401 research outputs found
Covalent labelling of ligand binding sites of human placental S-adenosylhomocysteine hydrolase with 8-azido derivatives of adenosine and cyclic AMP
Selective demethylation of the 5-methoxyl group in flavanones and synthesis of dihydrowogonin
This article does not have an abstract
Study of strong photon-magnon coupling in a YIG-film split-ring resonant system
By using the stripline Microwave Vector Network Analyzer Ferromagnetic
Resonance and Pulsed Inductive Microwave Magnetometry spectroscopy techniques,
we study a strong coupling regime of magnons to microwave photons in the planar
geometry of a lithographically formed split-ring resonator (SRR) loaded by a
single-crystal epitaxial yttrium-iron garnet (YIG) film. Strong anti-crossing
of the photon modes of SRR and of the magnon modes of the YIG film is observed
in the applied-magnetic-field resolved measurements. The coupling strength
extracted from the experimental data reaches 9 percent at 3 GHz.
Theoretically, we propose an equivalent circuit model of an SRR loaded by a
magnetic film. This model follows from the results of our numerical simulations
of the microwave field structure of the SRR and of the magnetization dynamics
in the YIG film driven by the microwave currents in the SRR. The equivalent
circuit model is in good agreement with the experiment. It provides a simple
physical explanation of the process of mode anti-crossing.
Our findings are important for future applications in microwave quantum
photonic devices as well as in magnetically tunable metamaterials exploiting
the strong coupling of magnons to microwave photons
Can Europe recover without credit?
Data from 135 countries covering five decades suggests that creditless recoveries, in which
the stock of real credit does not return to the pre-crisis level for three years after the GDP
trough, are not rare and are characterised by remarkable real GDP growth rates: 4.7 percent
per year in middle-income countries and 3.2 percent per year in high-income countries.
However, the implications of these historical episodes for the current European situation are
limited, for two main reasons. First, creditless recoveries are much less common in highincome
countries, than in low-income countries which are financially undeveloped. European
economies heavily depend on bank loans and research suggests that loan supply played a
major role in the recent weak credit performance of Europe. There are reasons to believe that,
despite various efforts, normal lending has not yet been restored. Limited loan supply could
be disruptive for the European economic recovery and there has been only a minor
substitution of bank loans with debt securities. Second, creditless recoveries were associated
with significant real exchange rate depreciation, which has hardly occurred so far in most of
Europe. This stylised fact suggests that it might be difficult to re-establish economic growth
in the absence of sizeable real exchange rate depreciation, if credit growth does not return
S-Adenosylhomocysteine hydrolase from human placenta. Affinity purification and characterization
Stimulation of intracellular topoisomerase I activity by vasopressin and thrombin. Differential regulation by pertussis toxin
Induction of c-fos protein by activation of vasopressin receptors in smooth muscle cells
AbstractStimulation of vasopressin (V1) receptors of rat aortic smooth muscle cells (A-10, ATCC CRL 1476) results in the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) with the mobilization of intracellular calcium. When A-10 cells are exposed to arginine vasopressin (AVP), there is an increase in the level of c-fos oncoprotein. The extent of induction of c-fos oncoprotein depends on both the time of exposure of the cells to AVP, reaching a maximum at 60 min after which there is a slow decline, and the concentration of AVP used, with an approximate EC50 of 1 nM which corresponds well with the Kd of vasopressin binding to these receptors. This vasopressin-mediated increase in c-fos protein level is inhibited by a V1/V2 antagonist (SKF 101498) suggesting that this is a receptor-mediated event. In addition dDAVP, a V2 selective agonist, is much less effective than AVP in inducing c-fos protein suggesting that AVP mediates its effect via V1 receptors. Desensitization of vasopressin receptors by prolonged exposure to AVP resulted in no additional induction of c-fos protein level in response to second challenge of AVP. In addition to AVP, phorbol dibutyrate (PDBu), an activator of protein kinase C (PKC), also stimulates the accumulation of c-fos protein although to a lesser extent than AVP. The above data suggest that c-fos protein levels in smooth muscle cells are regulated by AVP and the hormonal effect may be mediated through PI turnover and DAG, IP3 and Ca2+ signals
Localisation of NMU1R and NMU2R in human and rat central nervous system and effects of neuromedin-U following central administration in rats
Rationale: Neuromedin-U (NmU) is an agonist at NMU1R and NMU2R. The brain distribution of NmU and its receptors, in particular NMU2R, suggests widespread central roles for NmU. In agreement, centrally administered NmU affects feeding behaviour, energy expenditure and pituitary output. Further central nervous system (CNS) roles for NmU warrant investigation.
