Improved Colorimetric Determination of Reserpine in Tablets Using 4-Caboxyl-2,6-dintrobenzene diazonium ion (CDNBD)

Purpose: To develop a simple, rapid and improved colorimetric method for the assay of reserpine in tablets Method: The method is based on the aromatic ring coupling of reserpine with 4-carboxyl-2,6-dinitrobenzene diazonium ion with the consequent formation of an azo adduct. Optimization of reaction conditions and validation were carried out and the method applied to assay of reserpine in tablets. Result: Reserpine coupled readily with CDNBD and optimization of experimental conditions showed the reaction to be completed in 10 min at room temperature. A 1:1 drug to reagent stoichiometric ratio was obtained for the azo adduct formed. The adduct exhibited a bathochromic shift with respect to the drug and pronounced hyperchromic shift with respect to the reagent. Sample analyses were done using a colorimeter at 470 nm. The assays were linear and reproducible over the concentration range of 2.25 -24 µg/mL. The new method was successfully applied in the assay of reserpine in tablets with a performance similar to the official (USP) spectrophotometric method (p > 0.05). This method represents a profound improvement on the previously reported colorimetric method for reserpine. Conclusion: The method developed is rapid and could find application in in-process quality control of reserpine. Keywords: Reserpine, colorimetry, 4-Caboxyl-2,6-dintrobenzene diazonium ion (CDNBD), diazo coupling > Tropical Journal of Pharmaceutical Research Vol. 6 (2) 2007: pp. 695-70

Iris feature extraction: a survey

Biometric as a technology has been proved to be a reliable means of enforcing constraint in a security sensitiveenvironment. Among the biometric technologies, iris recognition system is highly accurate and reliable becauseof their stable characteristics throughout lifetime. Iris recognition is one of the biometric identification thatemploys pattern recognition technology with the use of high resolution camera. Iris recognition consist of manysections among which feature extraction is an important stage. Extraction of iris features is very important andmust be successfully carried out before iris signature is stored as a template. This paper gives a comprehensivereview of different fundamental iris feature extraction methods, and some other methods available in literatures.It also gives a summarised form of performance accuracy of available algorithms. This establishes a platform onwhich future research on iris feature extraction algorithm(s) as a component of iris recognition system can bebased.Keywords: biometric authentication, false acceptance rate (FAR), false rejection rate (FRR), feature extraction,iris recognition system

Contraceptive uptake among women attending family planning clinic in a Nigerian tertiary health facility: a 6 year review

Background: Contraceptive uptake is one of the most important determinants of pregnancy rates and birth rates in the world. Aim: To determine the principal trends in contraceptive use from 2004-2009 in the family planning unit of a tertiary health centre in South-Western Nigeria, and to identify the effect of age, marital status and parity on the choice of contraceptive method.Methods: The record of 1,862 clients attending the Family Planning unit of Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Osun-State, Nigeria for the period between 2004-2009 were reviewed. Information related to the age, marital status, parity and the choice of contraception were obtained using a structured proforma and the data analyzed with SPSS version 16. Pearson chi-square test was used as test of significance where applicable.Results: Within the study period, the contraceptive uptake was 13.2%. Copper-T IUCD was the most commonly used method of contraception (77.9%), followed by the progestogen only injectable contraceptives (12.6%), then oral pills (4.1%) and progestin implants (2.3%). Single women and women of low parity are more likely to use pills than IUCD (69% vs. 38.3%) while long acting reversible contraception (injectables and IUCD) are preferred by women with higher parity (P=0.000).Conclusions: IUCD is the most popular method of contraception in Ife-Ijesha area of Nigeria. Contraceptive uptake is relatively low among the women. Age and parity are key influences on the uptake and choice of contraception practiced by the women, while the influence of marital status is not statistically significant

MEAT QUALITY CHARACTERISTICS OF IMPROVED INDIGENOUS CHICKENS (FUNAAB-ALPHA) FED TURMERIC (Curcuma longa) OR CLOVE (Syzygium aromaticum) AS FEED ADDITIVES

