Z-adjusted values of dynamic mechanic allodynia measured in quantitative sensory testing in FMS patients relative to healthy controls.

**p<0.001; we used ANOVA for statistical testing. DMA = dynamic mechanical allodynia.</p

Alpha-diversity statistics for the microbiome in FMS patients vs. healthy controls.

Alpha-diversity statistics for the microbiome in FMS patients vs. healthy controls.</p

Demographic data (age, sex, BMI, smoking status) and dietary, medication and psychometric profiles of patients with FMS vs healthy controls.

Demographic data (age, sex, BMI, smoking status) and dietary, medication and psychometric profiles of patients with FMS vs healthy controls.</p

Statistical analysis of microbial beta-diversity in FMS patients vs healthy controls.

Statistical analysis of microbial beta-diversity in FMS patients vs healthy controls.</p

Flow chart of how many study participants were included/excluded in the study.

* 8 were excluded in the FMS-group: 1 Ehlers-Danlos syndrome, 1 unclear autoimmune disease, 1 stroke in anamnesis, 1 acute severe pneumonia, 2 non-compliant, 2 lost contact. ** 1 was excluded in the healthy control (HC) group: autoimmune disease.</p

Taxonomic composition of bacterial phyla in healthy controls.

For healthy controls, the predominant phyla were Bacteroidetes (44%), Firmicutes (41%) and Proteobacteria (8%). All phyla were tested for differences with DeSeq2 (GMPR normalization) which did not reveal any significant results. Abbreviations: gmpr–geometric mean of pairwise ratios.</p

Z-adjusted values of paradoxical heat sensations measured in quantitative sensory testing in FMS patients relative to healthy controls.

We used ANOVA for statistical testing. PHS = paradoxical heat sensations.</p

Canonical correspondence analysis (CCA) of the microbiota.

CCA analysis of microbiome in FMS patients and healthy controls (HC) failed to reveal a distinct “cluster” to each group.</p

S1 File -

(XLSX)</p

Results of quantitative sensory testing of FMS patients vs healthy controls (HC, raw data).

Results of quantitative sensory testing of FMS patients vs healthy controls (HC, raw data).</p