Pairwise relationships between mtDNA, microsatellites and geographical distances in the Rhone drainage.

<p>Pairwise relationship between Fst/(1−Fst) (genetic distance) vs. geographical distances (km) using microsatellites (A) and mtDNA (B) or between microsatellites vs. mtDNA genetic distances in the Rhone drainage. The correlation coefficient (R) and the P-value (P) obtained from Mantel test are reported for each bivariate analysis.</p

Molecular datations from mtDNA sequences.

a<p>Nodes of the MJ network used to estimate TMRCAs from ρ statistic (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034423#pone-0034423-g003" target="_blank">Figure 3</a>).</p

Sampled <i>Telestes</i> taxa, population locations and sample sizes.

a<p>see main text for abbreviations.</p

Phylogenetic and multivariate analyses of microsatellites data.

<p>Unrooted Maximum Likelihood tree based on microsatellites Dc distances (A) and FCA scatter plots for <i>T. s. souffia</i> population samples (B) and population samples representative of <i>Telestes</i> genus (C).</p

Location of the sampled <i>Telestes</i> populations with emphasize on the Rhone and French Mediterranean basins.

<p>For samples ID, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034423#pone-0034423-t001" target="_blank">Table 1</a>.</p

Bayesian clustering analyses of microsatellites using Structure.

<p>Estimated population structure for population samples representative of <i>Telestes</i> genus (A) and <i>T. s. souffia</i> (B). “*” indicates K values retained from Δ<i>K </i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034423#pone.0034423-Evanno1" target="_blank">[67]</a> and Δ<i>Fst </i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034423#pone.0034423-Campana1" target="_blank">[64]</a> tests and when maximizing the posterior probability value <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034423#pone.0034423-Pritchard1" target="_blank">[60]</a>. Between parentheses, the number of convergent runs associated to the structure displayed.</p

Phylogenetic analyses of mtDNA sequences.

<p>A. Phylogenetic relationships among haplotypes of <i>Telestes</i> hypothesized from Bayesian analysis using 1232 bp of mtDNA. Posterior probability*100 are indicated, with “*” corresponding to pp = 0.1 while “-” are used to indicate pp<0.5. B. Bayesian time calibrated maximum clade credibility tree using relaxed clock with the same dataset but selected terminals representing major lineages. The calibration point used was the divergence between <i>T. pleurobipunctatus</i> and <i>T. alfiensis</i>. Posterior probabilities are indicated in red on the upper left of the nodes while the modes of the posterior distributions of the age of the nodes are indicated in Myr on the lower left in black. 95% CI are indicated with blue bars centered on the nodes.</p

Median-Joining network of the <i>T. souffia</i> mitochondrial haplotypes.

<p>Population samples ID detailed in haplotypes charts when necessary.</p

Time frame of the Quaternary period and the TMRCA obtained for <i>T. souffia</i> lineages.

<p>The mean and 95% CI maximum interval of the TMRCA obtained for <i>T. souffia</i> lineages using mtDNA sequences and MIS are indicated. A. <i>T. souffia</i> (Rhone and Danube); B. <i>T. souffia</i> (Danube only); C. <i>T. s. agassii</i> 1 vs. <i>T. s. agassii</i> 2; D. <i>T. s. souffia</i>; E. <i>T. s. agassii</i> 1; F. <i>T. s. agassii</i> 2. Green “*”, means of TMRCA obtained from BEAST; red “*”, means of TMRCA obtained from the ρ statistic methodology; blue “*”, means of TMRCA obtained from BSP methodology.</p