Analysis of Structural Changes with High Resolution Structures for Lossless Ion Manipulations (SLIM) Ion Mobility-Mass Spectrometry (ASMS 2016)

<p>The availability of commercial ion mobility spectrometry (IMS) instrumentation coupled to mass spectrometry (i.e. IM-MS) has resulted in an explosion of applications. For example, IM-MS has been applied to bottom up proteomics, intact protein structural analyses (e.g. including glycoforms and quaternary structure), lipidomics, metabolomics, small molecule binding interactions and kinetics, etc. However, the ability to analyze small structural changes to proteins due to e.g. small molecule binding or post-translational modifications is limited on current platforms due to insufficient ion mobility resolution in many cases for separation of key spectrum components. We will present an investigation into protein structural changes by the application of a high IMS resolution SLIM Structures for Lossless Ion Manipulations module, utilizing a multi-pass arrangement.</p