Molecular weight of separated ES products polypeptides of <i>Fasciola gigantica</i> by SDS-PAGE.

<p>Molecular weight of separated ES products polypeptides of <i>Fasciola gigantica</i> by SDS-PAGE.</p

Immunolocalization and immunodetection of the excretory/secretory (ES) antigens of <i>Fasciola gigantica</i> - Fig 4

<p>Confocal micrograph of <i>Fasciola gigantica</i> in the liver of infected rabbit showing quite restricted immunolocalization of excretory secretory antigens as revealed by using FITC conjugated secondary antibody (A), counter staining using phalloidin TRITC (B) was done for actin localization. (C) Showing the merged A and B images, where green fluorescence is representing exclusive ES antigen distribution. [V vitelline gland, Tg tegument, Ht host tissue, Vs ventral sucker and HPi host-parasite interface].</p

Western blot analysis of ES antigens of <i>Fasciola gigantica</i> using anti-FgES antibody.

<p>Western blot analysis of ES antigens of <i>Fasciola gigantica</i> using anti-FgES antibody.</p

SDS-polyacrylamide gel electrophoresis of excretory secretory products collected from <i>Fasciola gigantica</i> after 1h, 2h and 3h of incubation in Hanks’ medium (HBSS) Std: Standard molecular weight marker.

<p>SDS-polyacrylamide gel electrophoresis of excretory secretory products collected from <i>Fasciola gigantica</i> after 1h, 2h and 3h of incubation in Hanks’ medium (HBSS) Std: Standard molecular weight marker.</p

Differential immunolocalization of ES antigens in Fasciola gigantica as revealed by confocal microscopy, using anti-Fg ES hyperimmune sera and the FITC conjugated secondary antibody.

<p>Differential immunolocalization of ES antigens in Fasciola gigantica as revealed by confocal microscopy, using anti-Fg ES hyperimmune sera and the FITC conjugated secondary antibody.</p

Dot blot analysis of faecal samples collected from field, showing three positive <i>Fasciola gigantica</i> infected samples (within the red circle) detected by polyclonal antibodies raised against <i>Fasciola gigantica</i> ES products.

<p>Dot blot analysis of faecal samples collected from field, showing three positive <i>Fasciola gigantica</i> infected samples (within the red circle) detected by polyclonal antibodies raised against <i>Fasciola gigantica</i> ES products.</p