Effect of triple drug antiretroviral therapy on CD4+ count in pregnant women with HIV and prevention of parent to child transmission

Background: India has moved from single drug Antiretroviral Therapy (ART) in 2002 to triple drug ART in 2013 to prevent parent to child transmission of HIV. The aim of the study was to know the effects of triple drug ART on maternal CD4+ count and prevention of HIV transmission to baby along with its adherence, side effects and pregnancy outcome.Methods: A prospective study wsas done in Safdarjung Hospital, New Delhi on 40 HIV positive pregnant women who received single dose combination of triple drug ART. CD4+ count, LFT and KFT were done before beginning of ART and repeated after 6 months of ART. The infants received nevirapine prophylaxis and HIV status was determined by DBS PCR at 6 weeks.Results: The median CD4+ count was 317 and 397 pre and post ART for 6 months respectively (p value<0.001. Low birth weight (LBW) was seen in 43.59% which was statistically significant but confounded as 76.4% of these babies were preterm. 23.08% of babies had an APGAR of < 7 at 1 minute, out of which 77.7% were preterm. Nine out of 39 infants (one had abortion) needed NICU admission. Only one baby (2.56%) was HIV positive who died at 4 months of age due to pneumonia. There was no defaulter and no statistically significant changes in LFT and KFT after 6 months of ART.Conclusions: Triple drug ART offers greater convenience improves fetomaternal outcome and minimize the risk of HIV transmission from mother to child

A Compendium of Key Climate Smart Agriculture Practices in Intensive Cereal Based Systems of South Asia

CSA initially proposed by FAO in 2010 at “The Hague Conference on Agriculture, Food Security and Climate Change (CC)”, to address the need for a strategy to manage agriculture and food systems, under climate change. The CSA by its original proponents describes the three objectives; i) sustainably increasing agricultural productivity to support equitable increases in incomes, food security and development; ii) adapting and building resilience to climate change from the farm to national levels; and iii) developing opportunities to reduce GHG emissions from agriculture compared with past trends. Since then, these three objectives (in short food security, adaptation and mitigation) are designated as the three “pillars” (or criteria) of CSA within the agricultural science and development communities. Climate Smart (Sustainable Management of Agricultural Resources and Techniques) Agriculture is an approach of crop production, which deals with the management of available agricultural resources with latest management practices and farm machinery, under a particular set of edaphic and environmental conditions. It works to enhance the achievement of national food security and Sustainable Development Goals (SDGs). CSA is location specific and tailored to fit the agro-ecological and socio-economic conditions of a location. CSA may be defined as “agriculture that sustainably increases productivity, resilience (adaptation), reduces/removes greenhouse gases (mitigation), and enhances achievement of national food security and development goals.” Therefore, if CSA implemented at right time with required resources, techniques and knowledge in a particular typological domain, will lead towards food security while improving adaptive capacity and mitigating potential for sustainable agriculture production

Antiplasmodial activity of silver nanoparticles: A novel green synthesis approach

Objective: To synthesize silver nanoparticles using silver nitrate by a green technique which involves different compositions of aqueous leaf extracts of Azadirachta indica (neem) and Ocimum sanctum (tulsi). Methods: Their shape and size were determined using transmission electron microscopy and UV-visible spectroscopy. Their antiplasmodial activity was studied using the malarial parasite strain (Plasmodium falciparum, 3D7). The parasite strain (3D7) was collected and revived in vitro using Trager and Jensen method in RPMI 1640 medium for 7-8 cycles. Half maximal effective concentration values were calculated by nonlinear regression analysis. Results: Transmission electron microscopy results confirmed the formation of silver nanoparticles with size ranging from 4.74-39.32 nm and their size differs by varying the concentrations from 20% to 100% of neem extract in neem and tulsi extracts. It was observed that samples B and C showed half maximum effective concentration of about 0.3 μ M. Conclusions: It can be easily established that the aqueous leaf extracts of neem and tulsi in combination can be a good source for synthesis of silver nanoparticles with small size possessing appreciable antiplasmodial activity

Does climate-smart village approach influence gender equality in farming households? A case of two contrasting ecologies in India