The experiment was conducted to investigate meat quality of improved indigenous chickens (FUNAAB alpha) fed diet containing turmeric (Curcuma longa) and clove (Syzigium aromaticum) powders. One hundred and thirty-five (135) mixed sexed day-old improved indigenous FUNAAB alpha chicks were brooded collectively for two weeks, and assigned into three (3) treatment groups. Each treatment was replicated three times, consisting of fifteen (15) birds. Feed and water were provided ad-libitum through the duration of experiment. The control group was fed the basal diet at starter and  finisher phases (T1), while the other groups were fed clove (Syzygium aromaticum) powder at 3 g/kg (T2) and turmeric (Curcuma longa) (T3) powder at 4 g/kg respectively as additives.   Data obtained were subjected to a One – Way Analysis of Variance in a Completely Randomised Design. Muscle obtained after evisceration was subjected to quality analysis. Proximate composition of meat obtained from chickens fed the basal diet had the highest (p<0.05) moisture content value of 75.45% compared to other treatments. Water absorptive capacity and cook loss of meat obtained from chickens fed 4 g/kg dietary turmeric showed (p<0.05) values of 3% and 16.4% respectively. Meat sensory properties analysed revealed (p<0.05) score in groups fed 3g/kg and 4 g/kg clove and turmeric powders respectively, for tenderness (6.14) and overall acceptability (7.19). Meanwhile (p<0.05) lipoprotein profile was observed in group fed 4 g/kg turmeric powder. Inclusion, turmeric and clove had beneficial effects on FUNAAB Alpha. &nbsp

REPRODUCTIVE RESPONSE TO INCLUSION OF GRADED LEVELS OF Ipomoea purpurea LEAF MEAL (MORNING GLORY) IN DIETS OF LAYING CHICKENS

Reproductive responses of laying birds fed Ipomea purpurea leaf meal (IPML) were investigated. Atotal of 40, 25-weeks old birds grouped into four treatments of 0, 2.5, 5.0 and 7.5% inclusion levels of IPML were used for this experiment in a completely randomized design layout. The birds were artificially inseminated with semen from cockerels on controlled diet thrice in a week. A total of 400 eggs were collected and set in an incubator. There were no significant differences (p>0.05) in Hen day production,feed/dozen eggs and the egg characteristics investigated. The blood glucose levels significantly decreased (p<0.05) while level of progesterone significantly increased (p<0.05) with increase inlevel of IPLM inclusion. The percentages of fertility and hatchability were high while the survivability was 100%. It can be concluded that IPLM inclusion level of up to 7.50% supports high fertility, hatchability and survivability and could be used in breeder diets for the production of fertile eggs

COMPARATIVE ANALYSIS OF TWO BIOMETRIC ACCESS CONTROL SYSTEMS

This paper compared two Biometric Access Control Systems (BACS). The BACSs employed iris pattern for their authentication. Fast Fourier Transform-driven Access Control System (FACS) uses global iris features while Haar Wavelet Transform-driven Access Control System (HACS) uses local iris features for its template generation. Principal Component Analysis (PCA) was employed to select principal components of the extracted features (local and global). Fuzzy clustering was used for classification and Euclidean Distance (ED) as the distance metric. Experimental result showed that it took more time to train the HACS than FACS because of its intrinsic location in the iris images. It was discovered that global features driven Access Control System (FACS) with EER being 7.78 outperformed the local features driven Access Control System (HACS) with EER of 8.05. Though the two systems satisfied the benchmark of 80% for Recognition Accuracy (RA) of Biometric Systems, FACS exhibited RA of 89.87% while HACS achieved a RA of 83.83% when tested on iris images captured with CMITECH DMX-10 Portable USB 5.0 M pixel CCD Iris Camera at automatic convenient eye distances. Performance of global and local features on other biometric recognition systems can be tested and a means of combining the two features for hybridization can also be sought

Petrology, Physicochemical and Thermal Analyses of Selected Cretaceous Coals from the Benue Trough Basin in Nigeria

