OXIDATIVE ENZYMES IN PARAFFIN SECTIONS

この論文は国立情報学研究所の学術雑誌公開支援事業により電子化されました。The usefulness of paraffin sections was shown for the histochemical detection of some oxidative enzymes. The acetone fixed paraffin sections were floated on the incubating mixture containing Nitro-BT, substrates and others without sticking on the slide or cover glass, or stored in a refrigerator until use. DPN-and TPN-diaphorase (DPND, TPND), DPN-and TPN-linked dehydrogenase systems and succinic dehydrogenase system were examined. The diaphorases and succinic dehydrogenase have shown their marked activities and other dehydrogenases in various grades but not so prominent. The present procedures will give the sufficient results for the detection of oxidative enzymes in the lipid-rich tissues such as the adrenal cortex. It seems most likely that the success of the present studies might be due to the use of Nitro-BT for the hydrogen acceptor and to floating the paraffin sections directly on the incubating mixtures. Thus, the present procedures made it possible to stock the tissues maintaining the oxidative enzyme activities and to avoid or minimize the diffusion of the enzymes and produced formazan deposits

ホウブツガタ ホウテイシキ ニタイスル TROTTER KATOガタキンジ

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BALL DISTRIBUTION USING THE INSTEP KICK IN COLLEGIATE SOCCER PLAYERS

The present study investigates the characteristics of the distribution of the soccer ball during the instep kick to clarify the reproducibility of the ball distribution. Six male collegiate soccer players kicked a soccer ball towards a goal 11 m away (5 sets of 10 trials). Ball distribution was then analyzed using a high-speed camera. In relation to ball velocity, no differences were seen between sets in the distribution distance from the center of the goal (in either the x-axis or the y-axis direction) among all subjects. High reproducibility was observed for all items. Furthermore, no differences were observed between sets in the slopes and intercepts of the regression equations for the ball distribution. These values also showed high reproducibility. An elliptical shape from the lower left to the upper right was revealed after analysis of ball distribution for the instep kick

タイカ ホウブツガタ ホウテイシキ ノ ユウゲン ヨウソ キンジ

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