Accidental and late parasitological diagnosis of Leishmania sp. in a dog from a low disease transmission area of Brazil: a case report

Canine Leishmaniasis diagnosis must be fast and accurate since dogs are urban reservoirs of the disease and earlier therapeutic intervention is more clinically effective. However, this still represents a challenge, particularly in low transmission areas. The present report describes the difficulties of clinical suspicion and the late diagnosis of a dog infected with Leishmania sp

Modification of the Alere GIARDIA Ag TEST immunochromatography KIT methodology for its use in frozen fecal sediment of dogs and cats

ABSTRACT Giardia duodenalis is a worldwide intestinal parasite and is one of the most frequent protozoa species infecting dogs and cats. This study aimed to modify the methodology of Alere GIARDIA Ag TEST KIT for its use in frozen fecal sediments with different storage times in a freezer (-20°C), thus expanding the range of use of this methodology. One hundred fecal sediments from dogs (n=50) and cats (n=50) previously examined by optical microscopy for Giardia cysts were selected for this study. The agreement between the modified immunochromatography and microscopy results was calculated by Kappa coefficient. To evaluate the performance of the modified immunochromatography assay on samples with different storage time, the fecal sediments were divided into three groups according to the time of storage in a freezer: (a) ≤ 1 year (n=37); (b) > 1 year and ≤ 3 years (n=39); (c) > 10 years (max. 13 years) (n=24). The results obtained by the modified immunochromatography assay demonstrates a higher sensitivity of this technique when compared with microscopy, regardless of the frozen storage time. These results allow for the use of this methodology in a greater scope of analysis, especially in frozen fecal sediment triage in sample collections, enabling epidemiological and comparative analysis along different decades

Accidental and late parasitological diagnosis of Leishmania sp. in a dog from a low disease transmission area of Brazil: a case report

Submitted by Janaína Nascimento ([email protected]) on 2019-10-02T12:25:57Z No. of bitstreams: 1 ve_Spinelli_Renan_etal_INI_2019.pdf: 224266 bytes, checksum: 8944b82f3ac9165971852e002511661e (MD5)Approved for entry into archive by Janaína Nascimento ([email protected]) on 2019-10-02T14:14:19Z (GMT) No. of bitstreams: 1 ve_Spinelli_Renan_etal_INI_2019.pdf: 224266 bytes, checksum: 8944b82f3ac9165971852e002511661e (MD5)Made available in DSpace on 2019-10-02T14:14:19Z (GMT). No. of bitstreams: 1 ve_Spinelli_Renan_etal_INI_2019.pdf: 224266 bytes, checksum: 8944b82f3ac9165971852e002511661e (MD5) Previous issue date: 2019Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.Laboratório Clínico Veterinário Flávia Uchôa. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos, Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Departamento de Ciências Biológicas Laboratório de Imunodiagnóstico. RJ, Brasil.Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.Canine Leishmaniasis diagnosis must be fast and accurate since dogs are urban reservoirs of the disease and earlier therapeutic intervention is more clinically effective. However, this still represents a challenge, particularly in low transmission areas. The present report describes the difficulties of clinical suspicion and the late diagnosis of a dog infected with Leishmania sp

Ocorrência e fatores de risco da infecção por Toxoplasma gondii em suínos criados e abatidos na região do Triângulo Mineiro, Minas Gerais, Brasil

Submitted by Sandra Infurna ([email protected]) on 2018-02-06T12:01:41Z No. of bitstreams: 1 mariaregina_amendoeira_etal_IOC_2017.pdf: 917033 bytes, checksum: 74384c0b1588811f16d472eb6cf3a6a7 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2018-02-06T12:14:56Z (GMT) No. of bitstreams: 1 mariaregina_amendoeira_etal_IOC_2017.pdf: 917033 bytes, checksum: 74384c0b1588811f16d472eb6cf3a6a7 (MD5)Made available in DSpace on 2018-02-06T12:14:56Z (GMT). No. of bitstreams: 1 mariaregina_amendoeira_etal_IOC_2017.pdf: 917033 bytes, checksum: 74384c0b1588811f16d472eb6cf3a6a7 (MD5) Previous issue date: 2017Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Parasitologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozoonoses. Rio de Janeiro, RJ. Brasil.Universidade Federal de Uberlândia. Faculdade de Medicina Veterinária. Uberlândia, MG, Brasil.Sem afiliaçãoUniversidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Parasitologia. Niterói, RJ, Brasil.Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Parasitologia. Niterói, RJ, Brasil.Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Parasitologia. Niterói, RJ, Brasil.Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Parasitologia. Niterói, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozoonoses. Rio de Janeiro, RJ. Brasil.The Triângulo Mineiro region from Minas Gerais state, is an important meat-exporting region of Brazil and data about Toxoplasma gondii infection in pigs raised and slaughtered in this area are scarce. Therefore, the aim of this study was to evaluate the occurrence of T. gondii in swine and establish the risk factors associated with the infection. Samples were collected from 600 pigs raised under intensive system in farms located at three different counties (Carmo do Paranaíba, Patrocínio and Perdizes). The samples were submitted to indirect hemagglutination antibody test with dilution of 1:32 and to indirect immunofluorescence antibody test with a cutoff of 1:64. The occurrence of positive pig was 3.3% (n=20) and 51.8% (n=311) respectively. A significant difference was observed between toxoplasmatic infection and factors such as lineage, animal origin, size of the farm, collective raising with others species, presence of rodents and type of water offered (p≤0.05). There was no difference between gender and the farm goals. The results demonstrated an occurrence of anti-T. gondii antibodies higher than expected for intensive pig raising system on the studied area, which could indicate a possible sanitary management problem on the studied proprieties. Improvements on the raising techniques are necessary to reduce T. gondii infection sources

Occurrence of Toxoplasma gondii and risk factors for infection in pigs raised and slaughtered in the Triângulo Mineiro region, Minas Gerais, Brazil

ABSTRACT: The Triângulo Mineiro region from Minas Gerais state, is an important meat-exporting region of Brazil and data about Toxoplasma gondii infection in pigs raised and slaughtered in this area are scarce. Therefore, the aim of this study was to evaluate the occurrence of T. gondii in swine and establish the risk factors associated with the infection. Samples were collected from 600 pigs raised under intensive system in farms located at three different counties (Carmo do Paranaíba, Patrocínio and Perdizes). The samples were submitted to indirect hemagglutination antibody test with dilution of 1:32 and to indirect immunofluorescence antibody test with a cutoff of 1:64. The occurrence of positive pig was 3.3% (n=20) and 51.8% (n=311) respectively. A significant difference was observed between toxoplasmatic infection and factors such as lineage, animal origin, size of the farm, collective raising with others species, presence of rodents and type of water offered (p≤0.05). There was no difference between gender and the farm goals. The results demonstrated an occurrence of anti-T.gondii antibodies higher than expected for intensive pig raising system on the studied area, which could indicate a possible sanitary management problem on the studied proprieties. Improvements on the raising techniques are necessary to reduce T. gondii infection sources