Evidence from climate-smart village (CSV) approach to mainstream climate-smart agriculture (CSA) demonstrates improved productivity, income, and reduced climatic risks. However, its contribution to gender empowerment in diverse farming households is not documented. This study creates a Gender Empowerment Index for climate-smart villages (GEI-CSV) based on four major measurable indicators—political, economic, agricultural, and social. The gender gap was derived by mapping difference in empowerment levels across selected CSVs and non-CSVs. These indicators can be used as a vital tool to understand the process of gender empowerment that can trigger the entry points to achieve gender equality, which is also an important aspect in the adoption of climate-smart agriculture practices (CSAPs). The study measures empowerment at the inter-household and intra-household level across CSVs and non-CSVs from the individual household survey with both female and male members of the same household. This paper provides evidence demonstrating how gender empowerment differs in CSVs and non-CSVs from selected climate-smart villages (community-based approach) in two contrasting ecologies and socio-economic settings of India. The study documents the existing gender gap in CSVs and non-CSVs across India’s western (Haryana) and eastern (Bihar) Indo-Gangetic Plains (IGP). Irrespective of CSVs and non-CSVs, considerable differences in outlook and gender gap were observed between Bihar and Haryana. Both women and men in Bihar are less empowered than they are in Haryana. High empowerment level in CSVs than non-CSVs shows that the concept of CSVs has brought a change towards knowledge and capacity enhancement of both women and men farmers promoting gender equality in farming households with a varying scope of interventions made and required for scaling CSAPs across the diversity of farming households

How profitable climate smart agricultural practices are? Voice of farmers from rice-wheat ecologies

Farm record keeping can provide a look into the health of farm business, its profitability, and a snapshot in time of its present equity. However, farmers in developing country seldom maintain written records of farm operations. In many cases, farmers consider it worthless exercise. They feel overwhelmed by record keeping because it takes time, a change in behavior and for some, the requirement to learn a new skill. This study assesses the role of record keeping, particularly among women and youth using data collected in afarmers participatory research undertaken under the CGIAR Research Program on Climate Change, Agriculture and Food Security (CCAFS). Farmers (both male and female) in climate smart villages (CSVs) of Haryana were acquainted with farm Lekha Jokha (farm budgeting) booklet designed by CIMMYT-CCAFS partners and training to fill the information was given by the field staff including male and females (CIMMYT-CCAFS 2014). The study was undertaken during 2014-15 and 2015-16. Based on the data recorded through Lekha Jokha, the study also estimated the adoption and economics of different climate smart agricultural practices (CSAPs). The farm budgeting found to be very effective tool for increased awareness among women and men farmers especially youth which lead to accelerated adoption of CSAPs. With adoption of CSAPs proven reduced climatic risk was observed in terms of yield penalty, income and distress. The results identifies a platform for social inclusive development in agriculture to promote evidence based informed policy decisions for investment prioritization

Computationally validated SARS-CoV-2 CTL and HTL Multi-Patch vaccines, designed by reverse epitomics approach, show potential to cover large ethnically distributed human population worldwide.

The SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) is responsible for the COVID-19 outbreak. The highly contagious COVID-19 disease has spread to 216 countries in less than six months. Though several vaccine candidates are being claimed, an effective vaccine is yet to come. A novel reverse epitomics approach, 'overlapping-epitope-clusters-to-patches' method is utilized to identify the antigenic regions from the SARS-CoV-2 proteome. These antigenic regions are named as 'Ag-Patch or Ag-Patches', for Antigenic Patch or Patches. The identification of Ag-Patches is based on the clusters of overlapping epitopes rising from SARS-CoV-2 proteins. Further, we have utilized the identified Ag-Patches to design Multi-Patch Vaccines (MPVs), proposing a novel method for the vaccine design. The designed MPVs were analyzed for immunologically crucial parameters, physiochemical properties and cDNA constructs. We identified 73 CTL (Cytotoxic T-Lymphocyte) and 49 HTL (Helper T-Lymphocyte) novel Ag-Patches from the proteome of SARS-CoV-2. The identified Ag-Patches utilized to design MPVs cover 768 overlapping epitopes targeting 55 different HLA alleles leading to 99.98% of world human population coverage. The MPVs and Toll-Like Receptor ectodomain complex shows stable complex formation tendency. Further, the cDNA analysis favors high expression of the MPVs constructs in a human cell line. We identified highly immunogenic novel Ag-Patches from the entire proteome of SARS CoV-2 by a novel reverse epitomics approach and utilized them to design MPVs. We conclude that the novel MPVs could be a highly potential novel approach to combat SARS-CoV-2, with greater effectiveness, high specificity and large human population coverage worldwide