Potential bioactivities of -mangostin from garcinia malaccensis Hk.f

Guttiferae family is well-known to have a wide range of phytochemical constituents and bioactivities. A phytochemical investigation of Garcinia malaccensis lead isolation of -mangostin, -mangostin and a triterpenoid. α-Mangostin, a xanthone has a lot of health benefits. Many studies have been reported to investigate the biological activities of α-mangostin. The present study was carried out to evaluate the antimicrobial, antioxidant and anticancer activities of α-mangostin. Its structural determination was done based on its spectroscopic analysis. α-Mangostin was tested for antimicrobial sensitivity via disc diffusion method against 4 bacteria. Results showed that S. aureus culture formed a clear inhibition zone. The diameter of zone of inhibition observed was 8 mm and minimum inhibition concentration (MIC) value was 0.025 mg/mL and minimum bactericidal concentration (MBC) value was 0.1 mg/mL, indicated that α-mangostin is a bacteriostatic and bactericidal agent which correlates to presence of hydroxyl group in its structure. In antioxidant properties tests, dot-blot DPPH staining showed a positive antioxidant activity of α-mangostin. In FTC method, α-mangostin was proved to be a good lipid peroxidation inhibitor, whereas in DPPH free radical scavenging activity method, it has very weak scavenging effects on free radicals. In antiproliferative assay, -mangostin exhibited activity against K562 and showed different activity against HSC3 and H1299 cell lines. Against K562, it exerted the value of IC50 20 µg/mL. This study can form a foundation for future studies in investigating of biological activities of α-mangostin and developing the natural abundant in improving a healthy community

Mangostins and their bioactivities from garcinia Malaccensis HK.F

Introduction: Guttiferae is a well-known family having a wide range of phytochemical constituents and bioactivities. Objectives: To investigate phytochemicals and bioactivities of Garcinia malaccensis. Materials and Methods: The isolated compounds were elucidated by spectroscopic methods. Antimicrobial activity was carried out by disc diffusion method. Antiproliferative activity was performed by tryphan blue exclusion and MTT assay. Antioxidant activity was conducted by DPPH radical scavenging. Results: Phytochemicals investigation of the stem bark of G. malaccensis lead to isolation of -mangostin, -mangostin and a triterpenoid. The main priority of this study’s activity was done on -mangostin as the compound was found as a major constituent. Results showed that -mangostin inhibits Staphylococcus aureus. The diameter of inhibition zone observed was 8 mm, minimum inhibition concentration (MIC) was 0.025 mg/mL and minimum bactericidal concentration (MBC) was 0.1 mg/mL. Dot-blot DPPH staining showed a positive antioxidant activity of -mangostin. In FTC method, -mangostin was proved to be a good lipid peroxidation inhibitor, whereas in DPPH free radical scavenging activity method, it has a very weak scavenging effects on free radicals. In the antiproliferative assay, -mangostin exhibited activity against K562 (IC50 20 μg/mL) and showed a weak activity against HSC3 and H1299 cell lines

Chemical constituents and bioactivities of Garcinia malaccensis Hk.f

Garcinia malaccensis Hk.f (Guttiferae) is closely resembled to G. mangostana and G. hombroniana. The genus of Garcinia, especially Garcinia mangostana is well known as a medicinal plant in South-east Asia and is used in traditional medicine. The paper reported the chemical constituents of the stembark of Garcinia malaccensis together with cytotoxic, antimicrobial and antioxidant activities. Purification and structure elucidation were carried out by chromatographic and spectroscopic techniques, respectively. MTT and trypan blue exclusion methods were performed to study the cytotoxic activity. Antibacterial activity was conducted by disc diffusion and microdilution methods, whereas antioxidant activities were done by ferric thiocyanate method and DPPH radical scavenging. The phytochemical study led to the isolation of -mangostin and cycloart-24-en-3-ol. -Mangostin exhibited cytotoxic activity against HSC-3 cells with an IC50 of 0.33 M. β- and α-mangostin showed activity against K562 cells with IC50 of 0.40 M and 0.48 M, respectively. -Mangostin was active against Gram-positive bacteria, Staphylococcus aureus and Bacilus anthracis with inhibition zone and MIC value of (19 mm; 0.025 mg/mL) and (20 mm; 0.013 mg/mL), respectively. In antioxidant assay, -mangostin exhibited activity as an inhibitor of lipid peroxidation. G. malaccensis presence - and -mangostin and cycloart-24-en-3-ol. -Mangostin was found very active against HSC-3 cells and K562. The results suggest that mangostins derivatives have the potential to inhibit the growth of cancer cells by inducing apoptosis. In addition, -and -mangostin was found inhibit the growth of Gram-positive pathogenic bacteria and also showed the activity as an inhibitor of lipid peroxidation. Keywords: Garcinia malaccensis, Guttiferae, apoptosis, antibacterial, antioxidan

