Direct Extraction and Assessment of Genomic DNA of Mycetoma Fungi from Black-grains Specimen

Background: Direct isolation of genomic DNA of mycetoma fungi from black-grains achieve rapid diagnosis and may overcome culture disadvantages. Objectives: This study aimed to isolate and assess the DNA of mycetoma fungi using black-grains and to apply amplification of ITS region and nucleotide sequences. Methods: CTAB method was followed by manual homogenization alternatively to liquid nitrogen and glass beads disruption to obtain the genomic DNA. Results: Yielded DNA concentrations vary from 1.50 to 47.97 μg/ml (mean 10.09 μg/ml) while the optimum DNA purity recorded with 75.8% of specimens (n=69/91).Successful amplification of ITS region was done using pan-fungal primers (ITS4/5) with 90.1 (n=82/91)percentage. Species nucleotide sequences were detected with 67 (94.4%) amplicons from a total of 71.Conclusion: The study recommended using of black-grain specimens for DNA extraction of mycetoma fungi parallel with culture to insure rabid diagnosis and identification

Expression of E-cadherin, N-cadherin, and Cytokeratin 18 and 19 in Placentas of Women with Severe Preeclampsia

BACKGROUND: Although the exact mechanism leading to preeclampsia is not fully understood, abnormal trophoblast invasion contributes to its pathogenesis. Keratins and cadherin are known to play roles in the regulation of trophoblast proliferation. However, studies describing the association between keratins, cadherin, and preeclampsia are limited. AIM: The current study was conducted to investigate the association of these proteins with severe preeclampsia in Sudanese women. METHODS: A case–control study was conducted at Madani Maternity Hospital, Sudan. The cases included women with severe preeclampsia (n = 56) and healthy pregnant women as controls (n = 56). The assessment of keratin and cadherin was performed using immunohistochemical staining. RESULTS: There was no significant difference between the two groups in their mean age or parity. We found no significant differences in the expression of the markers E-cadherin, N-cadherin, or cytokeratin 18 and 19 in the placentas from individuals with preeclampsia versus controls. The number of placentas with severe preeclampsia versus controls expressing the E-cadherin, N-cadherin, cytokeratin 18, and cytokeratin 19 markers was 46 (82.1%) versus 46 (82.1%) (p = 0.988), 54 (96.4%) versus 48 (85.7%) (p = 0.121), 4 (7.1%) versus 0 (0%) (p = 0.126), and 11 (19.6%) versus 11 (19.6%) (p = 0.532), respectively. There was also no significant difference in the intensity of staining of these four markers (Ecadherin, N-cadherin, and cytokeratin 18 and 19) between severe preeclampsia and control placentas. CONCLUSION: Together, these results indicate that in this setting, the expression of E-cadherin, N-cadherin, CK18, and CK19 is not associated with severe preeclampsia

Do grain reserves necessarily contribute to prices stability and food security in Sudan? An assessment

AbstractMost governments in Africa implement policies aiming to stabilize the prices of staple foods, which often include building up grain reserves, besides other trade measures insulating their domestic market from the world market. The mechanism should ideally work as follows, grains should be bought and stored from areas, during the surplus seasons (after harvest) so as to assure fair prices to producers and should be distributed during deficit seasons, in deficit areas besides in cases of emergencies. However, ideal approaches are not necessarily followed in many developing countries due to different constraints and situations. The Strategic Reserve Corporation (SRC) is an institution that is established ten years ago to play such a role in Sudan. This paper tries to assess the performance of the SRC against the overall goals and to study the related obstacles if any. We use a sample of 112 respondents from the SRC staff, related and grain farmers as our data source. Results of the research revealed numerous financial and administrative constraints that obstruct SRC from playing the intended role, which need to be considered so as to contribute to price stability and food security in Sudan

Pattern of global spin alignment of ϕ and K*0 mesons in heavy-ion collisions

Notwithstanding decades of progress since Yukawa first developed a description of the force between nucleons in terms of meson exchange1, a full understanding of the strong interaction remains a considerable challenge in modern science. One remaining difficulty arises from the non-perturbative nature of the strong force, which leads to the phenomenon of quark confinement at distances on the order of the size of the proton. Here we show that, in relativistic heavy-ion collisions, in which quarks and gluons are set free over an extended volume, two species of produced vector (spin-1) mesons, namely ϕ and K*0, emerge with a surprising pattern of global spin alignment. In particular, the global spin alignment for ϕ is unexpectedly large, whereas that for K*0 is consistent with zero. The observed spin-alignment pattern and magnitude for ϕ cannot be explained by conventional mechanisms, whereas a model with a connection to strong force fields2,3,4,5,6, that is, an effective proxy description within the standard model and quantum chromodynamics, accommodates the current data. This connection, if fully established, will open a potential new avenue for studying the behaviour of strong force fields

