Comparative Antioxidants Status of Leaves Extracts of Some Common Antimalarial Plants in West Africa

Objective: Malaria chemotherapy remains relevance and gives way to the re-evaluation of medicinal plants that has already gain approval in the traditional treatments of Malaria. This work evaluated and compared the antioxidant status of methanol leaves extracts of Azadirachta Indica (MAI), Vernonia Amygdalina (MVA) and Carica Papaya (MCP). Methods: Phenols, Flavonoids,percentage inhibition of lipid peroxidation and radical scavenging activities using 1,1-diphenyl-2- picryl hydrazyl (DPPH) and Hydroxyl radicals were determined spectrophotometrically based on international standardized methods . Results: Total Phenolic content in garlic acid equivalence (GAE)were expressed maximally at 700μg/ml by 0.015±0.002, 0.019±0.017, 0.013±0.006 mg/g and flavonoids contents at 350μg/ml by 0.063±0.004, 0.020±0.031, 0.049±0.002 Mg/g quercetin equivalence (QE) for MAI, MVA and MCP respectively. DPPH scavenging activities of 78.60, 55.55 and 54.96 % were obtained at 350μg/ml by MAI, MVA and MCP respectively in the order MAI>MVA>MCP. At 300μg/ml, the extracts scavenged hydroxyl radicals significantly( p≤0.05) by72.00, 77.80 and 53.15 % in the order MVA>MAI>MCP. Intriguingly, extracts also convered significantly (p≤0.05), 50% cell protection as they inhibited lipid peroxidation by50.00, 66.20 and 64.30% in the order MVA>MCP>MAI at 350μg/ml, respectively. Conclusion: Antioxidative properties exhibited by extracts may be correlated with their antimalarial functions, bioactive contents and suggestive of MVA as more potent antimalarial of the evaluated plants which may serve as template for malaria drugs and its local usage encouraged in poverty- stricken malarial-endemic areas of West Africa. Keywords: AzadirachtaIndica, Antimalarial, Antioxidants, CaricaPapaya, Malaria, Vernonia Amygdalina

Comparative Antioxidants Status of Leaves Extracts of Some Common Antimalarial Plants in West Africa

Objective: Malaria chemotherapy remains relevance and gives way to the re-evaluation of medicinal plants that has already gain approval in the traditional treatments of Malaria. This work evaluated and compared the antioxidant status of methanol leaves extracts of Azadirachta Indica (MAI), Vernonia Amygdalina (MVA) and Carica Papaya (MCP). Methods: Phenols, Flavonoids,percentage inhibition of lipid peroxidation and radical scavenging activities using 1,1-diphenyl-2- picryl hydrazyl (DPPH) and Hydroxyl radicals were determined spectrophotometrically based on international standardized methods . Results: Total Phenolic content in garlic acid equivalence (GAE)were expressed maximally at 700μg/ml by 0.015±0.002, 0.019±0.017, 0.013±0.006 mg/g and flavonoids contents at 350μg/ml by 0.063±0.004, 0.020±0.031, 0.049±0.002 Mg/g quercetin equivalence (QE) for MAI, MVA and MCP respectively. DPPH scavenging activities of 78.60, 55.55 and 54.96 % were obtained at 350μg/ml by MAI, MVA and MCP respectively in the order MAI>MVA>MCP. At 300μg/ml, the extracts scavenged hydroxyl radicals significantly( p≤0.05) by72.00, 77.80 and 53.15 % in the order MVA>MAI>MCP. Intriguingly, extracts also convered significantly (p≤0.05), 50% cell protection as they inhibited lipid peroxidation by50.00, 66.20 and 64.30% in the order MVA>MCP>MAI at 350μg/ml, respectively. Conclusion: Antioxidative properties exhibited by extracts may be correlated with their antimalarial functions, bioactive contents and suggestive of MVA as more potent antimalarial of the evaluated plants which may serve as template for malaria drugs and its local usage encouraged in poverty- stricken malarial-endemic areas of West Africa

Antimicrobial Stewardship Implementation in Nigerian Hospitals: Gaps and Challenges

