة للجرذان الطبيعي والتي تم تجريعها بالماء المقطر يومياً)، مجموعة ب(مجموعة السيطرة للجرذان المصابة  بداء السكري النوع الاول والتي تم تجريعها ايضاً بالماء المقطر يومياً) اما المجموعة الثالثة ج فكانت (مجموعة الجرذان المصابة  بداء السكري النوع الاول والتي تم تجريعها يومياً بالمستخلص الميثانولي بمقدار 400 ملغم/ كغم من وزن الجسم). تم تقسم كل مجموعة رئيسية والتي كانت تتالف من 18 جرذا الى ثلاث مجاميع ثانوية وفقا لمدة التجريع : مجوعة رقم (1) ، مجموعة رقم 2 ومجموعة رقم 3 لتشير الى عدد أيام التجريع 30 ، 60 و 90 يوما على التوالي ولقد تم قياس جميع المؤشرات السابقة وأوضحت النتائج بان التجريع اليومي بمستخلص نبات الحامول  400ملغم/كغم وزن جسم ادى الى الانخفاض المعنوي لمستوى سكر الدم الصيامي للمجموعة ج قياسا  بالمجموعة ب وكذلك رفع من مستوى كل من الانسولين الصيامى في المصل و، مستوى التعبير الجيني لكل من جين الانسولين في الكبد ، جين الانسولين في البنكرياس و جين مستقبل الانسولين في الكبد في المجموعة ج قياسا بالمجموعة ب ، كما ونلاحظ بانه اعاد جميع المؤشرات المدروسة في المجموعة ج الى الحالة الطبيعية بضمنها الطبيعة النسيجية لكل من النكرياس والكبد. استنتجت هذه الدراسة بان التجريع اليومي للجرذان المصابة بداء السكري من النوع الاول ب400ملغم ملغم/كغم من وزن الجسم من مستخلص نبات الحامول ولمدة 60 يوماً أعاد مستوى سكر الدم الصيامي الى مستواه الطبيعي من خلال رفع مستوى التعبير الجيني لكل من جين الانسولين في الكبد، جين الانسولين في البنكرياس  ،جين مستقبل الانسولين في الكبد ، اضافة الى ترميم خلايا بيتا في البنكرياس واعادة التركيب النسيجي لكل من البنكرياس والكبد الى الحالة الطبيعية        This research was conducted to study the hypoglycemic activity of C. chinesis Lam on type 1 diabetic disease and investigate the  molecular and histological mechanism of  its action .many parameters was investigated , Fasting blood glucose (FBG), Fasting serum insulin,Hepatic Insulin Gene Expression, pancreas Insulin Gene Expression ,Hepatic Insulin  Receptors Gene expression  and histological sections of pancrease and liver.54 Rattus rattus male rats weighting(180 -200g) were divided into 3 groups: A normal control daily administrated with Dw, B Diabetic control daily administrated with Dw  and C  diabetic group daily administrated with 400 mg/Kg body weight of C. chinesis  Lam. methanolic extract, each group consisted of  18 rats and further divided into (3) sub- groups 1 ,2  and 3. According to the period of administration  30, 60 and  90 days respectively. The results showing  the daily administration of 400 mg/Kg body weight of C. chinesis  Lam. methanolic extract for 60 day causing significance  decrease  in FBG and In the other hand each of fasting serum insulin, hepatic Insulin gene expression,pancreas Insulin gene expression and hepatic Insulin receptor gene expression was increased in group C in compare to B group and return all studied parameters involving pancrease and liver texture to the normal state ,which were statically morphologically  not appeared any significant difference from A group .this study concluded that the daily administration type 1 diabetic rats with 400 mg/Kg body weight of C. chinesis  Lam. extract for 60 day was return  fasting serum insulin and FBG to normal value by  upregulated  the gene expression of hepatic INS Gene ,INSR gene , pancreas INS Gene ,regenerate pancreatic beta- cell and returnthe texture of both liver and pancrease to the normal stat

Effect of Uric Acid and Lipid Profile on Myocardial Infarction in Iraqi Population

Myocardial infarction is a common presentation of coronary artery disease. This study was aimed to evaluate the effect of uric acid and lipid profile on myocardial infarction in Iraqi population. The study was conducted on (30) patients acute myocardial infarction, and (30) apparently healthy subjects were taken as control group. The level of uric acid and lipid profile was determined using colorimetric method. Patient group had significantly higher level of uric acid (P < 0.01), total cholesterol (P < 0.01), triglyceride (P < 0.01), low density lipoprotein (P < 0.01), very low density lipoprotein (P < 0.01) and significantly lower level of high density lipoprotein (P < 0.01) than control group. The results of this study also showed a significant difference (P < 0.01) between males and females regarding uric acid, total cholesterol, triglyceride, low density lipoprotein, very low density lipoprotein and high density lipoprotein in both study groups. The body weight of the patients demonstrated as body mass index showed a significant association (P < 0.01) with the level of uric acid, total cholesterol, triglyceride, low density lipoprotein and high density lipoprotein in patient group. In conclusion, uric acid may have an effect on precipitating some risk factors of myocardial infarction. Key words: Uric acid, lipid profile, myocardial infarction

