Zebrafish MCH receptor mRNA expression and regulation by intestinal inflammation.

<div><p>A) Relative expression of zebrafish MCHR1b and MCHR2 in brain and the intestine.</p> <p>B) Expression of intestinal MCHR1b and MCHR2 mRNA following treatment with TNBS or vehicle (n=9-10 fish per group). Values in the vehicle treatment group are set to 100.</p> <p>AU: arbitrary units.</p></div

Vancomycin administration reduces mortality associated to TNBS-enterocolitis.

<div><p>A) Graphical representation of the study design. Vancomycin (100mg/L) was added to the fish tank water 18 hours prior to the induction of colitis.</p> <p>B) Kaplan-Meier analysis comparing the effect of treatment with vancomycin or vehicle on the survival of zebrafish with TNBS enterocolitis (n=21-25 fish per group).</p></div

Adult zebrafish are susceptible to TNBS-induced enterocolitis.

<p>Kaplan-Meier survival analysis in response to different concentrations of TNBS (40mM-320mM). TNBS was administered by intrarectal infusion and doses were adjusted for body weight to a volume of 1 uL/0.1 g of body weight. Fish were monitored for a total of 96 hours in 6-12 hour intervals. For each TNBS dose, 13-14 fish per group were assigned to TNBS or vehicle (30% ethanol) treatments. </p

Relative mRNA expression of various cytokines in the intestine of zebrafish with TNBS-induced enterocolitis, at 6 hours post-exposure (n=9-10 fish per group).

<p>For each gene, expression in the vehicle treated group was set to 100. AU=arbitrary units.</p

MCH expression in the adult zebrafish intestine.

<div><p>A) Amino acid sequence alignments of the MCH peptides from human, mouse and zebrafish. Identical residues are highlighted.</p> <p>B) Comparative mRNA expression of the zebrafish MCH peptides in brain and the gastrointestinal tube, presented in logarithmic scale. (n=8-9 fish per group).</p></div

Sequence alignment of MCH receptors in various species.

<div><p>A) Sequence alignment of zebrafish MCHR1a, MCHR1b and human and mouse MCHR1.</p> <p>B) Sequence alignment of MCHR2 receptors in zebrafish, human and mouse .</p> <p>Identical residues are highlighted.</p> <p>C) Graphical representation of sequence identity for MCH receptors among species.</p></div