The cellular triumvirate: fibroblasts entangled in the crosstalk between cancer cells and immune cells

This review article will focus on subpopulations of fibroblasts that get reprogrammed by tumor cells into cancer-associated fibroblasts. Throughout this article, we will discuss the intricate interactions between fibroblasts, immune cells, and tumor cells. Unravelling complex intercellular crosstalk will pave the way for new insights into cellular mechanisms underlying the reprogramming of the local tumor immune microenvironment and propose novel immunotherapy strategies that might have potential in harnessing and modulating immune system responses

Investigating Effects of Site-Directed Mutagenesis on Activity and Expression of Endolysin in E.Coli BL21

  Endolysins, also referred to as "enzybiotics," are thought to be a promising class of antibiotics generated from enzymes. Their main advantage is a high degree of specificity over traditional broad-spectrum antibiotics. Endolysins don't harm the good microbiota and have specialized bactericidal functions that could be modified by molecular engineering. Moreover, endolysins have additional benefits, including quick bacterial cell lysis, minimal risk of resistance, synergistic activity with various antibiotics, and the capacity to work well in biofilms and on mucosal surfaces. Therefore, investigating and narrowing down the mutations that can increase the interaction of endolysin with substrates of the bacterial cell wall, it can be used as a therapeutic tool to combat bacterial resistance. For this reason, this project aims to utilize computational analysis of enzyme-substrate interactions to design point mutations using the site-directed mutagenesis GENEART System, which will increase the protein's lytic activity.</p