Evaluation of Serum Copper, Zinc and Copper/Zinc Ratio in Psoriatic Patients

Backgrounds: Psoriasis is a hyper proliferative coetaneous disease of multi-factorial etiologies, genetic background, environmental factors, vascular and immune system disturbances. Copper and zinc are very importance trace elements and essential to biochemical processes in the body and are involved in immunological and inflammatory reactions. Objective: The objective of the present study was to evaluate the relationship between zinc (Zn) and copper (Cu) ion and copper/zinc with the pathogenesis of psoriasis disease. Materials and Methods: an analytical case control hospital based study conducted in Khartoum dermatology and venerology teaching hospital, Khartoum state, Sudan. During the period from September to December 2018. A total of 100 subjects were enrolled in the study, 50 psoriatic patients as case group and 50 apparently healthy as control group with match age and sex. The age ranged from 12 and 60 years and their average mean 32 years. Three ml of venous blood were collected from each volunteer, serum was obtained and analyzed using colorimetric method in fully Automated Biochemistry Analyzer and the data was analyzed using SPSS version (21). P-value of <0.05 was considered significant. And also psoriatic patients the severity of psoriasis was assessed by ‘Psoriasis Area Severity Index’ (PASI score). Results: The study revealed a significantly increase (p = 0.000) of serum copper in psoriatic patients compared to control group and a significantly decrease (p = 0.040) of serum zinc in psoriatic patients compared to control group. And the copper/ zinc was significantly increase (p = 0.000) in patients than controls. Moreover, serum copper was  significantly increased (p = 0.012) in psoriatic patients infected >1 year compared to patients infected 6 months to 1 year. The serum zinc was significantly decreased (p = 0.030) in psoriatic patients infected >1 year compared to patients infected 6 month-1 year. And also the copper/ zinc significantly increased (p = 0.027) in psoriatic patients infected >1 year compared to patients infected 6 month to 1 year. The serum copper was a significantly different (p =0.001) between severity of psoriatic disease compared to mild and moderate. Moreover, the serum zinc was  significantly different (p =0.001) between mild and moderate and also a significantly different (p =0.022) between severity of psoriatic disease compared to mild and moderate. The copper/ zinc was a significantly different (p =0.000) between severity of psoriatic disease compared to mild and moderate. Conclusion: The psoriatic patients had a significantly increased serum copper, copper/ zinc and a significantly decrease of serum zinc. The study observed that, the common in male than female and also in moderate a stage of severity

Meta-analysis of honey bee neurogenomic response links deformed wing virus type A to precocious behavioral maturation

Crop pollination by the western honey bee Apis mellifera is vital to agriculture but threatened by alarmingly high levels of colony mortality, especially in Europe and North America. Colony loss is due, in part, to the high viral loads of Deformed wing virus (DWV), transmitted by the ectoparasitic mite Varroa destructor, especially throughout the overwintering period of a honey bee colony. Covert DWV infection is commonplace and has been causally linked to precocious foraging, which itself has been linked to colony loss. Taking advantage of four brain transcriptome studies that unexpectedly revealed evidence of covert DWV-A infection, we set out to explore whether this effect is due to DWV-A mimicking naturally occurring changes in brain gene expression that are associated with behavioral maturation. Consistent with this hypothesis, we found that brain gene expression profiles of DWV-A infected bees resembled those of foragers, even in individuals that were much younger than typical foragers. In addition, brain transcriptional regulatory network analysis revealed a positive association between DWV-A infection and transcription factors previously associated with honey bee foraging behavior. Surprisingly, single-cell RNA-Sequencing implicated glia, not neurons, in this effect; there are relatively few glial cells in the insect brain and they are rarely associated with behavioral plasticity. Covert DWV-A infection also has been linked to impaired learning, which together with precocious foraging can lead to increased occurrence of infected bees from one colony mistakenly entering another colony, especially under crowded modern apiary conditions. These findings provide new insights into the mechanisms by which DWV-A affects honey bee health and colony survival

Evolution of Patient Dose in Chest Radiotherapy Planning

Radiographic image has been used for patient positioning, target localization radiation beam alignment, and subsequent verification of treatment delivery in radiotherapy. Radiographic imaging as all medical use of ionizing radiation can give significant exposure to the patient. The aim of this study was to determine the radiological dose for chest imaging. Imaging dose during course of radiotherapy add dose to high therapeutic dose therefore this raises the issue of the balance between the benefit of these additional imaging exposures and the associated risk of radiation induced cancer arising from them. Therefore, estimation of imaging doses and possibility of its risk is necessary to provide adequate justification of this exposure. In this dissertation the main investigated type of the X-ray simulation were chest AP and PA, the total number of patients was 10 ( 62 radiographs). The fluctuation of the entrance surface dose (ESD) was relatively ranging from 0.35 micro;Gy to 8.43 micro;Gy for AP projection, and from 0.12 micro;Gy to 0.46 micro;Gy for PA projection. The mean values of ESD were found to be within guidance limits which was proposed in some countries (CEC 2004, and Germany 2003)

Mitosis Detection, Fast and Slow: Robust and Efficient Detection of Mitotic Figures

Counting of mitotic figures is a fundamental step in grading and prognostication of several cancers. However, manual mitosis counting is tedious and time-consuming. In addition, variation in the appearance of mitotic figures causes a high degree of discordance among pathologists. With advances in deep learning models, several automatic mitosis detection algorithms have been proposed but they are sensitive to {\em domain shift} often seen in histology images. We propose a robust and efficient two-stage mitosis detection framework, which comprises mitosis candidate segmentation ({\em Detecting Fast}) and candidate refinement ({\em Detecting Slow}) stages. The proposed candidate segmentation model, termed \textit{EUNet}, is fast and accurate due to its architectural design. EUNet can precisely segment candidates at a lower resolution to considerably speed up candidate detection. Candidates are then refined using a deeper classifier network, EfficientNet-B7, in the second stage. We make sure both stages are robust against domain shift by incorporating domain generalization methods. We demonstrate state-of-the-art performance and generalizability of the proposed model on the three largest publicly available mitosis datasets, winning the two mitosis domain generalization challenge contests (MIDOG21 and MIDOG22). Finally, we showcase the utility of the proposed algorithm by processing the TCGA breast cancer cohort (1,125 whole-slide images) to generate and release a repository of more than 620K mitotic figures.Comment: Extended version of the work done for MIDOG challenge submissio