Preventive Effect of Boiogito on Metabolic Disorders in the TSOD Mouse, a Model of Spontaneous Obese Type II Diabetes Mellitus

“Boiogito” is a Kampo preparation which has been used since ancient times in patients with obesity of the “asthenic constitution” type, so-called “watery obesity”, and its effect has been recognized clinically. In this study, we investigated the anti-obesity effect of Boiogito in the TSOD (Tsumura Suzuki Obese Diabetes) mouse, a model of spontaneous obese type II diabetes mellitus. Boiogito showed a significant anti-obesity effect in TSOD mice by suppressing body weight gain in a dosage-dependent manner. In addition, Boiogito showed significant ameliorative effects on features of metabolic syndrome such as hyperinsulinemia, fasting hyperglycemia and abnormal lipid metabolism. Regarding lipid accumulation in TSOD mice, Boiogito showed a significant suppressive effect on accumulation of subcutaneous fat, but the effect on the visceral fat accumulation that constitutes the basis of metabolic syndrome was weak, and the suppressive effect on insulin resistance was also weak. Furthermore, Boiogito did not alleviate the abnormal glucose tolerance, the hypertension or the peripheral neuropathy characteristically developed in the TSOD mice. In contrast, in the TSNO (Tsumura Suzuki Non-Obesity) mice used as controls, Boiogito suppressed body weight gain and accumulation of subcutaneous and visceral fat. The above results suggested that Boiogito is effective as an anti-obesity drug against obesity of the “asthenic constitution” type in which subcutaneous fat accumulates, but cannot be expected to exert a preventive effect against various symptoms of metabolic syndrome that are based on visceral fat accumulation

鉄欠乏性貧血における補完代替療法の適正使用に関する研究

取得学位：博士(薬学)，学位授与番号：博甲第629号，学位授与年月日：平成16年3月25日,学位授与年：200

Preventive Effects of Salacia reticulata on Obesity and Metabolic Disorders in TSOD Mice

The extracts of Salacia reticulata (Salacia extract), a plant that has been used for the treatment of early diabetes, rheumatism and gonorrhea in Ayurveda, have been shown to have an anti-obesity effect and suppress hyperglycemia. In this study, the effects of Salacia extract on various symptoms of metabolic disorder were investigated and compared using these TSOD mice and non-obese TSNO mice. Body weight, food intake, plasma biochemistry, visceral and subcutaneous fat (X-ray and CT), glucose tolerance, blood pressure and pain tolerance were measured, and histopathological examination of the liver was carried out. A significant dose-dependent decline in the gain in body weight, accumulation of visceral and subcutaneous fat and an improvement of abnormal glucose tolerance, hypertension and peripheral neuropathy were noticed in TSOD mice. In addition, hepatocellular swelling, fatty degeneration of hepatocytes, inflammatory cell infiltration and single-cell necrosis were observed on histopathological examination of the liver in TSOD mice. Salacia extract markedly improved these symptoms upon treatment. Based on the above results, it is concluded that Salacia extract has remarkable potential to prevent obesity and associated metabolic disorders including the development of metabolic syndrome

化学放射線療法を受ける頭頸部がん患者の皮膚バリア機能の変化 : ケーススタディ

目的：化学放射線療法を受ける頭頸部がん患者を対象とし、皮膚バリア機能および皮膚の客観的・主観的症状の変化を明らかにすることを目的とする。方法：3症例を対象とし、縦断的観察研究を行った。バリア機能の評価にはマイクロスコープ、皮膚の主観的症状はNRS、客観的症状はCTCAEを用いて評価した。結果：症例1）70代男性。照射量30.6Gyよりバリア機能の低下が認められ、同時期に疼痛が出現した。CTCAEは調査期間中グレード1で経過した。症例2）60代男性。照射量12.6Gyよりバリア機能の低下が認められ、23.4Gyで疼痛が出現した。CTCAEは調査期間中グレード1で経過した。症例3）50代男性。照射量30Gyで灼熱感が出現し、36Gyでバリア機能が低下するとともに掻痒感・疼痛も出現した。考察：肉眼的な皮膚障害は同程度であっても、バリア機能は患者によって差が生じていた。今後、肉眼ではとらえられない各患者のバリア機能の変化についてマイクロスコープを用いて評価することが可能である。Objective: To determine the changes in the skin barrier function and the objective and subjective symptoms of skin using microscopy in head and neck cancer patients undergoing chemoradiation therapy.Methods: A longitudinal observational study was conducted on three patients with head and neck cancer undergoing chemoradiation therapy. Microscopes were used to evaluate the skin barrier function and observe the skin texture. A numerical rating scale was used to assess the subjective skin symptoms such as pain, burning, and itching. The Common Terminology Criteria for Adverse Events（CTCAE）for radiation dermatitis was used to assess the objective symptoms.Results: In Case 1（a 70-year-old man）, grade 1 CTCAE was observed throughout the study period. In Case 2（a 60-year-old man）, a decline in the barrier function was observed after a total radiation dose of 12.6 Gy. Grade 1 CTCAE was observed during the study period. In Case 3（a 50-year-old man）, the barrier function decreased and burning, itching, and pain sensations were reported at a dose of 36 Gy. Grade 3 CTCAE was observed at a total dose of 57.6 Gy.Discussion: This study shows that although the skin damage appears similar on evaluation by the naked eye, the skin barrier functions may differ among patients. In the future, the use of microscopes could help to evaluate the changes in the barrier function that cannot be observed by the naked eye