Genetic conservation through effective utilization of the improved indigenous chicken breeds by rural households in Nigeria

SUMMARY. Our collection and genetic characterization of various indigenous chicken genotypes in Nigeria started in 1984 at the National Animal Production Research Institute, Shika, Zaria, (Shika Brown) in 1994 at the Federal University of Agriculture, Abeokuta, Nigeria (FUNAAB) and in 2014 in Obafemi Awolowo University, Ile-Ife (FULANI). Collections were screened and characterized for genetic improvement and effective utilization by rural households in Nigeria With the financial and technical support of the Bill and Melinda Gates Foundation and the International Livestock Research Institute, Ethiopia, respectively, improved chicken genotypes were developed and evaluated alongside some other tropically adapted chicken breeds. A total of six improved tropically adapted chicken breeds (three Nigerian – Shika Brown, FUNAAB alpha and Fulani; three imported – Kuroiler, Sasso and Noiler) were tested for 52 weeks on-farm, across five agro-ecological zones of Nigeria and on-station in a public University farm and a private farm in Ogun and Oyo States, respectively. Results showed the Nigeria’s improved Indigenous chicken breed, the FUNAAB-Alpha, to be of comparable performance with the three imported breeds. It has met with high acceptance and demand after exhibition of the six breeds during the 2017 Science and Technology Expo in Abuja, Nigeria

Emergence and spread of two SARS-CoV-2 variants of interest in Nigeria.

Identifying the dissemination patterns and impacts of a virus of economic or health importance during a pandemic is crucial, as it informs the public on policies for containment in order to reduce the spread of the virus. In this study, we integrated genomic and travel data to investigate the emergence and spread of the SARS-CoV-2 B.1.1.318 and B.1.525 (Eta) variants of interest in Nigeria and the wider Africa region. By integrating travel data and phylogeographic reconstructions, we find that these two variants that arose during the second wave in Nigeria emerged from within Africa, with the B.1.525 from Nigeria, and then spread to other parts of the world. Data from this study show how regional connectivity of Nigeria drove the spread of these variants of interest to surrounding countries and those connected by air-traffic. Our findings demonstrate the power of genomic analysis when combined with mobility and epidemiological data to identify the drivers of transmission, as bidirectional transmission within and between African nations are grossly underestimated as seen in our import risk index estimates

The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance

INTRODUCTION Investment in Africa over the past year with regard to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing has led to a massive increase in the number of sequences, which, to date, exceeds 100,000 sequences generated to track the pandemic on the continent. These sequences have profoundly affected how public health officials in Africa have navigated the COVID-19 pandemic. RATIONALE We demonstrate how the first 100,000 SARS-CoV-2 sequences from Africa have helped monitor the epidemic on the continent, how genomic surveillance expanded over the course of the pandemic, and how we adapted our sequencing methods to deal with an evolving virus. Finally, we also examine how viral lineages have spread across the continent in a phylogeographic framework to gain insights into the underlying temporal and spatial transmission dynamics for several variants of concern (VOCs). RESULTS Our results indicate that the number of countries in Africa that can sequence the virus within their own borders is growing and that this is coupled with a shorter turnaround time from the time of sampling to sequence submission. Ongoing evolution necessitated the continual updating of primer sets, and, as a result, eight primer sets were designed in tandem with viral evolution and used to ensure effective sequencing of the virus. The pandemic unfolded through multiple waves of infection that were each driven by distinct genetic lineages, with B.1-like ancestral strains associated with the first pandemic wave of infections in 2020. Successive waves on the continent were fueled by different VOCs, with Alpha and Beta cocirculating in distinct spatial patterns during the second wave and Delta and Omicron affecting the whole continent during the third and fourth waves, respectively. Phylogeographic reconstruction points toward distinct differences in viral importation and exportation patterns associated with the Alpha, Beta, Delta, and Omicron variants and subvariants, when considering both Africa versus the rest of the world and viral dissemination within the continent. Our epidemiological and phylogenetic inferences therefore underscore the heterogeneous nature of the pandemic on the continent and highlight key insights and challenges, for instance, recognizing the limitations of low testing proportions. We also highlight the early warning capacity that genomic surveillance in Africa has had for the rest of the world with the detection of new lineages and variants, the most recent being the characterization of various Omicron subvariants. CONCLUSION Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve. This is important not only to help combat SARS-CoV-2 on the continent but also because it can be used as a platform to help address the many emerging and reemerging infectious disease threats in Africa. In particular, capacity building for local sequencing within countries or within the continent should be prioritized because this is generally associated with shorter turnaround times, providing the most benefit to local public health authorities tasked with pandemic response and mitigation and allowing for the fastest reaction to localized outbreaks. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century

Proteinaceous diet inhibits gossypol-induced spermatotoxicity

The present work was designed to investigate the effect of a proteinous dietary supplement, fishmeal, on gossypol – induced spermatotoxicity. Twenty-five adult male Wistar rats, averaging 205g, were randomly sorted into four experimental groups (I – IV) of 5 animals each, and a control group. Crude cottonseed oil was administered orally to each animal in groups I – IV at a rate that provided 14mg/kg/d free gossypol; besides, 3g/d, 7g/d, and 10g/d of fishmeal was provided as meal supplement to each animal in groups I, II and III respectively. The control group received rat pellets and water freely. At the end of the 53 – day treatment period, all animals were placed under chloroform anaesthesia; the caudal epididymides were removed, minced and placed in Ham’s F10 solution for the evaluation of sperm count and motility. The testes were also processed for histological studies using the eosin and haemetoxylin (H & E) method. Our findings revealed a dose – dependent inhibition of gossypol – induced spermatotoxicity by the supplemented fishmeal; this suggests that proteinous diets are protective against gossypol – induced male infertility

Asymptomatic Bacteriuria in Antenatal Patients in Ilorin, Nigeria

 Objective: To determine the prevalence of asymptomaticbacteriuria, bacteriology and sensitivity pattern in Ilorin using thegold standard of urine culture.Methods: A prospective study was carried out from 1st Julyto 31st October 2007, at the University of Ilorin TeachingHospital (UITH) on 125 consenting asymptomatic pregnantwomen. A structured proforma was used to collect informationfrom the women and a midstream urine specimen collected forbacteriological culture.Results: Of the 125 pregnant women, 50 had bacteriuria on urineculture giving a prevalence of 40�20The mean age of the womenwas 28.5 years with a standard deviation of 4.95. The age rangedbetween 14 and 40 years. Staphylococcus aureus was the commonestpathogen isolated (72� followed by Proteus spp (14� Most ofthe organisms showed good sensitivity to Nitrofurantoin andgentamicin.Conclusion: The prevalence of asymptomatic bacteriuria in Ilorinis high and routine urine culture is advocated for all pregnantwomen at booking