Analyzing Energy-efficiency and Route-selection of Multi-level Hierarchal Routing Protocols in WSNs

The advent and development in the field of Wireless Sensor Networks (WSNs) in recent years has seen the growth of extremely small and low-cost sensors that possess sensing, signal processing and wireless communication capabilities. These sensors can be expended at a much lower cost and are capable of detecting conditions such as temperature, sound, security or any other system. A good protocol design should be able to scale well both in energy heterogeneous and homogeneous environment, meet the demands of different application scenarios and guarantee reliability. On this basis, we have compared six different protocols of different scenarios which are presenting their own schemes of energy minimizing, clustering and route selection in order to have more effective communication. This research is motivated to have an insight that which of the under consideration protocols suit well in which application and can be a guide-line for the design of a more robust and efficient protocol. MATLAB simulations are performed to analyze and compare the performance of LEACH, multi-level hierarchal LEACH and multihop LEACH.Comment: NGWMN with 7th IEEE Inter- national Conference on Broadband and Wireless Computing, Communication and Applications (BWCCA 2012), Victoria, Canada, 201

Isolation of Acanthamoeba isolates belonging to T2, T3, T4 and T7 genotypes from environmental samples in Ankara, Turkey

Acanthamoeba keratitis is a blinding infection that is becoming increasingly important in human health. Early diagnosis is a prerequisite for successful treatment and requires identification of Acanthamoeba at the genotypic level. The genus Acanthamoeba consists of both pathogenic and non-pathogenic species and has been recently classified into 13 different genotypes, T1-T12 and T14. More importantly, 95% of Acanthamoeba isolates that produce keratitis belong to T4 genotypes. In this study, we attempted to determine whether predominance of T4 isolates in Acanthamoeba keratitis is due to greater virulence or greater prevalence. We isolated 18 Acanthamoeba isolates from environmental samples in Ankara, Turkey and determined their pathogenic potential by means osmotolerance, temperature tolerance and in vitro cytotoxicity assays using corneal epithelial cells. Ribosomal DNA sequencing revealed that 10 isolates belong to T2, 5 belong to T3, 2 belong to T4 and one belongs to T7 genotype. As expected, T3 and T4 isolates exhibited the most pathogenic traits and were osmotolerant, temperature tolerant and exhibited severe corneal epithelial cell cytotoxicity indicating their pathogenic potential. Overall these data indicate that high frequency of T4 isolates in keratitis cases may well be due to their greater virulence. This is the first report presenting environmental distribution of Acanthamoeba in Ankara, Turkey

Acanthamoeba castellanii induces host cell death via a phosphatidylinositol 3-kinase-dependent mechanism

Granulomatous amoebic encephalitis due to Acanthamoeba castellanii is a serious human infection with fatal consequences, but it is not clear how the circulating amoebae interact with the blood-brain barrier and transmigrate into the central nervous system. We studied the effects of an Acanthamoeba encephalitis isolate belonging to the T1 genotype on human brain microvascular endothelial cells, which constitute the blood-brain barrier. Using an apoptosis-specific enzyme-linked immunosorbent assay, we showed that Acanthamoeba induces programmed cell death in brain microvascular endothelial cells. Next, we observed that Acanthamoeba specifically activates phosphatidylinositol 3-kinase. Acanthamoeba-mediated brain endothelial cell death was abolished using LY294002, a phosphatidylinositol 3-kinase inhibitor. These results were further confirmed using brain microvascular endothelial cells expressing dominant negative forms of phosphatidylinositol 3-kinase. This is the first demonstration that Acanthamoeba-mediated brain microvascular endothelial cell death is dependent on phosphatidylinositol 3-kinase

Mechanics insights of curcumin in myocardial ischemia: Where are we standing?

Cardiovascular disorders are known as one of the main health problems which are associated with mortality worldwide. Myocardial ischemia (MI) is improper blood supply to myocardium which leads from serious complications to life-threatening problems like AMI, atherosclerosis, hypertension, cardiac-hypertrophy as well as diabetic associated complications as diabetic atherosclerosis/cardiomyopathy/hypertension. Despite several efforts, the current therapeutic platforms are not related with significant results. Hence, it seems, developing novel therapies are required. In this regard, increasing evidences indicated, curcumin (CRC) acts as cardioprotective agent. Given that CRC and its analogs exert their cardioprotective effects via affecting on a variety of cardiovascular diseases-related mechanisms (i.e., Inflammation, and oxidative stress). Herein, for first time, we have highlighted the protective impacts of CRC against MI. This review might be a steppingstone for further investigation into the clinical implications of the CRC against MI. Furthermore, it pulls in light of a legitimate concern for scientific community, seeking novel techniques and characteristic dynamic biopharmaceuticals for use against myocardial ischemia. © 2019 Elsevier Masson SA

Confirmation of sunflower F1 hybrids using SDS-PAGE analysis

Among bio-chemical techniques, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDSPAGE) is most widely used due to its validity and simplicity for describing genetic structures of group of plants. In the present study, SDS-PAGE was used to confirm sunflower hybrids. Four male sterile lines, four restorer lines and their sixteen F1 hybrids were analyzed. Based on results of electrophoretic band spectra, Jaccard’s similarity index (S) was calculated for all possible pairs. The similarity matrix, thus, generated was converted to a dissimilarity matrix and used to construct dendrogram, using unweighed pair-group method with arithmetic means. Euclidean distance of 1.0 was observed among 5 comparisons, whereas maximum distance (3.87) was observed for 1 comparison only. In most of the cases, female parents, along with their respective crosses, were found in one cluster, indicating thatSDS-PAGE can be used reliably for the identification of hybrids in sunflower

Influence of pH, temperature and glucose on biodegradation of 4-aminophenol by a novel bacterial strain, Pseudomonas sp. ST-4

Aromatic compounds such as 4-aminophenol are toxic to the environment and thus should be eliminated effectively. Biodegradation of aromatic compounds is an efficient and environment friendly technique as addition of selected microbes does not add any kind of pollutants and actively remove even the most recalcitrant pollutants. We investigated the effectiveness of Pseudomonas sp. strain ST- 4 in the biodegradation of 4-aminophenol under variable pH, temperature and glucose regimes and sorted out optimum conditions for maximum biodegradation of 4-aminophenol. Maximum biodegradation of 4-aminophenol by Pseudomonas sp. strain ST- 4 was observed at pH 8, temperature 30°C and glucose concentration of 15 mM at 72 h, respectively