10 research outputs found
Immunohistochemistry of breast cancer and SLN samples.
<p>HCMV proteins were detected in the tissue sections from breast cancer patients (A–D) and tissue sections from sentinel lymph node (G–J) by immunohistochemistry. HCMV IE expression is confined to tumor cells both in breast and SLN specimens (A and G 20x; B and H 40x). (C, D, I, and J), same sections show immunoreactivity to HCMV LA protein (C, I 20x and D, J 40X). Cytokeratin was used as an epithelial marker and positive control (E, K) and omitting primary antibody was used as a negative control (F and L, 20x). Scale bars: (B, D, H, J, 100 <b>µ</b>m), (Others 80 <b>µ</b>m).</p
Immunohistochemical analysis of breast cancer and SLN samples.
<p>The positivity of HCMV was graded into four grades based on the estimated percentage of HCMV IE positive cells in tumors from breast and paired SLN: Grade I (<25% cells positive for HCMV IE), grade II (25–49%), grade III (50–75%) and grade IV (>75%), *n = number.</p
Death of breast cancer patients in relation to known prognostic markers and HCMV IE grade.
<p>Among 73 patients included in this study, 7 deaths were recorded in the SLN-negative (n = 2) and SLN-positive (n = 5) groups (A), 3 of 7 patients were negative for estrogen receptor (B), Progesterone expression were negative in 4 of 7 patients (C). 4 of 7 had high Elston grade (D), HCMV IE grade IV was observed in 6 of 7 patients (E).</p
HCMV IE grade in cancer cells from breast and SLN, and in inflammatory cells from SLN samples.
<p>Patients were divided into 2 groups based on the presence or absence of SLN metastasis in both breast (A) and SLN (B and C) specimens. The positivity of HCMV was graded into four grades based on the percentage of HCMV IE positive cells in tumors from breast and paired SLN: Grade I (<25% cells positive for HCMV IE), grade II (25–49%), grade III (50–75%) and grade IV (>75%). The presence of HCMV IE-infected inflammatory cells in SLN specimen is shown in (C).</p
TaqMan assay standard curve plot (left) and amplification plot (right): For miRNA copy number quantification; standard curve was prepared from ten time dilutions of recombinant plasmid containing hcmv-miR-UL112-3p amplicon as insert, which was used as a template in TaqMan miRNA assays.
<p>TaqMan assay standard curve plot (left) and amplification plot (right): For miRNA copy number quantification; standard curve was prepared from ten time dilutions of recombinant plasmid containing hcmv-miR-UL112-3p amplicon as insert, which was used as a template in TaqMan miRNA assays.</p
Patient characteristics and summarised results.
<p>Patient characteristics and summarised results.</p
The prevalence of hcmv-miR-UL112-3p was determined using a TaqMan miRNA assay, and the seroprevalence of IgG and IgM against HCMV was detected with ELISA assays in all patients and controls.
<p>HC = Healthy Controls, GBM = Glioblastoma multiforme, RA = Rheumatoid Arthritis and DM = Diabetes Mellitus (patients from the DIGAMI-2 cohort).</p
CD4CD28T cells from peripheral blood and synovial fluid show human cytomegalovirus specificity
<p><b>Copyright information:</b></p><p>Taken from "Skewed distribution of proinflammatory CD4CD28T cells in rheumatoid arthritis"</p><p>http://arthritis-research.com/content/9/5/R87</p><p>Arthritis Research & Therapy 2007;9(5):R87-R87.</p><p>Published online 7 Sep 2007</p><p>PMCID:PMC2212553.</p><p></p> The functional capacity of CD4CD28T cells in peripheral blood (PB) and synovial fluid (SF) was investigated after stimulation of mononuclear cells from paired PB and SF from rheumatoid arthritis patients by plate-bound anti-CD3 antibodies. The reactivity of CD4CD28T cells in paired PB and SF to human cytomegalovirus (HCMV) antigens was analysed after stimulation by the pp65 or immediate early (IE) antigens
Skewed distribution of CD4CD28T cells in synovial fluid and peripheral blood
<p><b>Copyright information:</b></p><p>Taken from "Skewed distribution of proinflammatory CD4CD28T cells in rheumatoid arthritis"</p><p>http://arthritis-research.com/content/9/5/R87</p><p>Arthritis Research & Therapy 2007;9(5):R87-R87.</p><p>Published online 7 Sep 2007</p><p>PMCID:PMC2212553.</p><p></p> Paired samples of synovial fluid (SF) and peripheral blood (PB) from 128 rheumatoid arthritis patients were compared for the frequency of CD4CD28T cells by flow cytometry. Frequencies of CD4CD28T cells in SF tend to be higher in patients with large populations in PB. Comparison of the frequency of CD4CD28T cells in SF from two different synovial compartments. Open circle, elbow; open squares, shoulder joints. Frequencies of CD4CD28T cells in SF and PB in patients seronegative and seropositive for human cytomegalovirus (HCMV). Frequencies of CD4CD28T cells in paired PB and SF of patients seropositive for HCMV