Hepatitis Delta Virus in Patients Referred for Malaria Parasite Test in Ile-Ife, Nigeria

Hepatitis delta virus (HDV) infection is considered the most severe form of human viral hepatitis. The infection only occurs in the presence of a concomitant hepatitis B virus (HBV) and leads to severe liver disease that includes fulminant liver failure and rapid progression to cirrhosis, as well as increased risk of hepatocellular carcinoma (HCC). Diverse data exist on HBsAg carrier rates, but not much is known about the rates of HDV co-infection in cases of HBV infections in Nigeria. More so, HDV has not been detected in people with unknown HBV status or people who had no clinical evidence of liver disease in Nigeria. We therefore determined the prevalence of HBV and HBV/HDV co-infection in patients without clinical evidence of liver disease referred for malaria parasites test from two health institutions in Ile-Ife, Nigeria, using one step HBsAg diagnostic kit (Nova Diagnostic®, USA) and HDV IgM and IgG ELISA kit (Dia.Pro, Italy). Of the 275 patients tested, 38 (13.8 %) were HBsAg positive and 12 (31.6%) of them had antibody to HDV, hence HBV/HDV prevalence in the study population was 4.4 % (12/275). The result of this study shows a significant HBV/HDV prevalence in the study population, and the need for public enlightenment to further improve management. There is also the need to consider HDV screening in patients with no clinical evidence of liver disease. Keywords: HDV, Hepatitis B, co-infection,, ELISA, Nigeri

Hepatitis B Core IgM antibody (anti-HBcIgM) among hepatitis B Surface antigen (HBsAg) negative blood donors in Nigeria

<p>Abstract</p> <p>Background</p> <p>Transfusion associated Hepatitis B virus (TAHBV) continues to be a major problem despite mandatory screening for Hepatitis B surface Antigen (HBsAg). Presence of HBsAg is the common method for detecting hepatitis B infection. Unfortunately, this marker is not detected during the window period of the infection. Nigeria being a developing country cannot afford DNA testing of all collected units of blood which serve as the only possibility of achieving zero risk of transfusion associated HBV. Five different serological makers of hepatitis B virus (HBV) infection were therefore assessed to evaluate the reliability of using HBsAg marker alone in diagnosis of HBV infection among blood donors and to detect the serological evidence of the infection at the window period. This will preclude the possibility of transmitting hepatitis B through transfusion of Hepatitis B surface antigen (HBsAg) negative blood in Nigeria.</p> <p>Methods</p> <p>Between July and August 2009, 92 blood donors were enrolled for the study. The prevalence of 5 different markers of Hepatitis B virus infection was detected using Enzyme Linked Immunosorbent Assay (ELISA). Demographic factors were assessed during the study.</p> <p>Results</p> <p>HBsAg and its antibody (anti-HBs) was detected in 18 (19.6%) and 14(15.2%) of the 92 blood donors respectively. Anti-HBc IgM was found in 12(13.0%) of the 92 blood donors while Hepatitis B envelope antigen (HBeAg) and its antibody (anti-HBe) were detected in 4(8.9%) and 12(26.7%) respectively from 45 donors sampled. HBeAg is a marker of high infectivity and appears after HBsAg. At least one serological marker was detected in 30(32.6%) of the blood donors. Five (5.4%) of the 92 donors had anti-HBc IgM as the only serological evidence of hepatitis B virus infection.</p> <p>Conclusions</p> <p>The result of this study shows that five donors have anti-HBcIgM as the only serological evidence of HBV infection. Inclusion of anti-HBcIgM in routine screening of blood donors in Nigeria should be encouraged. This is the first study to assess anti-HBcIgM in the country.</p

High Prevalence of Anti-HCV Antibodies Among Pregnant Women in Southwestern Nigeria

Hepatitis C virus (HCV) is the most common cause of cirrhosis, hepatocellular carcinoma and liver transplantation. While universal screening for other blood-borne viruses (BBVs) such as HIV and HBV among pregnant women is recommended in Nigeria, no such recommendation exist for HCV in the country. Despite recently developed direct-acting antiviral agents (DAAs) to cure HCV at high rates and at very high cost, the absence of an HCV vaccine or approved therapy during pregnancy makes prevention of vertical transmission impossible at the moment. Using a commercially available enzyme linked immunoassay technique, prevalence of antibodies to hepatitis C virus (anti-HCV) was determined among pregnant women attending antenatal clinics in Southwestern Nigeria. Of the 273 serum samples obtained from the pregnant women 9.5% was positive for anti-HCV antibody. There were differences in anti-HCV prevalence by age and locality. Results of the study confirm endemicity of HCV among pregnant women in the country, consequently, we advocate free screening, among other essential measures for HCV intervention in Nigeria. Keywords: HCV, Hepatitis, Pregnant women, Seroprevalence, ELISA, Nigeri

Women’s Behavioral Patterns in Domestic Tasks in Western Nigeria: Hazards Forecasting with Neural Network Classifier

