Strongly invertible knots, rational-fold branched coverings and hyperbolic spatial graphs

A construction of a spatial graph from a strongly invertible knot was developed by the second author, and a necessary and sufficient condition for the given spatial graph to be hyperbolic was provided as well. The condition is improved in this paper. This enable us to show that certain classes of knots can yield hyperbolic spatial graphs via the construction.Comment: 18 pages, 3 figures; proofs of several results, including Proposition 2 and Lemma 6 has been improved. Mail theorem is also improve

Net baryon number fluctuations across the chiral phase transition at finite density in the strong coupling lattice QCD

We investigate the net-baryon number fluctuations across the chiral phase transition at finite density in the strong coupling and chiral limit. Mesonic field fluctuations are taken into account by using the auxiliary field Monte-Carlo method. We find that the higher-order cumulant ratios, $S\sigma$ and $\kappa\sigma^2$, show oscillatory behavior around the phase boundary at $\mu/T\gtrsim 0.2$, and there exists the region where the higher-order cumulant ratios are negative. The negative region of $\kappa\sigma^2$ is found to shrink with increasing lattice size. This behavior agrees with the expectations from the scaling analysis.Comment: 16 pages, 5 figure

Electron microscopy of 26 S complex containing 20 S proteasome

AbstractA high molecular weight protease complex (26 S complex) involved in the intracellular protein degradation of ubiquitinated proteins was purified from rat liver and studied by electron microscopy. The most prevalent molecular species with best preserved symmetrical morphology had two large rectangular terminal structures attached to a thinner central one having four protein layers. We concluded that they were the closest representation of the 26 S complex so far reported. The central structure was identified as 20 S proteasome and the terminal one as recognition units for ubiquitinated proteins

ATP-dependent reversible association of proteasomes with multiple protein components to form 26S complexes that degrade ubiquitinated proteins in human HL-60 cells

AbstractThe role of proteasomes in ubiquitin (Ub)-dependent protein degradation was studied by analyzing lysates of human promyelocytic leukemia HL-60 cells by glycerol density gradient centrifugation. High succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide hydrolyzing activity was found in the 26S fraction, whereas the 20S fraction containing proteaomes had no activity. Addition of 0.05% sodium dodecylsulfate to the latter fraction, however, induced marked activity. The 26S, but not the 20S fraction catalyzed ATP-dependent degradation of [125I]lysozyme-Ub conjugate. Depletion from the lysate of ATP caused complete shift of the active 26S complex to the latent 20S form, whereas in the lysate prepared from ATP-depleted cells, ATP converted 20S proteasomes to 26S complexes. The immunoprecipitated 26S complexes were found to consist of proteasomes and 13–15 other proteins ranging in size from 35 to 110 kDa. We conclude that in the lysate, latent proteasomes undergo reversible, ATP-dependent association with multiple protein components to form 26S complexes that catalyze ATP-dependent degradation of Ub-protein conjugates

Can CT colonography be useful for colon cancer screening?

For early detection and treatment of colon cancer, increasing of cancer screening rates is an urgent task. We started CT colonography（CTC）in September ２０１５ and have examined ３０９ cases for１６months. CTC was found to be better tolerated by patients than conventional colonoscopy. Furthermore, lesions were efficiently detected by CTC. We make efforts to improve both the sensitivity and specificity of CTC and hope to reduce the mortality rate of colon cancer with popularization of CTC