Dodin fungusitinin Vicia faba L. bitkisinde total protein miktarı ve peroksidaz aktivitesi üzerine etkisi

Bu araştırmada dodin fungusitinin Vicia faba L. fizyolojisi üzerindeki etkisi, total protein miktarı ve peroksidaz (POX) aktivitesi aracılığıyla değerlendirilmiştir. Bu doğrultuda, dört haftalık V. faba fideleri 0.04 mL/L, 0.08 mL/L ve 0.16 mL/L'lik dodin konsantrasyonları ile muamele edilmiştir. Bu işlemlerden sonra bitki örneklerinin yaprakları 24, 48 ve 72 saat ara ile hasat edilmiştir. Yapılan analizler sonucunda, artan dodin konsantrasyonu ve muamele süresiyle total protein miktarı kontrole göre önemli ölçüde azalırken, peroksidaz aktivitesi yükselmiştir. Elde edilen sonuçlar dodinin bitki savunma sistemini uyardığını göstermiştir.This research was to evaluate the effects of dodine on the physiology of Vicia faba L. through total protein content and peroxidase (POX) activity. According to this, four weeks old seedlings were treated with 0.04 mL/L, 0.08 mL/L and 0.16 mL/L concentrations of dodine. Then, the leaves of plant samples were harvested with 24, 48 and 72 hours intervals. Our results have showed that while the total protein content were decreased significantly, POX activity was increased with the increased concentration of dodine and exposure time when compared with control seedlings. This research have shown that dodine can stimulate the plant defense system

Psoriasis and Genetics

Psoriasis is an erythematous, scaly chronic inflammatory dermatosis and occurs due to altered epidermal differentiation and hyperproliferation due to faulty signals that speed up the growth cycle of skin cells. Psoriasis reduces quality of life, and psoriatic patients generally have higher risk for metabolic disease. Psoriasis is associated with many burdening comorbidities, which often share similar pathogenic features and follow a progressive pattern. Genetic variation in human genome causes specific kind of disease, and nowadays, research is focused on metabolic pathways that trigger psoriasis and related comorbidities. In addition, genetic variations are also important for psoriasis treatment regime and response. The purpose of this section is to shown to genetic epidemiology, pharmacogenetics, immune genetics of psoriasis and related comorbidities

Capsicum annuum L. 'un bazı varyeteleri üzerinde doku kültürü çalışmaları

Bu tezin, veri tabanı üzerinden yayınlanma izni bulunmamaktadır. Yayınlanma izni olmayan tezlerin basılı kopyalarına Üniversite kütüphaneniz aracılığıyla (TÜBESS üzerinden) erişebilirsiniz.V ABSTRACT IN VITRO STUDIES ON SOME VARIETIES OF CAPSICUM ANNUUM L. AKI, CÜNEYT Ph Ü Thesis in Biology Department Supervisor: Prof.Dr. M. Giinnehir O?UZ March 1997, 82 pages In this investigation, in vitro studies was made on the Capsicum annuum varieties that is very important crop for Turkey. During the in vitro studies, M.S. (Murashige-Skoog) medium was supplemented with various kind and combinations of auxines and cytokinins which are very useful for in vitro studies like plant growth regulators. In this study, we excised the hypocotyl, cotyledone segments from aseptically grown three weeks old seedling of pepper varieties. This seedlings grown from seeds which surfaced sterilized with % 70 ethanol for 4 min % 10 NaOCl (commercial bleach) solution for 6 min. The seeds were sown on the surface of hormone free M.S. (Murashige-Skoog) medium, ph adjusted to 5.7-5.8, the medium was solidified with % 0.7 of bactoagar and % 2 of sucrose used for carbon and energy source. After that, the cultures of the seeds germinated under 16 h. photoperiod at 25°C. Cotyledone and hypocotyl explants of 3 weeks old seedlings were excised in approximately 1 cm. length segments. These explants were cultured on M.S. basal medium supplemented with different concentrations and combinations of the different plant growth regulators like auxines (IAA:NAA) and cytokinins ( BAP: KIN ). We used this plant growth regulators in 1, 5, 10 mg/1 dozes for supplement the M.S. medium and most effective concentration for callus initation from explants was found 5 mg/1 for some of the cultivars which we get to the culture. Combination of auxines and cytokinin 1:2 was found most effective dozes for callus formation in all cultivars. In addition that, when we finished this series, we determined to find drought stress or lowering osmotic potential conditions in the medium, what were the effects on the callus formation and response of the biomass induction. For this reason, we added commonly PEG 3350 in % 10 concentration to the M.S. medium. PEG is a reason for water stress and lovering osmotic potential conditions in the medium which there are callus portion. After addition % 10 PEG to the medium, we get the measurement of biomass with 3 weeks interval from the callus. Keywords: Capsicum annuum L., auxines, cytokinins, PEG, callusIV ÖZET CAPSICUM ANNUUM L.' UN BAZI VARYETELERİ ÜZERİNDE DOKU KÜLTÜRÜ ÇALIŞMALARI AKI,Cüneyt Doktora Tezi, Biyoloji Bölümü Tez Yöneticisi: Prof.Dr. M.Günnehir O?UZ Mart 1997, 82 sayfa Çalışmamızda ülkemiz açısından ekonomik önemi bulunan Capsicum annuum L. 'un, bazı varyeteleri üzerinde in vitro çalışmalar gerçekleştirilmiştir. Denemelerimizde kullanılacak bitkisel materyaller in vitro koşullarda yetiştirilmiştir. Bu amaç ile C annuum varyetelerine ait olan tıhumlar % 70' lik Etanolde 4 dk., % 10' luk Hipoklorit içerisinde 6 dk. tutularak sterilizasyonları gerçekleştirilmiştir. Daha sonra pH' ı 5.7-5.8 olarak ayarlanan, % 0.7' lik agar ile katılaştırılmış, % 2 sakkaroz eklenen hormonsuz M.S. ortamlarına ekilen tohumlar, iklim odasına alınmıştır. 3 haftalık fıdeciklere ait olan hipokotil ve kotiledon eksplantları kesilerek, bitki büyüme düzenleyicilerinden NAA, IAA, BAP ve KIN' in 1-5-10 mg.l1 konsantrasyonları ve NAA:BAP, IAA:BAP ve NAA:KIN, IAAıKIN1 in 1:2 mg/1' ve 1:4 mg/T lik kombinasyonları ile desteklenen M.S. ortamlarında kültüre alınmışlardır.Denemelerimizde M.S. ortamına eklenen oksinlerden 5 mg/1 NAA ve IAA, 5 mg/1 KIN ve BAP kallus oluşumunu uyarmada en etkili konsantrasyon olarak saptanmıştır. Daha düşük veya yüksek dozların kültüvarlar arasında farklı tepkiler verdiği ortaya koyulmuştur. Kullandığımız oksin (NAA ve IAA) : sitokinin (KIN ve BAP) kombinasyonlarından kallus oluşumu için en etkili ortamların oksin sitokinin oranın 1:2 olarak eklendiği M.S. ortamları olduğu gözlenmiştir. Bu kombinasyonlarda da kültüvarlar arası farklılıklar göze çarpmaktadır. Denemelerimizin diğer bölümünde ise kallus verimi yüksek bulunan kültüvarlardan Kandil Dolma ve Yağlık 28' in osmotik basıncı % 10 PEG eklenerek değiştirilmiş M.S. ortamlarındaki canlı kütle değişimi üzerinde oksin ve sitokininlerin birlikte ve tek başlarına etkilerini araştırılmıştır. Bu şekilde iki kültüvardaki canlı kütle değişimleri arasındaki farklılıklar ortaya konmuştur

