The hemolytic propertiesof clinical isolates of Morganella morganii

Morganella morganii is a gram-negative bacterium from the Enterobacteriaceae family which causes a wide range of clinical infections sometimes with fatal consequences. It is known that more than 50% of isolates of M. morganii from clinical specimens have hemolytic activity that increase their virulence. Pore-forming toxins (PFT) represent the most common group of cytotoxic proteins which contribute the delivering of the bacterial proteins into host cells, loss of nutrients and ions by eukaryotic cells, as well as the exit of bacteria from phagosome into cytosol. In this study we investigated the hemolytic activity of two M. morganii strains. It has been shown that hemolytic activity for strain of M. morganii 4 is 3 times higher than for strain of M. morganii 1. The maximum hemolytic activity is observed in LB medium but synthesis of hemolysins is higher in synthetic urine. Finally, the PCR-analysis of 5 hypothetical hemolysin genes has shown that strain M. morganii 1 does not contain homologous of α-hemolysin from E. coli that may explain the observed differences in hemolytic activity of the investigated strains

The hemolytic propertiesof clinical isolates of Morganella morganii

Morganella morganii is a gram-negative bacterium from the Enterobacteriaceae family which causes a wide range of clinical infections sometimes with fatal consequences. It is known that more than 50% of isolates of M. morganii from clinical specimens have hemolytic activity that increase their virulence. Pore-forming toxins (PFT) represent the most common group of cytotoxic proteins which contribute the delivering of the bacterial proteins into host cells, loss of nutrients and ions by eukaryotic cells, as well as the exit of bacteria from phagosome into cytosol. In this study we investigated the hemolytic activity of two M. morganii strains. It has been shown that hemolytic activity for strain of M. morganii 4 is 3 times higher than for strain of M. morganii 1. The maximum hemolytic activity is observed in LB medium but synthesis of hemolysins is higher in synthetic urine. Finally, the PCR-analysis of 5 hypothetical hemolysin genes has shown that strain M. morganii 1 does not contain homologous of α-hemolysin from E. coli that may explain the observed differences in hemolytic activity of the investigated strains

The hemolytic propertiesof clinical isolates of Morganella morganii

Morganella morganii is a gram-negative bacterium from the Enterobacteriaceae family which causes a wide range of clinical infections sometimes with fatal consequences. It is known that more than 50% of isolates of M. morganii from clinical specimens have hemolytic activity that increase their virulence. Pore-forming toxins (PFT) represent the most common group of cytotoxic proteins which contribute the delivering of the bacterial proteins into host cells, loss of nutrients and ions by eukaryotic cells, as well as the exit of bacteria from phagosome into cytosol. In this study we investigated the hemolytic activity of two M. morganii strains. It has been shown that hemolytic activity for strain of M. morganii 4 is 3 times higher than for strain of M. morganii 1. The maximum hemolytic activity is observed in LB medium but synthesis of hemolysins is higher in synthetic urine. Finally, the PCR-analysis of 5 hypothetical hemolysin genes has shown that strain M. morganii 1 does not contain homologous of α-hemolysin from E. coli that may explain the observed differences in hemolytic activity of the investigated strains

The hemolytic propertiesof clinical isolates of Morganella morganii

Morganella morganii is a gram-negative bacterium from the Enterobacteriaceae family which causes a wide range of clinical infections sometimes with fatal consequences. It is known that more than 50% of isolates of M. morganii from clinical specimens have hemolytic activity that increase their virulence. Pore-forming toxins (PFT) represent the most common group of cytotoxic proteins which contribute the delivering of the bacterial proteins into host cells, loss of nutrients and ions by eukaryotic cells, as well as the exit of bacteria from phagosome into cytosol. In this study we investigated the hemolytic activity of two M. morganii strains. It has been shown that hemolytic activity for strain of M. morganii 4 is 3 times higher than for strain of M. morganii 1. The maximum hemolytic activity is observed in LB medium but synthesis of hemolysins is higher in synthetic urine. Finally, the PCR-analysis of 5 hypothetical hemolysin genes has shown that strain M. morganii 1 does not contain homologous of α-hemolysin from E. coli that may explain the observed differences in hemolytic activity of the investigated strains

Phenotypic Investigation of Vancomycin, Teicoplanin and Linezolid Resistance Among Enterococcus spp. Isolated from Children Diarrhea

Vancomycin-resistant Enterococci(VRE) were common among Enterococcus. faecalis and Enterococcus faecium. Teicoplanin resistance or sensitivity can determine the VRE phenotypes whether VanA (VanR/TecR) or VanB (VanR/TecS). Linezolid resistance among VRE regards an newly emerged health problem. Infection with LRVRE or TRLRVRE pushan hazardous alert for hard to heal illness. Twenty eight Enterococcus spp. isolates were recovered from children diarrhea after their inoculation on m-EI chromogenic agar. Antibiotics susceptibility and phenotypic detection of antibiotics resistance were performed according to CLSI 2016. The results revealed 92.86% resistance to rifampin, 85.71% to erythromycin. VRE were 46.42%, TRE were 25% and LRE were 35.71% while co-existed resistance for Vancomycin/Teicoplanin/Linezolid(TRLRVRE) were detected 25% in. concern antibiotics resistant patterns, the MDR compile (85.7%) while XDR compile (10.7%) and there is no PDR among Enterococcus. spp. isolates were PDR. The presentstudy conclude that VanA and VanB phenotypes were common among MDR and XDR and although there is no using of linezolid but the emergence of TRLRVRE isolates were stated