One-pot synthesis of α-bromoacetals of ketones from secondary alcohols and 1,3-dibromo-5,5-dimethylhydantoin (DBDMH) in ethylene glycol

<p>α-Bromoacetals of ketones were prepared from various secondary alcohols with 1,3-dibromo-5,5-dimethylhydantoin (DBDMH) and ethylene glycol through oxidation, bromination, and acetalization in one pot without the use of other catalysts under mild conditions. The effects of DBDMH, the solvent, and <i>N</i>-bromosuccinimide on the reaction were investigated. Under the optimal conditions, most α-bromoacetals of ketones were obtain in 90–98% yields.</p

Additional file 2 of Comparison of liver resection and radiofrequency ablation in long-term survival among patients with early-stage hepatocellular carcinoma: a meta-analysis of randomized trials and high-quality propensity score-matched studies

Additional file 2 Supplementary material: Supplementary material S1: Search strategy. Supplementary material S2 NOS score for PSM studies. Supplementary material S3 Risk bias of RCTs. Supplementary material S4 1-,3-,and 5-year survival rate, disease-free survival rate, and recurrence rate. Supplementary material S5 Forest plot for sensitivity analysis of overall survival and disease-free survival. Supplementary material S6 Funnel plot for overall survival and disease-free survival. Supplementary material S7 Meta-regression. OS, overall survival; DFS, disease-free survival; RFA, radiofrequency ablation. Supplementary material S8 Subgroup analysis for OS and DFS based on modality of RF

Additional file 1 of Comparison of liver resection and radiofrequency ablation in long-term survival among patients with early-stage hepatocellular carcinoma: a meta-analysis of randomized trials and high-quality propensity score-matched studies

Additional file 1 PRISMA checklis

GATA2 rs2335052 Polymorphism Predicts the Survival of Patients with Colorectal Cancer

<div><p>Background</p><p>GATA binding protein 2 (GATA2) is a transcription factor that has essential roles in hematologic malignancies and progression of various solid tumors. Our previous studies suggested that high GATA2 expression is associated with recurrence of colorectal cancer (CRC). However, the influence of GATA2 single nucleotide polymorphisms (SNPs) on the survival of CRC remains unknown.</p><p>Methods</p><p>We genotyped GATA2 SNP rs2335052 using Sanger sequencing after PCR amplification, and determined GATA2 expression by immunohistochemistry in a cohort of 180 CRC patients. Kaplan-Meier survival analysis and Cox proportional hazard regression were used to analyze the association between the GATA2 rs2335052 genotypes and the clinical outcome of CRC.</p><p>Results</p><p>We found that there was no significant correlation between the rs2335052 genotypes and the expression of GATA2. However, the Kaplan-Meier survival analysis suggested that the carriers of the A-allele of SNP rs2335052 were significantly associated with increased risk of recurrence and reduced disease-free survival (DFS), compared with those carrying the variant genotype of GG in rs2335052 (<i>P</i> = 0.021). Moreover, univariate and multivariate Cox regression analyses revealed that GATA2 SNP rs2335052 was an independent risk factor for the DFS of CRC patients.</p><p>Conclusion</p><p>Our results demonstrated that GATA2 SNP rs2335052 is an independent predictor for prognosis of CRC patients. This raised the possibility that SNP rs2335052 may serve as a potential indicator for predicting recurrence of CRC after curative colectomy.</p></div

Kaplan-Meier analysis of DFS according to GATA2 expression.

<p><b>(A)</b> Kaplan-Meier survival curve showed DFS for patients with GATA2-high tumors versus patients with GATA2-low tumors. Kaplan-Meier survival curves showed DFS for patients stratified by GATA2 rs2335052 GG <b>(B)</b>, and GG+AA <b>(C)</b> genotypes. The log-rank test was used to calculate <i>P</i> values.</p

Sequencing results of GATA2 rs2335052 genotypes.

<p>Sequencing results of GATA2 rs2335052 genotypes.</p

Kaplan-Meier analysis for DFS according to rs2335052 genotypes in CRC patients stratified by clinical stage.

<p>Kaplan-Meier curves indicated DFS for the subgroup of patients with stage I/II <b>(A)</b>, and stage III/IV <b>(B)</b> CRC. The log-rank test was used to calculate <i>P</i> values.</p

Immunohistochemical analysis of GATA2 expression in colorectal tissues.

<p>Representative image indicated strong GATA2 staining in CRC tissue, and negative GATA2 staining in matched noncancerous tissue. Magnification is 100×.</p

Univariate and multivariate analyses of GATA2 rs2335052 genotypes in CRC patients with respect to DFS.

<p><i>HR</i> hazard ratio, <i>CI</i> confidence interval, <i>P</i> values in bold were statistically significant.</p><p>Univariate and multivariate analyses of GATA2 rs2335052 genotypes in CRC patients with respect to DFS.</p

Comparative transcriptome profile of the leaf elongation zone of wild barley (Hordeum spontaneum) eibi1 mutant and its isogenic wild type

<div><p>Abstract The naturally occurring wild barley mutant eibi1/hvabcg31 suffers from severe water loss due to the permeable leaf cuticle. Eibi1/HvABCG31 encodes a full ATP-binding cassette (ABC) transporter, HvABCG31, playing a role in cutin deposition in the elongation zone of growing barley leaves. The eibi1 allele has pleiotropic effects on the appearance of leaves, plant stature, fertility, spike and grain size, and rate of germination. Comparative transcriptome profile of the leaf elongation zone of the eibi1 mutant as well as its isogenic wild type showed that various pathogenesis-related genes were up-regulated in the eibi1 mutant. The known cuticle-related genes that we analyzed did not show significant expression difference between the mutant and wild type. These results suggest that the pleiotropic effects may be a compensatory consequence of the activation of defense genes in the eibi1 mutation. Furthermore, we were able to find the mutation of the eibi1/hvabcg31 allele by comparing transcript sequences, which indicated that the RNA-Seq is useful not only for researches on general molecular mechanism but also for the identification of possible mutant genes.</p></div