Abundant coal resources that were previously neglected due to a crude oil boom need revitalisation and integration into the national electricity mix to address the energy demands of the Nigerian population. Selected coal samples from the Benue Trough sedimentary basin in Nigeria were examined by various techniques, including proximate and ultimate analyses, organic petrography, Fourier transform infrared ray spectroscopy, and thermogravimetric analysis. Based on vitrinite reflectance, the Lafia-Obi (OLB), Garin Maiganga (GMG), Imiegba (IMG), and Okaba (OKB) coals are classified as subbituminous, while the Lamja1 (LMJ1), Lamja2 (LMJ2) and Chikila (CHK) coals are high volatile B bituminous. The Enugu (ENG) coal is on the boundary between subbituminous and high volatile C bituminous. Organic petrographic results indicate vitrinite and fusinite contents steadily increase from the Lower Benue Trough coals to the Upper Benue Trough coals, while semifusinite and total mineral contents follow a reverse pattern. Thermal decomposition occurred in three stages, i.e., drying, devolatilization, and coke formation above 700 °C; and the coal reactivity follows the following order, ENG \u3e IMG \u3e IGH \u3e CHK \u3e LMJ \u3e OKB \u3e GMG \u3e LFB. The higher temperatures (above 900 °C) are required to decompose the coals for efficient energy recovery. The LMJ1, LMJ2, OLB, CHK, GMG, and OKB coals can be exploited for electricity power generation. However, the Imeagha and Enugu coals are best suitable for both cement and power generation

Gold Nanoparticle-Quantum Dot Fluorescent Nanohybrid:Application for Localized Surface Plasmon Resonance-induced Molecular Beacon Ultrasensitive DNA Detection

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Novi pristup spektrofotometrijskom određivanju metronidazola i tinidazola koristeći p-dimetilaminobenzaldehid

A new approach to the spectrophotometric determination of metronidazole (MZ) and tinidazole (TZ) has been developed. The procedure involves coupling of diazotized nitroimidazoles with p-dimethylaminobenzaldehyde (DMAB) to form a greenish-yellow solution. Optimal temperature and time for diazotization were 0 oC (iced) and 3 minutes and 30 oC and 2 minutes for coupling was, respectively, for both MZ and TZ. Coloured adducts of MZ and TZ showed peaks at 406 nm and 404 nm, respectively, which were selected as analytical wavelengths. The reaction with p-DMAB occurred in a 1:1 mole ratio. Beer’s law was obeyed within the 4.8–76.8 µg mL1 concentration range with low limits of detection. The azo adducts were stable for over a week. Molar absorptivities were 1.10 x 103 (MZ) and 1.30 x 103 L mol1 cm1 (TZ). Overall recoveries of MZ and TZ from quality control samples were 103.2 ± 1.3 and 101.9 ± 1.3 % over three days. There was no interference from commonly utilized tablet excipients. No significant difference was obtained between the results of the new method and the BP titrimetric procedures. The azo approach using the p-dimethylaminobenzaldehyde procedure described in this paper is simple, fast, accurate and precise. It is the first application of DMAB as a coupling component in the diazo coupling reaction.U radu je opisan novi način spektrofotometrijskog određivanja metronidazola (MZ) i tinidazola (TZ). Postupak uključuje reakciju diazotiranog nitroimidazola s p-dimetilaminobenzaldehidom (DMAB), pri čemu nastaje zelenkasto-žuta otopina. Optimalna temperatura i vrijeme za diazotaciju su 0 oC (ledena kupelj) i 3 minute, a za reakciju kondenzacije 30 oC i 2 minute. Obojeni adukti imaju maksimum apsorpcije pri 406, odnosno 404 nm pa su te valne duljine izabrane za analitički postupak. Reakcija s p-DMAB zbiva se u množinskom omjeru 1:1. Reakcija slijedi Beerov zakon u koncentracijskom rasponu 4,8–76,8 µg mL1 s niskim granicama detekcije. Azo adukti su stabilni preko tjedan dana. Molarna apsorptivnost bila je 1,10 × 103 (MZ), odnosno 1,30 × 103 L mol1 cm1 (TZ). Ukupni povrat MZ i TZ iz kontrolnih uzoraka bio je 103,2 ± 1,3, odnosno 101,9 ± 1,3 % tijekom tri dana. Nije zamijećena nikakva interferencija uobičajenih pomoćnih tvari koje se koriste za tabletiranje. Ne postoji značajna razlika između rezultata dobivenih novom metodom i rezultata dobivenih BP titrimetrijskim postupkom. Metoda određivanja opisana u ovom radu je jednostavna, brza, pogodna, točna i precizna i po prvi puta uključuje DMAB u reakciji diazo kopulacije