4 research outputs found
Enhanced fluorescence of a dye on DNA-assembled gold nanodimers discriminated by lifetime correlation spectroscopy
The surface plasmon modes of metal nanoparticles provide a way to efficiently enhance the excitation and emission from a fluorescent dye. We have employed DNA-directed assembly to prepare dimers of gold nanoparticles and used their longitudinally coupled plasmon mode to enhance the fluorescence emission of an organic red-emitting dye, Atto-655. The plasmon-enhanced fluorescence of this dye using dimers of 80 nm particles was measured at the single-molecule detection level. The top enhancement factors were above 1000-fold in 71% of the dimers within a total of 32 dimers measured, and in some cases, they reached almost 4000-fold, in good agreement with model simulations. Additionally, fluorescence lifetime correlation analysis enabled the separation of enhanced from nonenhanced emission simultaneously collected in our confocal detection volume. This approach allowed us to recover a short relaxation component exclusive to enhanced emission that is attributed to the interaction of the dye with DNA in the interparticle gaps. Indeed, the frequency of enhancement events is larger than that expected from the volume occupancy of the gap region, thus suggesting that the interaction of the dye with DNA linkers favors the observation of emission enhancement in our dimer particles
Enhanced-Fluorescence of a Dye on DNA- assembled Gold Nano-Dimers Discriminated by Lifetime Correlation Spectroscopy
We have employed DNA-directed assembly to
prepare dimers of gold nanoparticles and used their longitudinally coupled plasmon mode to
enhance the fluorescence emission of an organic red-emitting dye, Atto-655. The plasmon-
enhanced fluorescence of this dye using dimers of 80 nm particles was measured at single
molecule detection level. The top enhancement factors were above 1000-fold in 71% of the
dimers within a total of 32 dimers measured, and, in some cases, they reached almost 4000-fold,
in good agreement with model simulations. Additionally, fluorescence lifetime correlation
analysis enabled the separation of enhanced from non-enhanced emission simultaneously
collected in our confocal detection volume. This approach allowed us to recover a short
relaxation component exclusive to enhanced emission that is attributed to the interaction of the
dye with DNA in the interparticle gaps.
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Enhanced fluorescence of a dye on DNA-assembled gold nanodimers discriminated by lifetime correlation spectroscopy
\u3cp\u3eThe surface plasmon modes of metal nanoparticles provide a way to efficiently enhance the excitation and emission from a fluorescent dye. We have employed DNA-directed assembly to prepare dimers of gold nanoparticles and used their longitudinally coupled plasmon mode to enhance the fluorescence emission of an organic red-emitting dye, Atto-655. The plasmon-enhanced fluorescence of this dye using dimers of 80 nm particles was measured at the single-molecule detection level. The top enhancement factors were above 1000-fold in 71% of the dimers within a total of 32 dimers measured, and in some cases, they reached almost 4000-fold, in good agreement with model simulations. Additionally, fluorescence lifetime correlation analysis enabled the separation of enhanced from nonenhanced emission simultaneously collected in our confocal detection volume. This approach allowed us to recover a short relaxation component exclusive to enhanced emission that is attributed to the interaction of the dye with DNA in the interparticle gaps. Indeed, the frequency of enhancement events is larger than that expected from the volume occupancy of the gap region, thus suggesting that the interaction of the dye with DNA linkers favors the observation of emission enhancement in our dimer particles.\u3c/p\u3
Enhanced-Fluorescence of a Dye on DNA- assembled Gold Nano-Dimers Discriminated by Lifetime Correlation Spectroscopy
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<p>We have employed DNA-directed assembly to
prepare dimers of gold nanoparticles and used their longitudinally coupled plasmon mode to
enhance the fluorescence emission of an organic red-emitting dye, Atto-655. The plasmon-
enhanced fluorescence of this dye using dimers of 80 nm particles was measured at single
molecule detection level. The top enhancement factors were above 1000-fold in 71% of the
dimers within a total of 32 dimers measured, and, in some cases, they reached almost 4000-fold,
in good agreement with model simulations. Additionally, fluorescence lifetime correlation
analysis enabled the separation of enhanced from non-enhanced emission simultaneously
collected in our confocal detection volume. This approach allowed us to recover a short
relaxation component exclusive to enhanced emission that is attributed to the interaction of the
dye with DNA in the interparticle gaps. </p>
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