Apoptosis, antimicrobial and antioxidant activities of Phytochemicals from Garcinia malaccensis HK.F

Objective: To study the chemical constituents of stembark of Garcinia malaccensis (G. malaccensis) together with apoptotic, antimicrobial and antioxidant activities. Methods: Purification and structure elucidation were carried out by chromatographic and spectroscopic techniques, respectively. MTT and trypan blue exclusion methods were performed to study the cytotoxic activity. Antibacterial activity was conducted by disc diffusion and microdilution methods, whereas antioxidant activities were done by ferric thiocyanate method and DPPH radical scavenging. Results: The phytochemical study led to the isolation of α,β-mangostin and cycloart-24-en-3β-ol. α-Mangostin exhibited cytotoxic activity against HSC-3 cells with an IC 50 of 0.33 μM. β- and α-mangostin showed activity against K562 cells with IC 50 of 0.40 μM and 0.48 μM, respectively. α-Mangostin was active against Gram-positive bacteria, Staphylococcus aureus (S. aureus) and Bacilus anthracis (B. anthracis) with inhibition zone and MIC value of (19 mm; 0.025 mg/mL) and (20 mm; 0.013 mg/mL), respectively. In antioxidant assay, α-mangostin exhibited activity as an inhibitor of lipid peroxidation. Conclusions: G. malaccensis presence α- and β-mangostin and cycloart-24-en-3β-ol. β-Mangostin was found very active against HSC-3 cells and K562. The results suggest that mangostins derivatives have the potential to inhibit the growth of cancer cells by inducing apoptosis. In addition, α-and β-mangostin was found inhibit the growth of Gram-positive pathogenic bacteria and also showed the activity as an inhibitor of lipid peroxidation

Apoptosis, antimicrobial and antioxidant activities of phytochemicals from Garcinia malaccensis Hk.f

Objective: The paper reported chemical constituents of stembark of Garcinia malaccensis together with apoptotic, antimicrobial and antioxidant activities. Methods: Purification and structure elucidation were carried out by chromatographic and spectroscopic techniques, respectively. MTT and trypan blue exclusion methods were performed to study the cytotoxic activity. Antibacterial activity was conducted by disc diffusion and microdilution methods, whereas antioxidant activities were done by ferric thiocyanate method and DPPH radical scavenging. Results: The phytochemical study led to the isolation of -mangostin and cycloart-24-en-3-ol. -Mangostin exhibited cytotoxic activity against HSC-3 cells with an IC50 of 0.33 M. β- and α-mangostin showed activity against K562 cells with IC50 of 0.40 M and 0.48 M, respectively. -Mangostin was active against Gram-positive bacteria, Staphylococcus aureus and Bacilus anthracis with inhibition zone and MIC value of (19 mm; 0.025 mg/mL) and (20 mm; 0.013 mg/mL), respectively. In antioxidant assay, -mangostin exhibited activity as an inhibitor of lipid peroxidation. Conclusion: G. malaccensis presence - and -mangostin and cycloart-24-en-3-ol. -Mangostin was found very active against HSC-3 cells and K562. The results suggest that mangostins derivatives have the potential to inhibit the growth of cancer cells by inducing apoptosis. In addition, -and -mangostin was found inhibit the growth of Gram-positive pathogenic bacteria and also showed the activity as an inhibitor of lipid peroxidation