Table_1_Predictive value of ApoB/ApoA-I for recurrence within 1 year after first incident stroke.DOCX

BackgroundApoB/ApoA-I ratio is a reliable indicator of cholesterol balance, particularly in the prediction of ischemic events risk. The aim of this study was to investigate the prognostic value of ApoB/ApoA-I for stroke recurrence within 1 year after the first incident.MethodsWe retrospectively included patients who were first diagnosed with acute (ResultsA total of 722 patients with acute ischemic stroke were included, of whom 102 experienced stroke recurrence within 1 year, with a recurrence rate of 14.1%. Serum ApoB/ApoA-I concentrations on admission were higher in patients with stroke recurrence at 1 year compared with those with a good prognosis (P DiscussionApoB/ApoA-I ratio, measured during the acute phase of the first stroke, was positively correlated with the risk of stroke recurrence within 1 year.</p

Video_1_Case report: Varicella-zoster virus infection triggering progressive encephalomyelitis with rigidity and myoclonus.MP4

Progressive encephalomyelitis with rigidity and myoclonus (PERM) is a rare neurological disease of unknown etiology, and most patients with PERM are positive for anti-glycine receptor (GlyR) antibody. In this case study, we report a clinical case of a varicella-zoster virus-infected patient who developed anti-GlyR antibody-positive PERM. He initially suffered from herpes zoster and gradually developed symptoms of impaired brainstem functions including hoarse voice and dysphagia, accompanied by paroxysmal sympathetic hyperactivity. The patient also suffered from severe spasms, which were easily triggered by external stimuli. Glycine receptor antibodies were then found to be positive in serum and cerebrospinal fluid, and the diagnosis of PERM was confirmed. Methylprednisolone and gamma globulin treatments were given, and spasms were improved after treatment. Unfortunately, the patient's family insisted on automatic discharge and the patient passed away several days later.</p

Different developmental stages of goat embryos produced <i>in vitro</i>.

<p>Transferred IVF blastocysts (a) or SCNT blastocysts (b) showed clear inner cell masses (ICMs) and trphoectodermal cells (TE) under bright field at Day 7. Both the IVF blastocysts (c) and SCNT blastocysts (d) attached to the endometrial epithelial cells after co-cultured for 24 h. The trophoblast of IVF (e) and SCNT (f) underwent outgrowth on the epithelial cells monolayer after co-cultured for 72 h. Scale bar = 50 µm.</p

Characterization of primary endometrial epithelial cells (EECs) and endometrial stromal cells (ESCs).

<p>The cobblestone structure and expression of cytokeratin were shown in EECs (a, b) and the spindle-shape structure and vimentin expression were shown in ESCs (e, f). The negative control shown of cytokeratin and vimentin involved use of mouse IgG in place of primary antibody (c, g). Western blot analysis of estrogen receptor (ER) and progesterone receptor (PR) protein expression were shown separately in the endometrial cells (d, h). Results were quantified by densitometry analysis of the bands and normalization to β-actin protein. Data represent the mean ± standard error of the mean (SEM) from five independent experiments. Columns with different superscript letters are significantly different (<i>P</i> < 0.05). Scale bar = 30 µm.</p

Measurement of cell apoptosis by FACS at 48 h post transfection with the shLuman lentivirus and the shRNA-negative lentivirus (mean ± SEM, n = 3).

<p>Measurement of cell apoptosis by FACS at 48 h post transfection with the shLuman lentivirus and the shRNA-negative lentivirus (mean ± SEM, n = 3).</p

Effects of Luman knockdown on the mRNA expression levels of genes related to folliculogenesis and luteinization.

<p>(A) The mRNA expression level of <i>Has2</i> was decreased, and (B) the mRNA expression level of <i>Ptgs2</i> was increased in the shLuman group compared with the shRNA-negative group. The levels of mRNA were normalized to that of <i>β-actin</i>. Values are presented as the mean ± SEM, n = 3. Asterisks indicate significant differences (P < 0.05).</p

Effects of Luman knockdown on cell cycle.

<p>(A~D) The mRNA expression levels of the indicated cell cycle-related genes (<i>Cyclin A1</i>, <i>Cyclin B1</i>, <i>Cyclin D2</i> and <i>Cyclin E</i>) were increased in the shLuman group compared with the shRNA-negative group. The levels of mRNA were normalized to that of <i>β-actin</i>. Values are presented as the mean ± SEM, n = 3. Asterisks indicate significant differences (P < 0.05)</p

Effects of Luman knockdown via shLuman lentivirus transfection on estradiol (E2) and progesterone (P4) secretion in mouse GCs.

<p>(A~B) Concentrations of estradiol (E2) and progesterone (P4) in mouse GC culture medium after shLuman lentivirus transfection compared with shRNA-negative lentivirus transfection at 48 h. (C~O) The mRNA and protein levels of genes (<i>Star</i>, <i>Cyp19a1</i>, <i>Cyp1b1</i>, <i>Cyp11a1</i> and <i>Runx2</i>) associated with hormonal secretion were compared between the shLuman and shRNA-negative groups. The levels of mRNA were normalized to that of <i>β-actin</i>. Values are presented as the mean ± SEM, n = 3. Asterisks indicate significant differences (P < 0.05).</p

The secretion and expression of cytokines by uterine DBA⁺ leukocytes in response to embryos and steroids.

<p>ELISA analyses of the concentrations of IFN-γ (a) and VEGF (c) secreted by uterine DBA⁺ leukocytes in response to cloned and fertilized embryos. Representative western blotting and densitometric analysis of IFN-γ (b) and VEGF (d) normalized to β-actin to correct for protein loading. <i>Columns</i> and <i>vertical bars</i> represent the mean ± SEM from at least five independent experiments, and analyzed by two-way ANOVA with treatment group and/or embryos as the independent variable(s). Columns with different superscript letters are significantly different from each other (<i>P</i> < 0.05).</p

Flow cytometry analysis of the expression of CD56⁺CD16^- on goat uterine leukocytes.

<p>Representative flow cytometry analysis of the percentage of CD56⁺CD16^- cells on isolated goat uterine leukocytes in response to cloned and fertilized embryos (a). The absolute numbers of uterine CD56⁺CD16^- leukocytes over 120-hr incubation with different conditioned mediums or control medium (b). Fold expansion of total numbers of isolated goat uterine leukocytes after 120-hr incubation with different conditioned mediums or control medium in comparison to the cells at the beginning of the culture period (c). <i>Columns</i> and <i>vertical bars</i> represent the mean ± SEM from at least five independent experiments, and analyzed by two-way ANOVA with treatment group and/or embryos as the independent variable(s). Columns with different superscript letters are significantly different from each other (<i>P</i> < 0.05).</p