Heavy Metal Biosorption and Plasmid Profiles of Heavy Metal Resistance Bacteria isolated from Soil Samples of Thermal power, Canakkale, Turkey

WOS: 000408242800005The aim of this study is to investigate the isolation, characterization, determination of plasmid contents and metal absorption capacities of heavy metal-resistant bacteria from soil samples of thermal power. Samples were collected from soil in the area of thermal power. To define the resistance of heavy metal, metal solutions of ZnCl2, FeCl3 6 center dot H2O, Pb(NO3)(2) and CuSO4 in varying concentrations were added to culture media by using MIC method. The bacteria of highest MIC level were defined through VITEK 2 system and their plasmid profiles were determined. The metal biosorption capacities of two isolates were defined through ICP-AES. In total, 37 heavy metal resistant bacteria were isolated from soil samples. Results showed that 15 of them contained plasmid DNA. Finally, biosorption experiments with two isolates with the highest metal resistance were performed. While the metal having the highest growing speed of biosorption among Fe, Zn, Pb and Cu metals which were used in this study for Bacillus mycoides (isolate 26) is Zn, it is followed by Pb, Fe and Cu orderly. While the metal having the highest growing speed of biosorption for Enterobacter cloacea complex (isolate 10) is Pb, it is followed by Zn, Fe and Cu orderly.Canakkale Onsekiz Mart UniversityCanakkale Onsekiz Mart University [BAP- 2011/116]This investigation is a part of Ph. D. thesis. This work was supported by the Research Fund of Canakkale Onsekiz Mart University. (Project number BAP- 2011/116)

Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids

Due to the increasing demand and over collection of orchids from nature to produce salep, scientists have been led to search for more efficient ways to propagate these specific orchids in vitro. This present study compares germination performances of two commercial (Orchimax and Knudson C) and one specially prepared orchid growth media (SV), on economically and medicinally important orchids used to make salep; (Anacamptis pyramidalis, Dactylorhiza romana, Neotinea tridentata and Anacamptis morio subsp. morio) and further aims to obtain a mature orchid plant by following the natural environmental cycle. Significant differences in seed germination and protocorm development were observed. Asymbiotic germination tests showed that the specially prepared growth media performed better than the commercial media by 79.11% germination rate. Also, that A. morio subsp. morio had the best germination rate by 88.91%. Protocorms developed in the sixteenth week after sowing. Soil was collected from the natural habitat of each species and was used as a potting substrate, and this helped orchids to pass their initial acclimatization stage. Regeneration success of orchids at in vitro conditions could be increased by using SV growth medium, following their natural hardening off cycle and using specific substrates from their respective habitats