Behavioral pattern is the characteristic ways a person acts and has been recognized as a cause of many home accidents (h-accd). This study reviewed the types and prevalence of injuries among women in domestic works and proposes a model using Artificial Neural Network (ANN) function to forecast the safety level of women in domestic duty. The study was conducted in some parts of Western Nigeria among 340 subjects (171 married and 169 unmarried) using questionnaire. SPSS was used for data analysis. The ANN function was developed in MATLAB 2015a using the subjects’ behavioral patterns and the model was used to predict safety in domestic duties (d-duties) among some women. ‘Cuts/laceration’ (40%) and ‘skin contact with hot substance’ (35.6%) were commonly reported. Carelessness (26.5%) and distraction (22.1%) were the main leading factors across the groups. Marital status and h-accd (Chi-square =4.323 and p= .038); ‘hours spent on domestic works’ and ‘the h-accd’ were both significant among other tested groups variables. With the developed ANN function, the results of the MSE was 0.33626 indicating that the function predicted the exact value. The result of the predicted h-accd (safety= -0.5445, hazards= 1.0228) in d-duties of the tested variables with the ANN function, showed a very low level of safety. The article concludes that the developed model is reliable and a recommended ergonomic tool useful in all homes, most especially where women perform most domestic works

Detection of Q129H Immune Escape Mutation in Apparently Healthy Hepatitis B Virus Carriers in Southwestern Nigeria

As the global effort to eradicate hepatitis B continues, immune escape mutations (IEMs) and drug resistance mutations (DRMs) affecting its diagnosis, treatment, and prevention are compromising this goal. However, knowledge about the prevalence and circulation of these mutations in Nigeria is scarce. Serum samples (n = 199) from apparently healthy prospective blood donors, pregnant women, and individuals presenting with fever in southwestern Nigeria were analyzed for the presence of IEMs and DRMs by means of nested PCR in the HBV S (HBs) and HBV polymerase (Pol) genes, followed by phylogenetic and mutational analyses. In total, 25.1% (n = 50/199) of samples were positive for HBV, as measured by PCR. In 41 samples (20.6%), both fragments could be amplified, whereas the HBs gene and the Pol gene fragment alone were detected in 0.5% (n = 1/199) and 4% (n = 8/199) of samples, respectively. Sequences were successfully obtained for all 42 HBs gene fragments but for only 31/49 Pol gene fragments (totaling 73 sequences from 44 individuals). All sequences were identified as HBV genotype E. IEMs were present in 18.2% (n = 8/44) of the sequences of HBV-positive individuals with available sequences. IEM Q129H was detected in eight out of the 44 (18.2%) HBV isolates sequenced in this study; however, no DRMs were observed. This study confirms the circulation of HBV IEMs and reports the presence of Q129H IEM for the first time in Nigeria. Intensified research on the dynamics of IEM is necessary in order to enhance the elimination of HBV.Peer Reviewe

Detection of Q129H Immune Escape Mutation in Apparently Healthy Hepatitis B Virus Carriers in Southwestern Nigeria

As the global effort to eradicate hepatitis B continues, immune escape mutations (IEMs) and drug resistance mutations (DRMs) affecting its diagnosis, treatment, and prevention are compromising this goal. However, knowledge about the prevalence and circulation of these mutations in Nigeria is scarce. Serum samples (n = 199) from apparently healthy prospective blood donors, pregnant women, and individuals presenting with fever in southwestern Nigeria were analyzed for the presence of IEMs and DRMs by means of nested PCR in the HBV S (HBs) and HBV polymerase (Pol) genes, followed by phylogenetic and mutational analyses. In total, 25.1% (n = 50/199) of samples were positive for HBV, as measured by PCR. In 41 samples (20.6%), both fragments could be amplified, whereas the HBs gene and the Pol gene fragment alone were detected in 0.5% (n = 1/199) and 4% (n = 8/199) of samples, respectively. Sequences were successfully obtained for all 42 HBs gene fragments but for only 31/49 Pol gene fragments (totaling 73 sequences from 44 individuals). All sequences were identified as HBV genotype E. IEMs were present in 18.2% (n = 8/44) of the sequences of HBV-positive individuals with available sequences. IEM Q129H was detected in eight out of the 44 (18.2%) HBV isolates sequenced in this study; however, no DRMs were observed. This study confirms the circulation of HBV IEMs and reports the presence of Q129H IEM for the first time in Nigeria. Intensified research on the dynamics of IEM is necessary in order to enhance the elimination of HBV

Women's behavioral patterns in domestic tasks in Western Nigeria : hazards forecasting with neural network classifier

Behavioral pattern is the characteristic ways a person acts and has been recognized as a cause of many home accidents (h-accd). This study reviewed the types and prevalence of injuries among women in domestic works and proposes a model using Artificial Neural Network (ANN) function to forecast the safety level of women in domestic duty. The study was conducted in some parts of Western Nigeria among 340 subjects (171 married and 169 unmarried) using questionnaire. SPSS was used for data analysis. The ANN function was developed in MATLAB 2015a using the subjects’ behavioral patterns and the model was used to predict safety in domestic duties (d-duties) among some women. ‘Cuts/laceration’ (40%) and ‘skin contact with hot substance’ (35.6%) were commonly reported. Carelessness (26.5%) and distraction (22.1%) were the main leading factors across the groups. Marital status and h-accd (Chi-square =4.323 and p= .038); ‘hours spent on domestic works’ and ‘the h-accd’ were both significant among other tested groups variables. With the developed ANN function, the results of the MSE was 0.33626 indicating that the function predicted the exact value. The result of the predicted h-accd (safety= -0.5445, hazards= 1.0228) in d-duties of the tested variables with the ANN function, showed a very low level of safety. The article concludes that the developed model is reliable and a recommended ergonomic tool useful in all homes, most especially where women perform most domestic works.http://vc.bridgew.edu/jiwshj2021Electrical, Electronic and Computer Engineerin