Studies of Genetic and Morphological Characteristics of Indonesian Melon (Cucumis melo L. ‘Hikapel’) Germplasm

The abundance of biodiversity in Indonesia is due to variations in germplasm and the diversity of plant species. Due to the high potential and demand for melons, it is necessary to innovate the assembly of superior varieties through a plant breeding approach. Here, we report the identification of phenotypic and genotypic traits with their phenetic relationships to determine the premium characteristics of the new melon cultivar - Hikapel. Observation of morphological characters using observational studies, qualitative descriptive, and quantitative identification at two cultivation locations: (1) Prambanan, Sleman Regency, Yogyakarta, and (2) Pangalengan, Bandung Regency, West Java. Meanwhile, molecular character testing uses the ISSR markers UBC-807, UBC-808, UBC-810, UBC-812, and UBC-825. Morphological character data were analyzed using SPSS one-way ANOVA with LSD and Duncan tests. All data were grouped based on similarity values using cluster analysis of the multi-variate statistical package (MVSP 3.1) to determine the tested kinship relationship between the varieties. The results showed that Hikapel has several diagnostic characters as a new variety, namely: globular shape with cream-colored skin, aromatic fruit, small size, high sweetness level, and short harvest time. The stability and uniformity test results those characters of Hikapel can be maintained at various locations. This research can serve as basic data for developing melon varieties based on genomic data and aims as an initial step in the innovation of Indonesian Agriculture

Detection of Transposon Gene in Lurik Peanuts (Arachis hypogaea var. lurik L.) with AhMITEs Analysis

Peanut (Arachis hypogaea L.) is one of the leading commodities in Indonesia that are consistently growing with high demand. However, its productivity in the current state is relatively few, thus causing dependency on imported products. Developing new varieties is one of the many solutions to these problems. Lurik peanuts are superior local varieties to any other peanuts in terms of productivity and disease resistance. Seed with the purple pattern is this cultivar's special characteristic and main attraction. This study aimed to identify and verify the activity of transposon genes in the seed pattern of Lurik peanuts. This research method was carried out by gene detection and sequencing analysis using PCR-AhMITEs (Arachis hypogaea Miniature Inverted Transposable Elements). The study used the Garuda variety as a comparison due to the absence of seed patterns, and it is a superior variety widely cultivated in Indonesia. Four types of primers used in this study were AhMITE1, AhTE0357, AhTE0391, and AhTE1317. The results revealed that the four primers had a linear relationship that could distinguish Lurik peanuts and Garuda peanuts based on the presence of transposon genes. The sequencing results confirmed that the detected genes were transposons from peanuts, located on chromosome 5 (Arahy.5), chromosome 9 (Arahy.9), chromosome 14 (Arahy.14), and chromosome 19 (Arahy.19). Based on the results of the study, the pattern on Lurik peanuts is an expression of the transposon gene activity

Detection of Transposon Gene in Lurik Peanuts (Arachis hypogaea var. lurik L.) with AhMITEs Analysis

Peanut (Arachis hypogaea L.) is one of the leading commodities in Indonesia that are consistently growing with high demand. However, its productivity in the current state is relatively few, thus causing dependency on imported products. Developing new varieties is one of the many solutions to these problems. Lurik peanuts are superior local varieties to any other peanuts in terms of productivity and disease resistance. Seed with the purple pattern is this cultivar's special characteristic and main attraction. This study aimed to identify and verify the activity of transposon genes in the seed pattern of Lurik peanuts. This research method was carried out by gene detection and sequencing analysis using PCR-AhMITEs (Arachis hypogaea Miniature Inverted Transposable Elements). The study used the Garuda variety as a comparison due to the absence of seed patterns, and it is a superior variety widely cultivated in Indonesia. Four types of primers used in this study were AhMITE1, AhTE0357, AhTE0391, and AhTE1317. The results revealed that the four primers had a linear relationship that could distinguish Lurik peanuts and Garuda peanuts based on the presence of transposon genes. The sequencing results confirmed that the detected genes were transposons from peanuts, located on chromosome 5 (Arahy.5), chromosome 9 (Arahy.9), chromosome 14 (Arahy.14), and chromosome 19 (Arahy.19). Based on the results of the study, the pattern on Lurik peanuts is an expression of the transposon gene activity. © IJASEIT is licensed under a Creative Commons Attribution-Share Alike 4.0 International License

Assessment of maturity stage and stabilityof new Indonesian melon cultivar ‘Melona’ based on ISSR markers and morphological characteristics

Market preferences for horticultural products determine the product’s selling value and absorption. One market criterion often used for the quality of horticultural products is color and maturity level. Color differences can provide a representation of the metabolites contained in a product. This study provides a standard for assessing melons' maturity level, which will be helpful as a benchmark for Indonesian consumer acceptance. In addition, this study also provides information on the stability of the character of the new cultivar 'Melona' from several generations of crosses to obtain superior cultivars. Assessment of maturity level based on color development in 'Melona' is divided into fivestages.In the firstand second stage, fruit was dominated by green color with Increasing fruit volume. The color change begins in the third stage. in the third stage and the following phases, there is an increase in the accumulation of beta-caroteneand characterized by the dominance of orange instead of green.Maximum ripe of ‘Melona’ is characterized by strong yellow lobes, creamy-white interlobes, and orange flesh color. A molecular analysis employingfour ISSR primers (UBC-807, UBC-808, UBC-811, and UBC-824) resulted in high uniformity and low geneticvariationin the hybrid and parent lines genotype. Analysis resulteda total of 42 DNA fragments with only one polymorphic DNA fragment.The polymorphic DNA fragment was 2,300 bp in size and was found in the female accession 'Melona' and the hybrid 'Melona' based on the UBC-808 primer. The numerical phenetic relationship between male, female and hybrid accession melon cultivars is very close(above 97.6%) and indicated that the melona cultivars were stable

Proceedings of the 3rd International Conference on Community Engagement and Education for Sustainable Development

This proceeding contains articles on the various ideas of the academic community presented at The 3rd International Conference on Community Engagement and Education for Sustainable Development (ICCEESD 2022) organized by the Universitas Gadjah Mada, Indonesia on 7th-8th December 2022.  ICCEESD is a biannual forum for sharing, benchmarking, and discussing HEI’s activities in developing Education for Sustainable Development towards community engagement. Education for Sustainability as a teaching strategy for resolving community challenges through formal, informal, or non-formal education is expected to benefit from various community service best practices by academics, researchers, and students. The 3rd ICCEESD has “Strengthening Education for Sustainability Towards Better Community Engagement” as its theme this year. It is expected that the 3rd ICCEESD will provide a forum for the presenters and participants to exchange best practices, policies, and conceptual implementation of Education for Sustainability towards better community engagement and explore ideas to address community needs.  Conference Title: 3rd International Conference on Community Engagement and Education for Sustainable DevelopmentConference Theme: Strengthening Education for Sustainability Towards Better Community EngagementConference Acronyms: ICCEESD 2022Conference Date: 7th-8th December 2022Conference Location: Grand Rohan Jogja Yogyakarta, IndonesiaConference Organizer: Universitas Gadjah Mada, Indonesi