Dodin fungusitinin Vicia faba L. bitkisinde total protein miktarı ve peroksidaz aktivitesi üzerine etkisi

Bu araştırmada dodin fungusitinin Vicia faba L. fizyolojisi üzerindeki etkisi, total protein miktarı ve peroksidaz (POX) aktivitesi aracılığıyla değerlendirilmiştir. Bu doğrultuda, dört haftalık V. faba fideleri 0.04 mL/L, 0.08 mL/L ve 0.16 mL/L’lik dodin konsantrasyonları ile muamele edilmiştir. Bu işlemlerden sonra bitki örneklerinin yaprakları 24, 48 ve 72 saat ara ile hasat edilmiştir. Yapılan analizler sonucunda, artan dodin konsantrasyonu ve muamele süresiyle total protein miktarı kontrole göre önemli ölçüde azalırken, peroksidaz aktivitesi yükselmiştir. Elde edilen sonuçlar dodinin bitki savunma sistemini uyardığını göstermiştir

Evaluation of genotoxicity and cytotoxicity of dodine (1-dodecylguanidium acetate) by Allium test

In this study, we evaluated the genotoxic and cytotoxic effects of dodine, a fungicide extensively used to control scab on apples, pears and pecans, brown rot on peaches and several foliar diseases of cherries, strawberries, peaches and black walnuts. For this purpose the Allium cepa test was carried out exposing roots to dodine for 24,48 and 72 h at the concentrations of EC50/2, EC50 and 2×EC50. The mitotic index was calculated as the number of dividing cells per number of 3000-4000 observed cells and the mitotic aberrations also were scored at each concentration. The results showed that dodine induced significant increases of mitotic aberrations such as C-mitosis, polar shifting, laggard chromosome and chromosome fragments. In addition, mitotic index decreased significantly with increasing of concentration and the exposure time as compared to their controls. Hence dodine should be used under control in agricultural fields due to its possible toxic effects. © by PSP

Domatesin bakteriyel hastalıklarının kontrolünde bitki aktivatörleri ve bakterisitlerin etkileri

In this study, we aimed to integrated plant activators as systemic acquired resistance (SAR) in to available chemical control measures, consequently to get comparable efficacy withless chemical. Variations in the activity of. specific peroxidase enzyme that likely represent the enhancement of host resistance were analyzed from the leaves of tomato seedlings after individual and combined applications. The efficacy tests of the compounds against the major diseases of tomato were conducted with pot experiments under controlled conditions in greenhouse. Possible correlations between higher efficacy of the compound in question and increased specific enzyme activity were evaluated with SPSS 8.0 for Windows. The highest efficacy for the control of both bacterial spot (Pseudomonas syringae pv. tomato) and bacterial speck (Xanthomonas campestris pv vesicatoria), and the highest specific enzyme activities were obtained from Cupracol applications with about 78% and 140%, and 70% and 122%, respectively. The results have revealed that comparable efficacies with combined applications of SAR inducers with suitable bactericides and fungicides-could be likely acceptable and applicable in practice.Bu çalışmada, kullanılan kimyasal kontrol yöntemlerine, sistemik uyarılmış dayanıklılık olarak (SAR) bitki aktivatörlerinin entegre edilmesi ve sonuçta daha az kimyasalla kabul edilebilir etkililik elde edilmesi amaçlanmıştır. Muhtemelen konukçu dayanıklılığındaki artışı gösteren spesifik peroksidaz enzim aktivitesihdeki değişimler, tek tek ve kombine edilmiş uygulamalardan sonra domates fidesi yapraklarından analiz edilmiştir. Domatesin gerek bakteriyel benek (Pseudomonas syringae pv. tomato) ve gerekse bakteriyel leke (Xanthomonas campestris pv vesicatoria) hastalıklarına karşı söz konusu. bileşiklerin etkililik testleri serada, kontrollü koşullar altında, saksı denemeleri ile gerçekleştirilmiştir. Bu bileşiklerin yüksek etkililiği ile artan peroksidaz enzim aktivitesi arasındaki olası ilişkiler, karşılaştırmalı olarak SPSS 8.0 for Windows, programı ile değerlendirilmiştir. Bakteriyel benek (Pseudomonas syringae pv. tomato) ve bakteriyel leke (Xanthomonas campestris pv vesicatoria) hastalıklarının kontrolü için en iyi etkililik ve en yüksek spesifik enzim aktivitesi sırasıyla % 78 ve % 140 ve % 70 ve % 122 oran ile Cupracol uygulamalarından elde edilmiştir. Diğer sonuçlar ise uygun bakterisit ve fungisitler ile SAR uyarıcılarının kombine uygulamaları sonucunda karşılaştırılabilir etkililiğin pratikte kabul edilebilirliğini ve uygulanabilirliğini göstermektedir