Immobilization of lipase on Na-montmorillonite and modified montmorillonite: Investigation of biocatalytic activity of immobilized lipases in biodiesel production from waste cooking oil

In this paper, the immobilization of lipase from Candida rugosa on hydrophilic Na-montmorillonite (MT) and hydrophobic modified montmorillonite (MTS) is investigated. Hydrophobic MTS was prepared by changing the nature of hydrophilic MT with cetrimonium bromide surfactant. The enzymatic activity of lipase immobilized on Na-montmorillonite (LMT) and lipase immobilized on modified montmorillonite (LMTS) was investigated in the production of biodiesel from waste cooking oil. The support modification and enzyme immobilization were evaluated by BET, XRD, and SEM techniques. The effects of temperature, time, water content, and the molar ratio of methanol to oil on biodiesel yield were also investigated. The results showed that the activity of LMTS was much better than LMT. Under optimal conditions, the biodiesel yield produced by LMTS was about 86.4%. LMTS showed good storage stability, and after 30 days of storage at 4 ℃, the biodiesel yield was about 64.2%, while the biodiesel yield of free lipase (FL) was about 57.1%. In addition, LMTS had good reusability compared to LMT, and the biodiesel yield after 10 cycles was about 51.6%

Antioxidant, antibacterial, and keratinocyte cell growth stimulation of herbal extracts used in traditional medicine in IRAN

Abstract Backgrounds and aims: Recently, herbal extracts attracted attention in various medical fields

Enhanced anticancer potency of hydroxytyrosol and curcumin by PLGA-PAA nano-encapsulation on PANC-1 pancreatic cancer cell line

Baradaran, Behzad/0000-0002-8642-6795; Goleij, Pouya/0000-0002-2213-497XWOS:000612051700001PubMed: 33496383Many chemotherapeutic regimens have been investigated for advanced unresectable and metastatic pancreatic cancer (PC), but with only minimal improvement in survival and prognosis. Here, we investigated anti-cancer function of free and nano-encapsulated hydroxytyrosol (Hyd) and curcumin (Cur), and its combinations (Hyd-Cur) on PANC-1 cell line. The poly lactide-co-glycolide-co-polyacrylic acid (PLGA-co-PAA) nano-encapsulated Hyd and Cur were synthesized, and MTT assay was performed to evaluate cytotoxic effects of free and nano-encapsulated Hyd, Cur, and Hyd-Cur. Effects of free and nano-encapsulated Hyd, Cur, and Hyd-Cur were evaluated on viability, migration, morphological alterations, colony formation, and apoptosis on PANC-1 cells. We observed that free and nano-encapsulated Hyd, Cur, and Hyd-Cur significantly increased apoptosis rates as well as significantly decreased viability, migration, and colony formation in PANC-1 cells. According to our results, Hyd-Cur combination and nano-encapsulation therapy exerts more profound apoptotic and anti-proliferative effects on PANC-1 cells than free Hyd or Hyd monotherapy