2 research outputs found

    Biological Resolution of Virulence Genes of Salmonella Species from different Microbiomes

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    The pathogenic promiscuity of virulence associated macromolecules in Salmonella infection is a key driver to their wide epidemiology and curtailing  such distribution is contingent upon proper clarification of these virulence genes. This study was therefore aimed at determining the virulence  genes of Salmonella species from different microbiomes. To achieve this, a total of three hundred (300) biological specimens were aseptically  collected and processed for Salmonella presence using the BAM USFDA technique prior to their genotypic characterization while virulence gene  detection was carried out in a primer specific polymerase chain reaction. Results obtained depict the distribution of the following Salmonella species  viz; Salmonella gallinarum 19(26.39%), Salmonella heidelberg 19(26.39%), Salmonella enteritidis 18(25%) and Salmonella typhimurium  16(22.22%) while the occurrence of the virulence genes (InvA, SopE, AgfA and SpvC) were Salmonella enteritidis ( 7(38.8), 6(33.3), 9(50), 3(16.7),  Salmonella typhimurium ( 5(26.3), 3(15.8), 2(10.5), 7(36.8)), Salmonella heidelberg (0(0), 8(50), 4(25), 4(25), and Salmonella gallinarum (12(63.2),  6(31.6), 2(10.5), 7(36.8)) respectively. It was however found that the different microbiomes analyzed were ubiquitously rich in virulence genes  associated Salmonella species.   La promiscuitĂ© pathogène des macromolĂ©cules associĂ©es Ă  la virulence dans l’infection Ă  Salmonella est un facteur clĂ© de leur large Ă©pidĂ©miologie  et la rĂ©duction de cette distribution dĂ©pend de la clarification appropriĂ©e de ces gènes de virulence. Cette Ă©tude visait donc Ă  dĂ©terminer les gènes  de virulence des espèces de Salmonella de diffĂ©rents microbiomes. Pour ce faire, un total de trois cents (300) Ă©chantillons biologiques ont Ă©tĂ©  collectĂ©s et traitĂ©s de manière aseptique pour la prĂ©sence de Salmonella Ă  l’aide de la technique BAM USFDA avant leur caractĂ©risation gĂ©notypique  tandis que la dĂ©tection du gène de virulence a Ă©tĂ© effectuĂ©e dans une rĂ©action en chaĂ®ne par polymĂ©rase spĂ©cifique Ă  l’amorce. Les rĂ©sultats  obtenus dĂ©crivent la distribution des espèces de Salmonella suivantes, Ă  savoir ; Salmonella gallinarum 19(26,39%), Salmonella heidelberg  19(26,39%), Salmonella enteritidis 18(25%) et Salmonella typhimurium 16(22,22%) alors que la prĂ©sence des gènes de virulence (InvA, SopE, AgfA et  SpvC) Ă©tait Salmonella enteritidis ( 7(38,8), 6(33,3), 9(50), 3(16,7), Salmonella typhimurium ( 5(26,3), 3(15,8), 2(10,5), 7(36,8)), Salmonella heidelberg (0(  0), 8(50), 4(25), 4(25) et Salmonella gallinarum (12(63.2), 6(31.6), 2(10.5), 7(36.8)) respectivement. diffĂ©rents microbiomes analysĂ©s Ă©taient  ubiquitairement riches en gènes de virulence associĂ©s aux espèces de Salmonella  &nbsp

    Impact of depth of clinical response on outcomes of acute myeloid leukemia patients in first complete remission who undergo allogeneic hematopoietic cell transplantation

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    Acute myeloid leukemia (AML) patients often undergo allogeneic hematopoietic cell transplantation (alloHCT) in first complete remission (CR). We examined the effect of depth of clinical response, including incomplete count recovery (CRi) and/or measurable residual disease (MRD), in patients from the Center for International Blood and Marrow Transplantation Research (CIBMTR) registry. We identified 2492 adult patients (1799 CR and 693 CRi) who underwent alloHCT between January 1, 2007 and December 31, 2015. The primary outcome was overall survival (OS). Multivariable analysis was performed to adjust for patient-, disease-, and transplant-related factors. Baseline characteristics were similar. Patients in CRi compared to those in CR had an increased likelihood of death (HR: 1.27; 95% confidence interval: 1.13-1.43). Compared to CR, CRi was significantly associated with increased non-relapse mortality (NRM), shorter disease-free survival (DFS), and a trend toward increased relapse. Detectable MRD was associated with shorter OS, shorter DFS, higher NRM, and increased relapse compared to absence of MRD. The deleterious effects of CRi and MRD were independent. In this large CIBMTR cohort, survival outcomes differ among AML patients based on depth of CR and presence of MRD at the time of alloHCT. Further studies should focus on optimizing post-alloHCT outcomes for patients with responses less than CR
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