N‑Doped Graphene Quantum Dots-Decorated V₂O₅ Nanosheet for Fluorescence Turn Off–On Detection of Cysteine

The development of a fast-response sensing technique for detection of cysteine can provide an analytical platform for prescreening of disease. Herein, we have developed a fluorescence turn off–on fluorescence sensing platform by combining nitrogen-doped graphene quantum dots (N-GQDs) with V₂O₅ nanosheets for the sensitive and selective detection of cysteine in human serum samples. V₂O₅ nanosheets with 2–4 layers are successfully synthesized via a simple and scalable liquid exfoliation method and then deposited with 2–8 nm of N-GQDs as the fluorescence turn off–on nanoprobe for effective detection of cysteine in human serum samples. The V₂O₅ nanosheets serve as both fluorescence quencher and cysteine recognizer in the sensing platform. The fluorescence intensity of N-GQDs with quantum yield of 0.34 can be quenched after attachment onto V₂O₅ nanosheets. The addition of cysteine triggers the reduction of V₂O₅ to V⁴⁺ as well as the release of N-GQDs within 4 min, resulting in the recovery of fluorescence intensity for the turn off–on detection of cysteine. The sensing platform exhibits a two-stage linear response to cysteine in the concentration range of 0.1–15 and 15–125 μM at pH 6.5, and the limit of detection is 50 nM. The fluorescence response of N-GQD@V₂O₅ exhibits high selectivity toward cysteine over other 22 electrolytes and biomolecules. Moreover, this promising platform is successfully applied in detection of cysteine in human serum samples with excellent recovery of (95 ± 3.8) – (108 ± 2.4)%. These results clearly demonstrate a newly developed redox reaction-based nanosensing platform using N-GQD@V₂O₅ nanocomposites as the sensing probe for cysteine-associated disease monitoring and diagnosis in biomedical applications, which can open an avenue for the development of high performance and robust sensing probes to detect organic metabolites