The transcriptional regulator GalR self-assembles to form highly regular tubular structures

The Gal repressor regulates transport and metabolism of D-galactose in Escherichia coli and can mediate DNA loop formation by forming a bridge between adjacent or distant sites. GalR forms insoluble aggregates at lower salt concentrations in vitro, which can be solubilized at higher salt concentrations. Here, we investigate the assembly and disassembly of GalR aggregates. We find that a sharp transition from aggregates to soluble species occurs between 200 and 400 mM NaCl, incompatible with a simple salting-in effect. The aggregates are highly ordered rod-like structures, highlighting a remarkable ability for organized self-assembly. Mutant studies reveal that aggregation is dependent on two separate interfaces of GalR. The highly ordered structures dissociate to smaller aggregates in the presence of D-galactose. We propose that these self-assembled structures may constitute galactose-tolerant polymers for chromosome compaction in stationary phase cells, in effect linking self-assembly with regulatory function

Preventing Radicalization and Discrimination in Aarhus

Work in this area began as a pilot project in 2007. The aim of the project was to prevent the radicalization - political as well as religious - of young people thereby promoting safety and well-being. On the 1st of January 2011, the project, its operations and leadership were passed over to SSP Aarhus. (SSP is an alliance between Social Services, Schools and The Police

Når kongedømmet bejler til ungdommen

Det saudiske regimes kamp for ny legitimitet efter det arabiske forår: En appel til ungdomsgenerationen igennem brug af retorik og symboler.</jats:p

Forword. Jørgen Bæk Simonsen 70 years

Foror

Indledning. Jørgen Bæk Simonsen 70 år

Forord</jats:p