Inhibition of cyclophosphomide mutagenicity using Glycyrrihza glabra root extract

The aims of the studying is using methanolic - water extract of the Glycyrrihza glabra root for inhibition the mutagenicity of anticancer drug by cytogenetic assays in vivo in three interaction between the drug and extract. The result shows that the extract is bioantimutagenesis. The treatment by extract after drug causes increase mitotic index and decrease chromosome aberration and sperm head abnormalities. The thin layer chromatography of the extract show that is contains different polar compounds

Evaluation of Some Biomarker and Genetic Marker in Myocardial Infraction Patients

Acute coronary syndrome (ACS) consists of unstable angina pectoris, non-ST segment elevation myocardial infarct ion and ST segment elevate ion myocardial infarction.  The aim of the study was to compare the vibration  some hormones levels in the Acute coronary syndrome patients and control groups and This study aimed to investigate the association between two single nucleotide polymorphisms (?1562C>T, of the MMP-9 gene in patients with ACS in the Iraq population. Subjects and methods: This study was conducted between / mrjan teaching hospital in Babylon Province between November 2012- Aprial 2013and it was carried out at the coronary care unit  / in Babylon province/Iraq .  This patient-control study was composed of 60 ACS patients with age 53.38 ± 9.51 and 30control withe age  51.43 ± 7.81subjects, The present study is divided into two main parts: physio-biochemical and molecular parts. The physiological part involved hormonal assay( troponin and endothelin-1) while molecular part included The genotypes of the  selected SNPs were determined by the method of polymerase chain reaction and restriction fragment length polymorphism (RFLP-PCR). The relationship between the polymorphism of the MMP-9 gene and the severity of Acute coronary syndrome with negative medical history. The result of this study showed that patients with Acute coronary syndrome both males and females had significantly troponin and elevated Endothelin -1 in patient than control<0.001**(**p value ? 0.01 was significant).Analysis of the SNPs showed that the frequency of CT and TT genotypes in patients with ACS was significantly higher than that in the control group (ACS vs. controls; CT+TT: (85%Vs 36%). Keywords:single nucleotide polymorphisms, troponin, endotheline, MMP-

Cytotoxic and Genotoxic Effect of Aflatoxin B1

The aims of study is to determine cytogenetic and genotoxic effect of aflatoxin B1 in vivo, cytogenetic tests are used in studying include chromosome malformation, cell proliferation and plast index test in bone marrow cell in Wight albino rats, the results show that toxin causes Varity in chromosome malformation and increased the rate of it, so it increased in cell proliferation and plast index value compare with negative control

Molecular Characterization of Restriction Endonuclease Extracted from Local E.coli Isolate

An endonucleases enzyme named EcoRHI has been purified partially from one of pathogenic E.coli isolate about 87-fold with endonuclease recovery about 12.6% . The purification steps included ammonium sulfate precipitation with 50% saturation ratio, and the enzymatic solution reclaimed it through Sephadex G-100 column .The SDS-PAGE analysis that showed molecular weight of EcoRHI enzyme is 45 KDa. The purified endonuclease was able to digest λ-DNA into two fragments

The Prevalence of Microalbuminuria in Type 2 Diabetes Mellitus Patients in Al-Husain Hospital in Karbala Province- Iraq

This study is performed to assessment of the long term glycaemic hemoglobin (HbA1c) to determine the prevalence of microalbuminuria and to find the risk factors for developing microalbuminuria and consequence nephropathy in patients with type 2 diabetes. The prevalence of microalbuminuria in our study is high (59%) and the percent of and the risk factors that accompanied microalbuminuria are high blood pressure, elevated fasting blood glucose and poor glycemic Diabetes mellitus (D.M.) is a group of metabolic diseases characterized by hyperglycemia poor glycemic control also high  (92.3%) with control

Total and differential leukocytes count in type 2 diabetes mellitus patients in Iraq

To find the correlation between total and differential leukocytes count and the presence of microalbuminuria in diabetic patients. The results show significant positive correlation between total leukocytes count and microalbuminuria in males and females also there is significant positive correlation between neutrophils count and microalbuminuria in males and females but there is no correlation with other types of leukocytes

Protective Activity of Glycyrrihzaglabra against Histopathological Changes in White Albino Rats

The study was aimsto evaluation the protective activity of methanol-water extract of Glycyrrihzaglabraroot (GL) against histopathological changes induced by cyclophosphamide in white albino rats. Multiple doses for drug and extract were used to investigate the accumulation effect of drug and extract onliver and kidney. Results show that drug causes different harmful changes in rat organs in all doses concentrations used in study and extract have protective activity to prevent changes in tissue in 1000, 750 and 250 mg \kg , but dose 500 was failure to protect liver and lowest effect on kidney. Conclusion of present study is Glycyrrihzaglabrauseful for protective body organs against side effect of drugs and harmful effect of oxidative stress. Keywords: Glycyrrihzaglabra  ,cyclophosphamide, liver, kidney