Exact static solutions in four dimensional Einstein-Maxwell-Dilaton gravity

Classes of exact static solutions in four-dimensional Einstein-Maxwell-Dilaton gravity are found. Besides of the well-known solutions previously found in the literature, new solutions are presented.It's shown that spherically symmetric solutions, except the case of charged dilaton black hole, represent globally naked strong curvature singularities.Comment: 8 pages, late

Geometrical features of (4+d) gravity

We obtain the vacuum spherical symmetric solutions for the gravitational sector of a (4+d)-dimensional Kaluza-Klein theory. In the various regions of parameter space, the solutions can describe either naked singularities or black-holes or wormholes. We also derive, by performing a conformal rescaling, the corresponding picture in the four-dimensional space-time.Comment: 10 pages, LateX2e, to appear in Phys.Rev.

Electropermeabilization of inner and outer cell membranes with microsecond pulsed electric field. Quantitative study with calcium ions

Microsecond pulsed electric fields (mu sPEF) permeabilize the plasma membrane (PM) and are widely used in research, medicine and biotechnology. For internal membranes permeabilization, nanosecond pulsed electric fields (nsPEF) are applied but this technology is complex to use. Here we report that the endoplasmic reticulum (ER) membrane can also be electropermeabilized by one 100 mu s pulse without affecting the cell viability. Indeed, using Ca2+ as a permeabilization marker, we observed cytosolic Ca2+ peaks in two different cell types after one 100 mu s pulse in a medium without Ca2+. Thapsigargin abolished these Ca2+ peaks demonstrating that the calcium is released from the ER. Moreover, IP3R and RyR inhibitors did not modify these peaks showing that they are due to the electropermeabilization of the ER membrane and not to ER Ca2+ channels activation. Finally, the comparison of the two cell types suggests that the PM and the ER permeabilization thresholds are affected by the sizes of the cell and the ER. In conclusion, this study demonstrates that mu sPEF, which are easier to control than nsPEF, can permeabilize internal membranes. Besides, mu sPEF interaction with either the PM or ER, can be an efficient tool to modulate the cytosolic calcium concentration and study Ca2+ roles in cell physiology

Janis-Newman-Winicour and Wyman solutions are the same

We show that the well-known most general static and spherically symmetric exact solution to the Einstein-massless scalar equations given by Wyman is the same as one found by Janis, Newman and Winicour several years ago. We obtain the energy associated with this spacetime and find that the total energy for the case of the purely scalar field is zero.Comment: 9 pages, LaTex, no figures, misprints corrected, to appear in Int. J. Mod. Phys.

Cosmology with two compactification scales

We consider a (4+d)-dimensional spacetime broken up into a (4-n)-dimensional Minkowski spacetime (where n goes from 1 to 3) and a compact (n+d)-dimensional manifold. At the present time the n compactification radii are of the order of the Universe size, while the other d compactification radii are of the order of the Planck length.Comment: 16 pages, Latex2e, 7 figure

Technological and theoretical aspects for testing electroporation on liposomes

Recently, the use of nanometer liposomes as nanocarriers in drug delivery systems mediated by nanoelectroporation has been proposed. This technique takes advantage of the possibility of simultaneously electroporating liposomes and cell membrane with 10-nanosecond pulsed electric fields (nsPEF) facilitating the release of the drug from the liposomes and at the same time its uptake by the cells. In this paper the design and characterization of a 10 nsPEF exposure system is presented, for liposomes electroporation purposes. The design and the characterization of the applicator have been carried out choosing an electroporation cuvette with 1 mm gap between the electrodes. The structure efficiency has been evaluated at different experimental conditions by changing the solution conductivity from 0.25 to 1.6 S/m. With the aim to analyze the influence of device performances on the liposomes electroporation, microdosimetric simulations have been performed considering liposomes of 200 and 400 nm of dimension with different inner and outer conductivity (from 0.05 to 1.6 S/m) in order to identify the voltage needed for their poration