Calderon and the rewriting of the titles of comedies

If rewriting is one of the identity signs of Caldern's theatre, it is useful to start from the beginning and examine the occasional revision of his works' titles, because this paratext is the principal presentation card of every text. In this way, this work analyzes the cases in where the title changes between two versions of the play (Amor, honor y poder, La devocin de la cruz, El mayor monstruo del mundo, Guardate del agua mansa) in order to enquire the fonction of this variation, which could mean a new authorial intent and new perspectives of construction, approach and meaning.If rewriting is one of the identity signs of Calderón’s theatre, it is useful to start from the beginning and examine the occasional revision of his works’ titles, because this paratext is the principal presentation card of every text. In this way, this work analyzes the cases in where the title changes between two versions of the play (Amor, honor y poder, La devoción de la cruz, El mayor monstruo del mundo, Guárdate del agua mansa) in order to enquire the fonction of this variation, which could mean a new authorial intent and new perspectives of construction, approach and meaning.Si la reescritura es una de las marcas de identidad de la dramaturgia de Calderón, conviene comenzar por el principio y examinar la revisión ocasional que lleva a cabo sobre sus títulos, toda vez que este paratexto es la principal tarjeta de presentación de todo texto. De este modo, en este trabajo se examinan los casos en los que varía el título entre dos versiones de una comedia (Amor, honor y poder, La devoción de la cruz, El mayor monstruo del mundo, Guárdate del agua mansa) para indagar en la función de este cambio, que puede significar una nueva voluntad autorial y variaciones de construcción, enfoque y sentido

GPER overexpression in cervical cancer versus premalignant lesions: Its activation induces different forms of cell death

Background: The effect of estrogen has been traditionally studied through the modulation of its alpha and beta nuclear receptors; however, the G Protein-Coupled Estrogen Receptor (GPER) has been recently involved in the pathology of numerous tumors. Although the study of GPER in cervical cancer has begun, its contribution still remains to be completely evaluated. Objective: The purpose of this work was to determine the expression of this receptor in different degrees of cervical lesions and whether the stimulation with its specific agonist (G-1) modulated mechanisms of cell survival or cell death in cervical cancer cells. Methods: Sections of 44 formalin-fixed paraffin-embedded blocks from patients were analyzed by automated immunohistochemistry. After the stimulation with G-1, proliferation was evaluated by the xCELLigence technology, the integrity of the mitochondrial membrane permeability by MitoCapture™ fluorescence staining, apoptosis by flow cytometry, and senescence by the senescence-associated β-galactosidase kit. Results: GPER was widely expressed in cervical cancer but not in its precursor lesions. The staining was predominantly cytoplasmic, although it was also important in the nucleus of the epithelial cells. G-1 inhibited proliferation, decreased the mitochondrial permeability, and increased the percentage of apoptosis in SiHa, HeLa, and C-33A. Only in C-33A, an increase of the cells in necrosis was observed, whereas SiHa was the only cell line in which senescence was evidenced. Conclusion: GPER is a receptor associated with cervical cancer that inhibits the growth and induces different mechanisms of death in cells derived from uterine cervical cancer. It suggests that GPER can be considered a pharmacological target that prevents the development of cervical carcinogenesis.Fil: Silva, Christian Oscar. Universidad de Guadalajara; MéxicoFil: Leal, Annie R.. Universidad de Guadalajara; MéxicoFil: Ortiz Lazareno, Pablo C.. Instituto Mexicano del Seguro Social; MéxicoFil: Suárez, Luis F. J.. Instituto Mexicano del Seguro Social; MéxicoFil: de Arellano, Adrián R.. Universidad de Guadalajara; MéxicoFil: Lopez Pulido, Edgar I.. Universidad de Guadalajara; MéxicoFil: Barragan, José G. M.. Universidad de Guadalajara; MéxicoFil: Buelna, Margarita M.. Universidad de Guadalajara; MéxicoFil: Rodríguez, Judith R. D.. Hospital Civil de Guadalajara; MéxicoFil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; ArgentinaFil: Muñoz Valle, José F.. Instituto Mexicano del Seguro Social; MéxicoFil: Pereira Suárez, Ana L.. Universidad de Guadalajara; Méxic