Genetic diversity and relationship analysis among accessions of Aegilops ssp in Turkey using amplified fragment length polymorphism (AFLP) markers

Amplified fragment length polymorphism (AFLP) DNA markers were used to assess the genetic diversity and relationships between 55 accessions of genus Aegilops, including the species Aegilops triuncialis L. (UUCC), Aegilops geniculata Roth (MMUU), Aegilops cylindrica Host (CCDD) and Aegilops umbellulata Zhuk (UU). The samples were collected from Aegean region and East Anatolia, Turkey. 16 AFLP selective primer combinations generated a total of 3200 polymorphic amplification products. 50 Aegilops accessions were analyzed using the data analysis software, unweighted pair-group method arithmetic average (UPGMA) method and numerical taxonomy and multivariate analysis system (NTSYSpc-2.02k). The similarity index coefficients were calculated according to simple matching coefficient. Using 16 AFLP primer combinations, species from Aegean region and east Anatolia were clustered as four major groups. Aegilops species having U genome clustered together and A. cylindrica host was out grouped

Buğdayda Sorun Olan Septorya Yaprak Lekesi (Zymoseptoria tritici) Patojeni'nde Fungal Efektör Adayı Bazı SSP (Small Secreted Protein) Genlerinin Fonksiyonel Analizi

TÜBİTAK TOVAG Proje15.12.2017Septorya yaprak leke hastalığı etmeni Zymoseptoria tritici ülkemizde buğdayın en önemliyaprak hastalıklarından birisidir. Askomiset, hemibiotrof bir fungus olan fungusun tam genom sekans analizleri tamamlanmış ve erişime açılmıştır. Fungusun genomundaki genlerin fonksiyonlarının ve bitkiyi hastalandırma sürecinde görev alan efektör genlerinin belirlenmesi fungusa karşı mücadele stratejilerinin geliştirilmesinde büyük önem arz etmektedir.Bu proje buğday yaprak lekesi etmeni Z. tritici genomunda yer alan bazı küçük, hücre arasına salınan proteinleri kodlayan, fungal efektör adayı genlerin (Small Secreted Protein- SSP) gen silme tekniği ile fungusun genomundan silinip, fungusun biyolojik ve patolojik özelliklerine etkilerinin belirlenmesi amacıyla gerçekleştirilmiştir. Hedef SSP genlerine ait nükleotid dizileri kullanılarak oluşturulan gen silme kasetleri ve Agrobacterium temelli transformasyon tekniği kullanılarak patojenin genomundan hedeflenen genlerin tamamı silinebilmiştir.Silinen SSP genlerinden biri (Zt-3) fungusun temel biyolojik özelliklerinden biri olan mayaformunda gelişim özelliğini önemli oranda etkilemiştir. Zt-3 geni silinmiş izolat sadece makro piknidiospor üretebilmiştir. Çalışılan genlerin fungusun hastalandırma yeteneği üzerinde etkili olup olmadığı, geni silinmiş izolatlar ile yapılan patojenisite testleri ile araştırılmıştır. Herhangi bir etki bulunamamıştır. Çalışma sonunda Z. tritici gen fonksiyonu çalışmalarında gen silme tekniği ülkemizde ilk defa kullanılmış ve optimize edilmiştir. Yapılan çalışma bu tekniğin fungal genlerin fonksiyonlarının belirlenmesinde başarılı ve etkin bir şekilde kullanılabileceğini ortaya koymuştur.Septoria leaf blotch is one of the most important leaf diseases of wheat in Turkey.Ascomycet and hemibiotrophic fungus was sequenced as whole genome andrelease in public. It is very important to find out functions of the fungal genes andeffector proteins having roles in pathogenicity to develop new managementstrategies against fungus.This project was carried out to knock out of some Small Secreted Proteins (SSP)genes and examine the effects of gene deletion process on biological andpathogenic characteristics of it. Target SSP genes were successfully deletedusing gene deletion cassettes produced using nucleotide sequences of SSPgenes and Agrobacterium mediated transformation.One of deleted SSP genes (Zt-3) effected to development of yeast form of thefungus in important scale. Mutant isolate could only produce macropicniospores.It was investigated for the selected SSP genes whether gene deletion causedany difference on fungal pathogenicity using plant tests. Results showed therewas no effects of deletion of these genes.At the end of the study, gene deletion technique was firstly optimized and used ingene function studies on Z. tritici. This study showed that this technology can beused properly and effectively on gene function studies of the fungi

Dexamethasone and Basic‐Fibroblast Growth Factor Regulate Markers of Mineralization in Cementoblasts In Vitro

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/142252/1/jper1550.pd

Remarkable phosphodiester hydrolysis activity of a novel Ce-IV complex in neutral aqueous solutions

A novel Ce-IV-complex of 1,4,7-tris(carbamoylmethyl)-1,4,7-triazacyclononane has been synthesized. The complex is both stable in aqueous solutions at neutral pH and very efficient in promoting the hydrolysis of a phosphodiester model compound and yeast tRNA(phe). The RNA model compound, 2-hydroxypropyl-p-nitrophenylphosphate hydrolysis is accelerated 7400-fold at pH 7.5, as a result of an unprecedented hydrolytic activity

Remarkable cooperative action of two zinc centers in the hydrolysis of plasmid DNA

A novel binuclear zinc complex has been synthesized. The complex is highly efficient in the hydrolysis of plasmid DNA at pH 7.5. Furthermore, a comparison to a mononuclear complex reveals a high level of cooperativity between the two metal ion centers

RT-PCR amplification of a Rhizopus oryzae lactate dehydrogenase gene fragment

No amino acid or DNA sequence information in sequence databases was found for a fungal lactate dehydrogenase (LDH) isozyme. Highly conserved regions in the lactate dehydrogenase enzymes of all taxonomies are found to be beta alpha beta nucleotide binding and substrate binding sites, also catalysis/active site. The conserved regions were selected as PCR primer target regions. The degenerate primers were designed according to the codon usage, determined by analyzing a number of different genes of Rhizopus species. A fragment of the gene (ldh), coding for similar to 72% of the lactate dehydrogenase enzyme from Rhizopus oryzae, was amplified using degenerate primers by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). The size of the amplified fragment containing beta alpha beta nucleotide binding site, substrate binding site and catalysis/active site is found to be about 700 bp. The reported degenerate PCR primers and the amplification conditions may lead to the cloning of the lactate dehydrogenase gene of R. oryzae, which is an important organism due to its utilization in lactic acid and enzyme productions in industrial scales. (C) 2001 Elsevier Science Inc. All rights reserved

FEN BİLİMLERİ ENSTİTÜSÜ/LİSANSÜSTÜ TEZ PROJESİ

SARIPAS PATOJENİ EFEKTÖRÜ İLE İLİŞKİDE OLAN KONUKÇU BİTKİ FAKTÖRLERİNİN SAPTANMAS

FEN BİLİMLERİ ENSTİTÜSÜ/LİSANSÜSTÜ TEZ PROJESİ

BUĞDAY DİRENÇ GENİ YR10 İLE ETKİLEŞEN PATOJEN PROTEİNLERİNİN SAPTANMAS

FEN BİLİMLERİ ENSTİTÜSÜ/LİSANSÜSTÜ TEZ PROJESİ

CLONİNG,SEQUENCİNG AND VERİFİCATİON OF İDENTİTİES OF THE EFFECTOR GENES İN PUCCİNİA STRİİFORMİS F.SP.TRİTİC