Background: Antimicrobial resistance (AMR) is a major clinical challenge globally. It is mainly a consequence of inappropriate prescribing and use of antibiotics. Antimicrobial stewardship (AMS) ensures that antibiotics are prescribed and used appropriately. This study assessed AMS practice in selected Nigerian hospitals.Methodology: This was a cross sectional survey of 20 Federal, State and Private tertiary hospitals randomly selected from the six geopolitical zones of Nigeria. Using an adapted WHO tool on AMS, data were collected from each hospital as regard the existence of AMS committee, Accountability and Responsibility, AMS actions, Education and Training, Monitoring and Evaluation, Infection Prevention and Control (IPC) practice, facilities to support AMS, and challenges to AMS implementation. Gaps and challenges to the implementation of the AMS among the hospitals were identified.Results: Only 6 (30%) of the 20 hospitals had AMS committees while 2 (10%) had any evidence of leadership commitment to AMS. All the hospitals had laboratory facilities to support culture and sensitivity testing. There were no regular AMS-related education or training, monitoring, evaluation or reporting activities in the hospitals, except in 7 (25%) that had participated in the global point prevalence survey (Global-PPS) of antimicrobial use and resistance being hosted by the University of Antwerp, Belgium. Challenges impeding AMS activities included lack of human and financial resources, prescribers’ opposition, lack of awareness and absence of AMS committees. Most of the gaps and challenges bordered on seeming lack of knowledge and inadequate communication among prescribers and other stakeholders.Conclusion: There is need for intense education and training activities for prescribers and other stakeholders, including but not limited to hospital administrators. Keywords: Survey, Antimicrobial Stewardship, Antimicrobial Resistance; Nigeria   French title: Mise en œuvre de la gestion des antimicrobiens dans les hôpitaux Nigérians: lacunes et défis Contexte: La résistance aux antimicrobiens (RAM) est un défi clinique majeur à l'échelle mondiale. C'estprincipalement une conséquence d'une prescription et d'une utilisation inappropriées d'antibiotiques. La gestion des antimicrobiens (AMS) garantit que les antibiotiques sont prescrits et utilisés de manière appropriée. Cette étude a évalué la pratique de l'AMS dans certains hôpitaux Nigérians. Méthodologie: Il s'agissait d'une enquête transversale de 20 hôpitaux tertiaires fédéraux, d'État et privéssélectionnés au hasard dans les six zones géopolitiques du Nigéria. À l'aide d'un outil OMS adapté sur l'AMS, des données ont été collectées auprès de chaque hôpital en ce qui concerne l'existence d'un comité AMS, la responsabilité et la responsabilité, les actions AMS, l'éducation et la formation, le suivi et l'évaluation, la  pratique de prévention et de contrôle des infections (IPC), les installations pour soutenir l'AMS. et les défis de la mise en œuvre de l'AMS. Les lacunes et les défis liés à la mise en œuvre de l'AMS parmi les hôpitaux ont été identifiés. Résultats: Seuls 6 (30%) des 20 hôpitaux avaient des comités AMS tandis que 2 (10%) avaient des preuves d'engagement du leadership envers l'AMS. Tous les hôpitaux disposaient d'installations de laboratoire pour soutenir la culture et les tests de sensibilité. Il n'y avait pas d'activités régulières d'éducation ou de formation, de suivi, d'évaluation ou de rapportage liées à la MGS dans les hôpitaux, sauf dans 7 (25%) qui avaient participé à l'enquête mondiale sur la prévalence ponctuelle (Global-PPS) de l'utilisation et de la résistance aux  antimicrobiens organisée par l'Université d'Anvers, Belgique. Les défis entravant les activités de l'AMS  comprenaient le manque de ressources humaines et financières, l'opposition des prescripteurs, le manque de sensibilisation et l'absence de comités AMS. La plupart des lacunes et des défis se limitaient à un manque apparent de connaissances et à une communication inadéquate entre les prescripteurs et les autres intervenants.Conclusion: Des activités d'éducation et de formation intensives sont nécessaires pour les prescripteurs et autres intervenants, y compris, mais sans s'y limiter, les administrateurs d'hôpitaux. Mots clés: enquête, gestion des antimicrobiens, résistance aux antimicrobiens; Nigeria   &nbsp

Corrigendum: Antimicrobial Stewardship Implementation in Nigerian Hospitals: Gaps and Challenges

No Abstract

Emergence and spread of two SARS-CoV-2 variants of interest in Nigeria.

Identifying the dissemination patterns and impacts of a virus of economic or health importance during a pandemic is crucial, as it informs the public on policies for containment in order to reduce the spread of the virus. In this study, we integrated genomic and travel data to investigate the emergence and spread of the SARS-CoV-2 B.1.1.318 and B.1.525 (Eta) variants of interest in Nigeria and the wider Africa region. By integrating travel data and phylogeographic reconstructions, we find that these two variants that arose during the second wave in Nigeria emerged from within Africa, with the B.1.525 from Nigeria, and then spread to other parts of the world. Data from this study show how regional connectivity of Nigeria drove the spread of these variants of interest to surrounding countries and those connected by air-traffic. Our findings demonstrate the power of genomic analysis when combined with mobility and epidemiological data to identify the drivers of transmission, as bidirectional transmission within and between African nations are grossly underestimated as seen in our import risk index estimates

n-Alkane Profile of Underground Water near a Dump-site at Balogun-biiro in Okebaale, Oshogbo Osun State

Well-water samples were collected from the vicinity of Balogun–biiro dump-site located in Okebaale Oshogbo, Osun state, Nigeria. The aim of this research work is to provide background information on the concentration of n-alkanes in the well-water in order to assess its pollution status and probable sources. The n-alkane was determined qualitatively and quantitatively using GC-FID. The concentration of n-alkanes in the water samples was, 8.07, 9.64, 9.75, 8.43, 10.4, and 10.1 mg/L in S1, S2, S3, S4, S5, and S6 respectively. The highest concentration of n-alkanes was recorded in the water samples collected from a well located in a residential building very close to the dumpsite as well as the mechanic workshop (i.e. S5) while the lowest concentration was recorded in S1 (well-water relatively far from the dump-site). