EEffect of folic acid on fluoride induced morphological alterations in the liver of albino wistar rats

Objective: To observe the histomorphological changes in liver of Albino wistar rats induced by Fluoride and effect of Folic acid.Methodology: This quasi experimental study was conducted at Anatomy department & postgraduate Laboratory of Isra University Hyderabad Sindh from April 2018 to September 2018. Total forty healthy rats 8-12 weeks old and weight 150-250gm were selected. Animals were divided in 4 groups in equal numbers. Animals of Group A, were given normal diet, Group B, received distilled water mixed with Fluoride (10.0mg/kg) and group C received distilled water mixed with Fluoride (10mg/kg) along with folic acid supplement (2.5mg/kg). Group D, animals received Fluoride (10mg/kg) for four weeks initially and then folic acid (2.5mg/kg) mixed distilled water was given for additional four weeks.  After completion of experiment, the rats were sacrificed and hepatic tissues were processed to prepare paraffin blocks. 4-6 micrometer sections were obtained for slides and stained with hematoxylin and eosin to observe under light microscope. All the data was recorded in proforma. Results: Liver weight was insignificant among all study groups. On histological examinations, fibrotic changes were significantly higher among all experimental groups as compare to control group whereas folic acid consumption reduced it. Necrotic changes, hepatic inflammatory changes, sinusoidal dilatation and congested portal veins were found higher among animals of experimental group B as compared to group C and group D, while no changes found in control group.Conclusion: Fluoride exposure that impair liver architecture, is potently supported by the portal inflammation, necrosis, and histological alterations. Folic acid is the best supplement to prevent and revert the hepatic histological alterations caused by fluorid

EEffect of folic acid on fluoride induced morphological alterations in the liver of albino wistar rats

Objective: To observe the histomorphological changes in liver of Albino wistar rats induced by Fluoride and effect of Folic acid.Methodology: This quasi experimental study was conducted at Anatomy department & postgraduate Laboratory of Isra University Hyderabad Sindh from April 2018 to September 2018. Total forty healthy rats 8-12 weeks old and weight 150-250gm were selected. Animals were divided in 4 groups in equal numbers. Animals of Group A, were given normal diet, Group B, received distilled water mixed with Fluoride (10.0mg/kg) and group C received distilled water mixed with Fluoride (10mg/kg) along with folic acid supplement (2.5mg/kg). Group D, animals received Fluoride (10mg/kg) for four weeks initially and then folic acid (2.5mg/kg) mixed distilled water was given for additional four weeks.  After completion of experiment, the rats were sacrificed and hepatic tissues were processed to prepare paraffin blocks. 4-6 micrometer sections were obtained for slides and stained with hematoxylin and eosin to observe under light microscope. All the data was recorded in proforma. Results: Liver weight was insignificant among all study groups. On histological examinations, fibrotic changes were significantly higher among all experimental groups as compare to control group whereas folic acid consumption reduced it. Necrotic changes, hepatic inflammatory changes, sinusoidal dilatation and congested portal veins were found higher among animals of experimental group B as compared to group C and group D, while no changes found in control group.Conclusion: Fluoride exposure that impair liver architecture, is potently supported by the portal inflammation, necrosis, and histological alterations. Folic acid is the best supplement to prevent and revert the hepatic histological alterations caused by fluorid

Histomorphometric Effcets of Oral use of Tobacco in Testes of Offsprings of Swiss Albino Mice

Objective: To study the gross micro structural changes in the testis of the offspring of Swiss albino mice exposed to oral use of tobacco during pregnancy. Study Design: Experimental Duration and Setting of Study: This study was conducted from July 2017 to December 2017 at Isra University Hyderabad  Methodology: ­­20 female albino mice were mated. After confirmation of pregnancy by vaginal plug they were divided into two groups; experimental A and control B of 10 each. Experimental mice of group A were given 5% tobacco in their chow diet and0water ad libitum; however, control group B were given only normal chow0diet and0water0ad libitum. After 15 days of birth the 10 male offsprings each of control and experimental mice were randomly selected. Their initial and final body weight was recorded.  They were sacrificed0by cervical dislocations and0their testes were taken away for further gross & histological0analysis.  Results: Noticeable changes were observed in the body weight and weight of testes. The mean initial weight of experimental male offspring was found to be 1.76 ± 0.33 g, while in control group it was 2.60± 0.45 g. The final weight in experimental offspring was 9.38± 0.59 g and in control group it was 12.75±0.96 g.  Statistically the difference of body weight in offspring was found to be significant (p value <0.05). The testes weight was markedly decreased in experimental group as compared to control group.  The mean testes weight in experiment group was recorded as 0.03 ± 0.004 g however in control group it was recorded as   0.07 ± 0.004 g. Simultaneously, 5 micro structural variations were also observed in the testes of off-springs of mice. In0the0experimental group0of off-springs, very few0layers and decreased number of spermatocytes were noticed in seminiferous tubules of 9 testes.  Sperm degenerative changes, cellular inflammation and mild to massive hyalinization were noticed in 9, 6, and 9 testes of experimental group respectively. Loss of architecture of seminiferous tubules in 8 testes as well as destruction of the basement membrane in 7 testes were also observed in experiment group testes. However, the decreased number of spermatocytes in only 1 testes and mild hyalinization in 2 testes of control group were observed. Conclusion: The consumption of smokeless tobacco has vital effects on the body weight, micro structure  and weight of testes of offsprings of mice given with cellular injury of seminiferous tubules especially decreased sperm count, cellular inflammation, destruction of basement membrane as well as massive hyalinization. &nbsp

Stiff Columns as Liquefaction Mitigation Measure for Retrofit of Existing Buildings

In this paper, ground reinforcement with jet grouted columns under shallow foundations of existing buildings was analysed using numerical modelling. This study is related with ground reinforcement by installing stiff jet grouted columns around the shallow foundations of existing building when the foundation soil is liquefied during an earthquake. The isolated shallow square footing pad supporting a typical simple frame structure was constructed on the reinforced ground with stiff jet grouted column rows at the shallow depth from the ground surface. This soil-structure system was modelled and analyzed as plane-strain using the FLAC (Fast Lagrangian Analysis of Continua) 2D dynamic modelling and analysis software. The results showed that liquefaction-induced large settlement of shallow foundation of existing building can be reduced to tolerable limits by applying ground reinforcement with continuous rows vertical jet grouted columns adjacent to footing pad

Effects of the Length of Jet Grouted Columns and Soil Profile on the Settlement of Shallow Foundations

In this paper, the effect of length of jet grouted columns and varying soil profile under shallow foundations of buildings constructed on the liquefiable ground was studied. The isolated shallow footing pad which supports a typical simple frame structure was constructed on the liquefiable ground. This ground was reinforced with jet grouted column rows under the shallow foundations of structure. The system was modeled as plane-strain using the FLAC 2D (Fast Lagrangian Analysis of Continua) dynamic modelling and analysis code. This case focuses on the length of jet grouted columns in a soil profile and the effect of soil profiles of varying thickness on the settlements of building structure when the soil is liquefied during an earthquake. The results show that liquefaction-induced large settlements of shallow foundation of building decrease to tolerable limits with the increase in the length of columns. For soil profiles, with a relatively thinner liquefiable layer, a certain minimum length of columns (extended in base non liquefiable layer) is required to meet the settlement tolerable limits. For soil profiles, with a relatively thicker liquefiable layer, this length should be equal to the thickness of the liquefiable layer from the footing base plus some extension in the base non liquefiable dense layer. In the soil profile with the base liquefiable layer underlying the non liquefiable layer, settlements could not be reduced to the tolerable limits even with columns of relatively larger length which may be critical

Liquefaction Potential of Silty Sand in Simple Shear

In this paper, the liquefaction potential of medium dense and dense sand layers was studied by performing constant volume (undrained) cyclic simple shear tests using cyclic simple shear apparatus. Strain-controlled approach was adopted. The liquefaction potential of two layers of a silty sand soil profile consisting of surface medium dense layer and underlying dense sand layers were studied. A medium dense sand surface layer exhibited flow type total liquefaction in cyclic loading in few cycles after initial liquefaction. An underlying dense sand base layer showed initial liquefaction in relatively more number of cycles and then cyclic mobility due to which pore pressure increases and decreases with cycles that is the characteristics of dense sand. The pore pressure increase and decrease is directly related to decrease and increase of effective stress

Estimation of Parameters and Flow Characteristics for the Design of Sanitary Sewers in Malaysia

