Preservation of Actin Immunoreactivity in Paraffin Section by Tannic Acid Fixation on Chick Embryonic Heart: Comparison with Phalloidin Staining on Frozen Section

Degree of binding reactivity to actin in variously fixed tissues was histochemically examined by using phalloidin and antiactin staining on paraffin or frozen sections of 5-day chick embryonic heart. Fluorescein-labeled phalloidin staining on frozen section of the specimens fixed with 4% formaldehyde showed intense staining of actin in the myocardium and the aorticopulmonary (AP) septum. However, phalloidin did not react to actin on paraffin sections of the specimens fixed with 4% formaldehyde, nor fixed with 4% formaldehyde and 1% tannic acid (TA). FITC-labeled antiactin staining on paraffin sections of the specimens fixed with 4% formaldehyde and 1% TA showed comparable fluorescent intensity with that of phalloidin staining on frozen sections. However antiactin staining on paraffin sections of the specimens fixed with 4% formaldehyde only showed reduction of fluorescent intensity around the Ap septum. Both phalloidin and antiactin react non-specifically to the tissues fixed with solutions including 1% glutaraldehyde. Since paraffin sections showed much better tissue preservation than frozen sections, we suggest that paraffin embedding with TA fixation is very useful for actin histochemistry using antiactin

Short Rib-Polydactyly Syndrome (Majewski Type): Report of a Case with Autopsy Findings

An autopsy case of short rib-polydactyly syndrome, type II (Majewski type) complicated by anisosplenia is reported, and some discussion has been made from the fetopathological viewpoint on the charactaristic findings and concomitant malformations of this syndrome with regard to a total of 23 cases, including our case and 22 cases reported in literature.Presented at the 24th Annual Meeting of the Congenital Anomalies Research Association of Japan July 6-7, 1984

市川市の働き盛り世代の生活習慣に関する研究 : 市川市受託研究活動報告

市川市の働き盛り世代(40～50歳代)の市民の健康診査と生活習慣アンケートを約4,000名を対象に実施した。アンケートは約2,000名の結果を回収し、健診とアンケートの結果について解析した。さらに、この結果に基づいて生活習慣病予防ための健康教育講座を計画し、アンケート回答者を対象に実施した。健診結果より生活習慣病のリスクを持っている者は男性に多かった。体調を表す不定愁訴スコアとBMI、家事負担感、食事満足度に関連性がみられた。栄養バランスを表す食品摂取頻度スコアは男性より女性で高く、BMIの高い肥満者では、スコアが低かった。運動習慣スコアは食品摂取スコア、食事満足度スコア、家事頻度スコアと関連性がみられ、運動習慣スコアが低い者では、BMIが高い者や高中性脂肪血症の者が多かった。健康教育講座について、食事、栄養、調理、運動に関する演習や実習を取り入れた少人数の講座は、参加した人は少なかったが有意義で効果的であった。以上の結果から、健診結果、体調、生活習慣(食事、運動)には多くの関連性があることについての根拠となる基礎資料が得られた。特に、今回用いた手法である体調や生活習慣に関するアンケート結果をスコア化して解析することが、これらの関連性を検討する方法として有効であることが示された

肝右葉切除の一症例における術後血清胆汁酸の変動について

Postoperative serum bile acids in a patient with hepatectomy were determined by gas-liquid chromatography. The results showed that two high peaks of serum bile acid levels were found respectively on the 2nd and 7th postoperative day, although serum bile acid levels returned to normal after the 15th postoperative day. The high peaks were considered to be due to the escape of necrotic hepatic intracellular bile acids into systemic blood stream, poor uptake of bile acids by hepatocytes and reabsorption of bile acids leaked from the residual liver into the peritoneal cavity

Urinary and Serum Bile Acids in Bile Peritonitis

Serum or urinary bile acids were determined in bile peritonitis with emphasis on peritoneal bile acid absorption. Bile peritonitis patients consisted of each two patients with insidious type, and acute or dramatic type, of bile peritonitis. All of them exhibited elevation in bile acid concentrations of 95.3, 37.0 mg/liter urine in the former, and of 87.6, 12.8 mg/liter serum in the latter. This finding implies that intraperitoneal bile acids were absorbed through the peritoneum into the blood resulting in hypercholanemia and hypercholanuria, in bile peritonitis. Early diagnosis of bile peritonitis is sometimes difficult because there are fewer signs of peritoneal irritation. Therefore our data indicate that enzymatic determination for serum bile acids is effective for early detection of bile peritonitis and thus the fatal outcome in insidious type of bile peritonitis can be avoided

ヒト胎児の免疫組織化学 : 胸腺と脾の Ig G, A, M 細胞

In order to study the development and differentiation of the immunological activity of human fetus, Ig G, A and M cells are demonstrated in the thymus and the spleen by means of Avidin Biotin Peroxidase Complex method. The results show (1) The Ig G cells appear in the thymus about 4 months of gastation and Ig A and M cells about 6 months of gestation in the thymus and the spleen in our human fetal specimens. (2) The Ig G cells are seen more often than Ig A and M cells both in the thymus and in the spleen. (3) Ig G, A and M cells are observed mainly in the medulla of the thymus and in the red pulp of the spleen mostly around the capillary vessels