The Effect of Hydroalcoholic Extract of Glycyrriza Glabra Root on Anxiety in Gonadectomized Male Rats

Introduction: Anxiety is a common psychiatric disorder affecting many people in the society. Glycyrriza glabra is a herbal medicine, which carries a lot of traditional effects, this plant contains antioxidants and phytoestrogens. Phytoestrogens are plant sterols that are similar to estrogen in structure and function. The purpose of this study was to dinvestigate the effect of hydroalcoholic extract of Glycyrriza glabra root on anxiety in gonadectomized male rats. Methods: In this experimental study, 48 male rats with an average weight of 230-250g were used in the study. Rats were randomly divided into 6 groups. These groups included: healthy treatment group with saline , gonadectomized&nbsp; group with no treatment, gonadectomized group with treatment of saline or solvent extract (0.5 cc) and gonadectomized group with treatment doses of 2, 5 & 15 mg/kg Glycyrriza glabra root extract intraperitoneally (n=8). In a surgery, the testicles were removed. 30 minutes after injection. The Data were analyzed by one way variance analysis and Tukey&rsquo;s test using SPSS (p<0.05). Results: Anxiety was significantly increased in gonadectomy rats (P<0.001) when compared with the normal ones. At doses of 5 and 15 mg/kg/bw of hydro-alcholic extract of Glycyrriza glabra root, the time spent in open arms of maze were significantly increased and the time spent in maze close arms were decreased in compared to controls (P<0.05). None of the doses showed any significant effect on locomotor activites. Conclusion: Hydro-alcholic extract of Glycyrriza glabra root in rats not gonadectomized and Gonadectomy serve to reduce anxiety

Bilayer Amniotic Membrane/Nano-fibrous Fibroin Scaffold Promotes Differentiation Capability of Menstrual Blood Stem Cells into Keratinocyte-Like Cells

The skin provides a dynamic barrier separating and protecting human body from the exterior world, and then immediate repair and rebuilding of the epidermal barrier is crucial after wound and injury. Wound healing without scars and complete regeneration of skin tissue still remain as a clinical challenge. The demand to engineer scaffolds that actively promote regeneration of damaged areas of the skin has been increased. In this study, menstrual blood-derived stem cells (MenSCs) have been induced to differentiate into keratinocytes-like cells in the presence of human foreskin-derived keratinocytes on a bilayer scaffold based on amniotic membrane and silk fibroin. Based on the findings, newly differentiated keratinocytes from MenSCs successfully expressed the keratinocytes specific markers at both mRNA and protein levels judged by real-time PCR and immunostaining techniques, respectively. We could show that the differentiated cells over bilayer composite scaffolds express the keratinocytes specific markers at higher levels when compared with those cultured in conventional 2D culture system. Based on these findings, bilayer amniotic membrane/nano-fibrous fibroin scaffold represents an efficient natural construct with broad applicability to generate keratinocytes from MenSCs for stem cell-based skin wounds healing and regeneration. © 2017, Springer Science+Business Media, LLC, part of Springer Nature