Determination of the flow characteristics is very important for the design of sanitary sewers in any area. In the present study these are determined in the running sewers for the two parameters; per capita flow contribution and the peak flow factor. ISCO area - velocity flow meter model 4250 is used for this purpose. The flow meter, before being used in the running sewers, is calibrated first in the Hydraulics and Hydrology Laboratory of the UTM (Universiti Teknologi Malaysia). During the study the flow meter is installed inside the manhole in 10 different phases in the months of June, August, September, and October 2005 to monitor the sewage flow running in it. Continuous data is recorded in the flow meter during the process and the recorded time varies between 47 hours 25 minutes and 128 hours 35 minutes. The rainfall data is also collected during the same time using an automatic rain gauge which recorded rainfall at every five minutes of interval. Both the parameters thus calculated are then compared with the Malaysian Standard for sewer design i.e. MS 1228:1991. The results show that higher values of these parameters are being used in the design of sanitary sewers and extensive study needs to be carried out to review these values for future us

Effect of Variation in Blending Variables on the Properties of CRMB

The enhancement in the properties of the modified blends largely depends on the proper understanding of the interaction between CR (Crumb Rubber) and bitumen, where the CR particles swell after absorbing lighter fraction from the bitumen phase. However, the properties of CRMB (Crumb Rubber Modified Bitumen) blends at a wide range of temperatures are considered to be somewhat unclear due to the various interaction effects of CR and base bitumen. This largely depends on the interaction conditions such as blending time, temperature, speed and device, which may alter the properties of the blend to great extent. In this study, influence of the interaction condition was investigated by looking at the viscosity with a Brookfield viscometer. For this, CR and bitumen proportions along with other material characteristics were kept constant to understand the effect of interaction parameters of the properties of the CRMB blend. A total of 12 CRMB blends were produced with unvarying combinations of material constituents. However, selected variation in the blending parameters were; blending device, duration, speed and temperature. Observations from this laboratory study indicated: (1) blending performed with the high shear mixer resulted in higher viscosity values compared to the blends produced with the low shear mixer; (2) reduced blending time was required to achieve peak and ultimate stable viscosities, when blends were produced with the high shear mixer; (3) blending temperature of 180°C has resulted in the blends with consistent properties for longer blending durations compared to the blends produced at higher temperature. As a result, results with high shear mixer were always promising, which required comparatively lower interaction temperature, time and speed

A Conditioned Model for Choice of Mode Under Information

This paper examines the influence of time and cost information obtained from different sources on choice of mode of Leeds' long distance travellers. The choice of mode was investigated through modal attributes provided by at least two different information sources which might provide contrary or corroborating information rather than on actual attributes. The experiment included telephone administered questionnaire including RP (Revealed Preference) questions and an SP (Stated Preference) exercise dealing with the choice of modes conditioned by the information received from various sources. Information on travel time and cost was provided from two different information sources for each mode to facilitate the conditioning of mode choice on corroborating/contradictory information. The research employs a wide range of modelling methodologies and examines a range of traditional and newly developed calibration and estimation procedures including Mixed Logit models with individual specific parameters and the newly developed RRM (Random Regret Minimisation) framework. The study confirms that the market share of the modes increases when information sources show decreased travel time and cost values and shows that the maximum shares are achieved when different information sources give the same information to the travellers. The study found that pre-trip time information has more influence on mode choice when derived from websites than when derived from other sources. Pre-trip information on costs was, however, less influential when derived from websites than when derived from other sources

Influence of the Scrap Tyre Processing Techniques on the Physical Properties of the Crumb Rubber

The processing mechanism of scrap tyres to produce CR (Crumb Rubber) has a great influence on the properties of the CRMB (Crumb Rubber Modified Bitumen). A fair amount of research was observed in the literature on the bitumen modified by CR processed cryogenically and ambiently. However, little or no work has been done on the CR processed by the other methods such as the Aquablast processing technique, which is a relatively a new waste tyre rubber processing technique within the CR industry. In this study, CR obtained from the two sources; ambient and Aquablast grinding techniques were primarily assessed with a helium pycnometer and SEM (Scanning Electron Microscopy) for their physical properties and appearance respectively. Observations from this laboratory study indicated: (1) the density of the ambient CR was slightly higher than the CR obtained from Aquablast technique; (2) difference in physical appearance was found insignificant