2 research outputs found
The importance of markers HLA6 and CD68 in placenta tissues of recurrent pregnancy loss
Introduction: Recurrent pregnancy loss of unknown etiology is correlated with immunological factors during pregnancy. Changes in leukocyte subpopulations and HLA (Human Leukocyte Antigen) expression take place in pregnant uterus on both decidua basalis and decidua parietalis in order to carry the semiallogenic embryo. These changes affect the pregnancy course.Objective: Our research is focused to enlighten the immunological changes that take place in the uterus of women with recurrent abortions of unknown etiology during first trimester of pregnancy.Materials and methods: The miscarriage group was obtained from 25 women who miscarried between the ages of 35 to 42 years and controls consisted of 25 healthy women between the ages of 27 to 39 years, who had electively terminated their pregnancies during first trimester of pregnancy. The abortion was processed and specimens taken were studied, using immunohistochemical methods. Specimens were taken from decidua basalis and decidua parietalis. Monoclonal antibodies were used against HLAG (Human Leukocyte Antigen G) and CD68 (Cluster of Differentiation 68). The results were statistically analysed with Mann-Whitney test.Results: HLA-G expression in decidua basalis from miscarriage group was found decreased.CD68 + cell expression was found increased in both deciduas from the miscarriage group.Conclusion: The immunological profile of women with recurrent miscarriage is quite different comparing with controls. A possible role of CD68+cells in RPL was observed .Changes in HLA-G expression was observed
Immunohistochemical study of immunological markers: HLAG, CD16, CD25, CD56 and CD68 in placenta tissues in recurrent pregnancy loss
Introduction: Recurrent pregnancy loss
(RPL) of unknown etiology is correlated with
immunological alterations during pregnancy. Normally,
changes in leukocyte subpopulations and HLA
expression take place in pregnant uterus in order to
tolerate the semi-allogenic embryo. Objective: Our
research tries to enlighten the immunological changes
that take place in the uterus of women with recurrent
abortions of unknown etiology during first trimester of
pregnancy. Materials and methods: The miscarriage
group was obtained from 25 women who miscarried
between the ages of 35 to 42 years and controls
consisted of 25 healthy women between the ages of 27 to
39 years, who had electively terminated their
pregnancies during the first trimester of pregnancy. The
abortion was processed and specimens taken were
studied using immunohistochemical methods.
Specimens were taken from decidua basalis and decidua
parietalis. Monoclonal antibodies were used against
HLAG (Human Leukocyte Antigen G), CD68( Cluster of
Differentiation 68), CD56, CD16 and CD25. The results
were statistically analysed with Mann-Whitney test.
Results: HLA-G expression in decidua basalis from
miscarriage group was found to be decreased. CD25+
cell expression was found to be invariable in deciduas
from both groups. CD16+ cell and CD68 + cell
expression was found to be increased in deciduas from
the miscarriage group. CD56+ cell expression was found
to be increased in decidua parietalis from miscarriage
group. Conclusion : Several differences in the
immunological profile of deciduas from RPL group were
observed. Changes in feto-protective HLA-G expression
and a possible implication of macrophages and NK cells
were found