Objectives: To investigate the CNS role of NmU by mapping NMU1R and NMU2R mRNA and measuring the behavioural, endocrine, neurochemical and c-fos response to intracerebroventricular (i.c.v.) NmU. Methods: Binding affinity and functional potency of rat NmU was determined at human NMU1R and NMU2R. Expression of NMU1R and NMU2R mRNA in rat and human tissue was determined using semi-quantitative reverse-transcription polymerase chain reaction. In in-vivo studies, NmU was administered i.c.v. to male Sprague-Dawley rats, and changes in grooming, motor activity and pre-pulse inhibition (PPI) were assessed. In further studies, plasma endocrine hormones, [DOPAC + HVA]/[dopamine] and [5-HIAA]/[5-HT] ratios and levels of Fos-like immunoreactivity (FLI) were measured 20 min post-NmU (i.c.v.).
Results: NmU bound to NMU1R (KI, 0.11±0.02 nM) and NMU2R (KI, 0.21±0.05 nM) with equal affinity and was equally active at NMU1R (EC50, 1.25±0.05 nM) and NMU2R (EC50, 1.10±0.20 nM) in a functional assay. NMU2R mRNA expression was found at the highest levels in the CNS regions of both rat and human tissues. NMU1R mRNA expression was restricted to the periphery of both species with the exception of the rat amygdala. NmU caused a marked increase in grooming and motor activity but did not affect PPI. Further, NmU decreased plasma prolactin but did not affect levels of corticosterone, luteinising hormone or thyroid stimulating hormone. NmU elevated levels of 5-HT in the frontal cortex and hypothalamus, with decreased levels of its metabolites in the hippocampus and hypothalamus, but did not affect dopamine function. NmU markedly increased FLI in the nucleus accumbens, frontal cortex and central amygdala.
Conclusions: These data provide further evidence for widespread roles for NmU and its receptors in the brain
Identification of insect-specific target genes for development of RNAi based control of the Eucalyptus gall pest Leptocybe invasa Fisher & La Salle (Hymenoptera: Eulophidae)
Mouse Cofactor of BRCA1 (Cobra1) Is Required for Early Embryogenesis
Negative elongation factor (NELF) is a four-subunit protein complex conserved from Drosophila to humans. In vitro biochemical and tissue culture-based studies have demonstrated an important role of NELF in controlling RNA polymerase II (Pol II) pausing in transcription. However, the physiological significance of NELF function is not clear due to the lack of any genetic systems for studying NELF.Here we show that disruption of the mouse B subunit of NELF (NELF-B), also known as cofactor of BRCA1 (Cobra1), causes inner cell mass (ICM) deficiency and embryonic lethality at the time of implantation. Consistent with the phenotype of the Cobra1 knockout (KO) embryos, knockdown of Cobra1 in mouse embryonic stem cells (ESCs) reduces the efficiency of colony formation and increases spontaneous differentiation. Cobra1-depleted ESCs maintain normal levels of Oct4, Nanog, and Sox2, master regulators of pluripotency in ESCs. However, knockdown of Cobra1 leads to precocious expression of developmental regulators including lymphoid enhancer-binding factor 1 (Lef1). Chromatin immunoprecipitation (ChIP) indicates that Cobra1 binds to the Lef1 promoter and modulates the abundance of promoter-bound RNA polymerase.Cobra1 is essential for early embryogenesis. Our findings also indicate that Cobra1 helps maintain the undifferentiated state of mESCs by preventing unscheduled expression of developmental genes
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