Biological Interactions and Dynamics Science Theme Advisory Panel (BID-STAP)

This report contains the charge to the panel, the panel's discussions and panel recommendations

Enhancing Isoprene Production by Genetic Modification of the 1-Deoxy-d-Xylulose-5-Phosphate Pathway in Bacillus subtilis

To enhance the production of isoprene, a volatile 5-carbon hydrocarbon, in the Gram-positive spore-forming rod-shaped bacterium Bacillus subtilis , 1-deoxy- d -xylulose-5-phosphate synthase (Dxs) and 1-deoxy- d -xylulose-5-phosphate reductoisomerase (Dxr) were overexpressed in B. subtilis DSM 10. For the strain that overexpresses Dxs, the yield of isoprene was increased 40% over that by the wild-type strain. In the Dxr overexpression strain, the level of isoprene production was unchanged. Overexpression of Dxr together with Dxs showed an isoprene production level similar to that of the Dxs overproduction strain. The effects of external factors, such as stress factors including heat (48°C), salt (0.3 M NaCl), ethanol (1%), and oxidative (0.005% H 2 O 2 ) stress, on isoprene production were further examined. Heat, salt, and H 2 O 2 induced isoprene production; ethanol inhibited isoprene production. In addition, induction and repression effects are independent of SigB, which is the general stress-responsive alternative sigma factor of Gram-positive bacteria

Current Status and Prospects of Valorizing Organic Waste via Arrested Anaerobic Digestion: Production and Separation of Volatile Fatty Acids

Volatile fatty acids (VFA) are intermediary degradation products during anaerobic digestion (AD) that are subsequently converted to methanogenic substrates, such as hydrogen (H2), carbon dioxide (CO2), and acetic acid (CH3COOH). The final step of AD is the conversion of these methanogenic substrates into biogas, a mixture of methane (CH4) and CO2. In arrested AD (AAD), the methanogenic step is suppressed to inhibit VFA conversion to biogas, making VFA the main product of AAD, with CO2 and H2. VFA recovered from the AAD fermentation can be further converted to sustainable biofuels and bioproducts. Although this concept is known, commercialization of the AAD concept has been hindered by low VFA titers and productivity and lack of cost-effective separation methods for recovering VFA. This article reviews the different techniques used to rewire AD to AAD and the current state of the art of VFA production with AAD, emphasizing recent developments made for increasing the production and separation of VFA from complex organic materials. Finally, this paper discusses VFA production by AAD could play a pivotal role in producing sustainable jet fuels from agricultural biomass and wet organic waste materials