Lagochilascaris minor: antibody production in experimentally infected mice Lagochilascaris minor: produção de anticorpos em camundongos experimentalmente infectados

Lagochilascaris minor is the causative agent of lagochilascariosis, a disease that affects the neck region and causes festering abscesses, with eggs, adult parasites and L3/L4 larvae within the purulent exudates. Today, mice are considered to be intermediate hosts for the parasite. C57BL/6 mice produce immunoglobulin IgM, IgA and IgG against the crude extract of the parasite; on the other hand, antibodies produced against the secreted/excreted antigens of Lagochilascaris minor present lower levels of IgM, IgA and IgG. This is the first description of antibody detection against different antigens of Lagochilascaris minor.<br>Lagochilascaris minor é o agente etiológico da lagochilascariose, uma doença que afeta a região do pescoço causando abscessos exudativos com presença de ovos, parasitos adultos e larvas nos de exudatos purulentos. Hoje em dia, camundongos são considerados os hospedeiros intermediários para o parasita. Camundongos C57BL/6 produziram imunoglobulinas IgM, IgA e IgG contra o extrato bruto do parasita; por outro lado, anticorpos produzidos contra os antígenos secretados/excretados de Lagochilascaris minor apresentaram níveis mais baixos de IgM, IgA e IgG. Esta é a primeira descrição da detecção de anticorpos contra diferentes antígenos de Lagochilascaris minor

Comparing the profile of anti-immunoglobulin G in murine immunized with human IgG associated with different adjuvants

v.6, n.1, p. 44-50, jan./abr. 2009.Submitted by Marlene Santos ([email protected]) on 2013-09-17T19:21:22Z No. of bitstreams: 1 5860-22029-2-PB.pdf: 106180 bytes, checksum: 3f49b60695e52dc526ddbba926f8140f (MD5)Approved for entry into archive by Claudia Moura ([email protected]) on 2013-10-23T17:24:40Z (GMT) No. of bitstreams: 1 5860-22029-2-PB.pdf: 106180 bytes, checksum: 3f49b60695e52dc526ddbba926f8140f (MD5)Approved for entry into archive by Claudia Moura ([email protected]) on 2013-10-23T17:27:06Z (GMT) No. of bitstreams: 1 5860-22029-2-PB.pdf: 106180 bytes, checksum: 3f49b60695e52dc526ddbba926f8140f (MD5)Made available in DSpace on 2013-10-23T17:27:26Z (GMT). No. of bitstreams: 1 5860-22029-2-PB.pdf: 106180 bytes, checksum: 3f49b60695e52dc526ddbba926f8140f (MD5) Previous issue date: 2009-03Pró-Reitoria de Pesquisa e Pós-Graduação da Universidade Federal de Goiás.Adjuvantes são substâncias associadas aos antígenos que aumentam ou modificam a resposta imune. Este trabalho constitui uma comparação do perfil de anticorpos em grupos de murinos imunizados com IgG humana associada ou não a adjuvantes, através da técnica de Enzyme Linked Immunosorbent Assay (ELISA) no 28o e 40º dia após esquema de imunização previamente delineado. Os adjuvantes avaliados nesse estudo foram o hidróxido de alumínio e o adjuvante de Freund. Os resultados demonstraram que no 28º dia os animais imunizados com adjuvantes exibiram valores de absorbância mais altos em comparação com animais imunizados sem adjuvantes e os murinos imunizados com hidróxido de alumínio e adjuvante de Freund apresentaram valores de densidade ótica semelhantes. Em contradição, no 40º dia nos três grupos de camundongos estudados, o perfil de anticorpos anti – Ig G humana foram análogos.Adjuvants are substances associated with antigens that increase or modify the imune response. This work/study is a comparison of the profile of murine antibodies in groups immunized with human IgG associated or not with adjuvants, using the Enzyme Linked Immunosorbent Assay (ELISA) technique in the 28th and 40th days after immunization. The adjuvants evaluated in this work/study were the aluminium hydroxide and Freund’s adjuvant. The results showed that in 28th day the mice immunizad with adjuvants exhibited higher values of absorbance compared with the animals immunized without adjuvants. However, it was found that the murine immunized with aluminium hydroxide and Freund’s adjuvant showed similar values of optical density. In contradiction, in the 40th day the groups of mice examined showed analogues profiles of anti-human IgG

NEOTROPICAL CARNIVORES: a data set on carnivore distribution in the Neotropics

Mammalian carnivores are considered a key group in maintaining ecological health and can indicate potential ecological integrity in landscapes where they occur. Carnivores also hold high conservation value and their habitat requirements can guide management and conservation plans. The order Carnivora has 84 species from 8 families in the Neotropical region: Canidae; Felidae; Mephitidae; Mustelidae; Otariidae; Phocidae; Procyonidae; and Ursidae. Herein, we include published and unpublished data on native terrestrial Neotropical carnivores (Canidae; Felidae; Mephitidae; Mustelidae; Procyonidae; and Ursidae). NEOTROPICAL CARNIVORES is a publicly available data set that includes 99,605 data entries from 35,511 unique georeferenced coordinates. Detection/non-detection and quantitative data were obtained from 1818 to 2018 by researchers, governmental agencies, non-governmental organizations, and private consultants. Data were collected using several methods including camera trapping, museum collections, roadkill, line transect, and opportunistic records. Literature (peer-reviewed and grey literature) from Portuguese, Spanish and English were incorporated in this compilation. Most of the data set consists of detection data entries (n = 79,343; 79.7%) but also includes non-detection data (n = 20,262; 20.3%). Of those, 43.3% also include count data (n = 43,151). The information available in NEOTROPICAL CARNIVORES will contribute to macroecological, ecological, and conservation questions in multiple spatio-temporal perspectives. As carnivores play key roles in trophic interactions, a better understanding of their distribution and habitat requirements are essential to establish conservation management plans and safeguard the future ecological health of Neotropical ecosystems. Our data paper, combined with other large-scale data sets, has great potential to clarify species distribution and related ecological processes within the Neotropics. There are no copyright restrictions and no restriction for using data from this data paper, as long as the data paper is cited as the source of the information used. We also request that users inform us of how they intend to use the data