<i>FIGL1</i> limits <i>MUS81</i>-dependent CO formation.

<p>A: MLH1 foci number is unchanged in <i>figl1-1</i> compared to wild type. B-C: Anaphase I in <i>mus81</i> (B) and <i>figl1-1 mus81</i> double mutant (C), the latter displays chromosome fragments indicative of unrepaired recombination intermediates. Scale bar = 5μm.</p

The effect of <i>figl1</i> and <i>fancm</i> on recombination in hybrids.

<p>A: CO count in each F2 progeny obtained from parent plants from Columbia-0/Landsberg (Col/Ler) F1 hybrids. Means and SD are indicated. n.s.: not significant; *** indicates significant difference, T-Test p<0,001. B: Recombination frequency (in cM/Mb) along chromosome I compared to wild type for each genotype. Difference in recombination frequency was tested along the genome on ~5Mb intervals (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005369#sec014" target="_blank">methods</a>). See also <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005369#pgen.1005369.s005" target="_blank">S5A Fig</a>. C: Genetic distances (in cM) measured from tetrad analysis in a series of intervals, in Col/Ler F1 hybrids. All genotypes on all intervals are significantly different from wild type (Z-test, p<0.03), except when noted (n.s.: not significant).</p

The dynamics of DMC1 and RAD51 are modified in <i>figl1</i>.

<p>A and C: Number of RAD51 and DMC1 (respectively) foci count per positive cell throughout prophase in both wild type and <i>figl1-1</i> mutant. n.s.: not significant; *** T-test p<0,001. B: Illustration of RAD51immuno-localization at leptotene in wild type and <i>figl1-1</i> mutant, with the axis protein ASY1used as a counterstain. D: Illustration of DMC1immuno-localization at leptotene in wild type and <i>figl1-1</i> mutant, with the REC8 cohesin used as a counterstain. The same exposure and treatment parameters have been applied to all images of both wild type and <i>figl1-1</i>. E: DMC1 foci count in pachytene cells (*** T-test p<0,001). ZYP1 staining was used as a marker for full synapsis, indicative of the pachytene stage. F: Illustration of DMC1 immuno-localization at pachytene, with ZYP1 as a counterstain.</p

Mutations in <i>FIGL1</i> restores bivalent formation in <i>zmm</i> mutants.

<p>A: Gene model of the <i>FIDGETIN-Like-1</i> gene, exons appear as blue boxes, the conserved domains are indicated in green, red and purple. Black lines represent the position of the point mutations. B: Univalent pairs (red) and bivalents (blue) count of metaphase I male meiocytes in wild type, <i>zmm</i> mutants (<i>zip4</i>, <i>msh4</i>, <i>msh5</i>, <i>hei10</i> and <i>shoc1</i>) and in <i>zmm figl1</i> double mutants, as well as <i>figl1</i> single mutants and <i>figl1-1 spo11</i> double mutants. All genotypes are in a Columbia-0 background, except for <i>msh4 figl1-12</i> and <i>msh4 figl1-13</i> which are in a Landsberg erecta background (Ler).</p