Prevalence of sleep-disordered breathing and associations with orofacial symptoms among Saudi primary school children

Abstract Background This study aimed to determine the prevalence of sleep-disordered breathing among primary school children in Riyadh, Saudi Arabia, and to evaluate associations between sleep-disordered breathing and respiratory conditions/orofacial symptoms. Methods In this cross-sectional study, 1600 questionnaires were distributed to Saudi boys and girls aged 6–12 years from 16 primary schools in Riyadh. The questionnaire covered relevant demographic and personal characteristics, presence of respiratory conditions and orofacial symptoms, and the Pediatric Sleep Questionnaire. The latter was used to assess the prevalence of symptoms of sleep-disordered breathing and was completed by the participating children’s parents. Results In total, 1350 completed questionnaires were returned (85% response rate). The children’ mean age was 9.2 ± 1.8 years; 733 (54.3%) were boys and 617 (45.7%) girls. Overall, 21% of children were at high risk of sleep-disordered breathing. The prevalence of snoring was 14.4% and that of sleep apnea 3.4%. Boys were at higher risk of sleep-disordered breathing than girls (P = 0.040). Children with respiratory conditions or orofacial symptoms were at higher risk of sleep-disordered breathing (P < 0.0001) than children without these conditions/symptoms. Conclusions Around 21% of Saudi children are at risk of sleep-disordered breathing. There is a strong association between sleep-disordered breathing symptoms and the presence of respiratory conditions or orofacial symptoms

Correlation between skeletal maturation and developmental stages of canines and third molars among Saudi subjects

Aims: The present study was designed to evaluate the efficacy of using the developmental stages of the canines and third molars to predict the timing of skeletal maturity in the Saudi population. Material and methods: The lateral cephalometric radiographs and orthopantograms of 239 Saudi patients, 106 males and 133 females, aged 9 to 21 years, were collected from several dental centers. Orthopantograms were used to assess the developmental stages of the upper and lower canine teeth and third molars using two popular methods: that of Nolla and that of Demirjian. Cervical vertebral maturation (CVM) stage was assessed on the lateral cephalometric images according to the method of Baccetti et al. Trained observers with no knowledge of patient age or gender performed assessments. Data were analyzed with Spearman’s rank correlation coefficient at a significance level of P ≤ .05. Result: Skeletal CVM stages III and IV had a stronger correlation with mandibular left canine developmental stage than with maxillary canine developmental stage in the two methods used (correlation with Nolla stage 10 and Demirjian stage H: root completely formed with apex closed), especially for male patients (r = 0.700, P < .001). In contrast, the maxillary third molars at Nolla stages 5 and 7 (crown completed to 1/3 of the root formed) showed an association with CVM stages III and IV (r = 0.540 for females and r = 0.639 for males, P ≤ .001 for both) and with Demirjian stages D, E, and F. Males had slightly higher correlation values than females (r = 0.578 and 0.5010, respectively; P ≤ .001) at CVM stages III and IV. Interestingly, canine teeth showed a stronger correlation than third molars with skeletal maturation in Saudi children. Conclusion: Dental developmental stages were highly correlated with CVM stages III and IV among Saudi subjects. Keywords: Cervical vertebral maturation, Nolla, Demirjian, Developmental stages, Cephalometric, Canines, Third mola

Association between polymorphisms in PRNCR1 and risk of colorectal cancer in the Saudi population.

LncRNA Prostate cancer non-coding RNA (PRNCR1) is downregulated in many types of cancer. The current case-control study was performed on 144 patients with colorectal cancer and 130 matching controls. Genotyping was performed using TaqMan assays for four Single Nucleotide Polymorphisms (SNPs) in PRNCR1. RNAsnp Web Server was used to detect variations in the secondary structure for each SNP. The genotyping analysis for SNP rs1456315 showed increased association with colorectal cancer with the homozygous CC variant allele (OR: 2.09; χ2 = 4.95; CI: 1.08-4.02; p = 0.02), the minor allele frequency, and additive genotype, respectively (OR: 1.55; χ2 = 6.24; CI: 1.09-2.19; p = 0.01) & (OR: 1.64; χ2 = 4.04; CI: 1.01-2.67; p = 0.04). A risk association was also observed among younger age patients (≤57) and in female patients as well as in patients with tumors of the colon. For the other SNPs tested (rs16901946, rs13252298, rs1016343), no significant association was observed. The secondary structure of the rs1456315 mutant is different from that of the wild-type. Our findings suggest that the upregulation of PRNCR1 and its variants is associated with increased risk of colorectal cancer in Saudi patients, indicating that PRNCR1 might be a unique and valuable signature for predicting the risk of colorectal cancer in a Saudi population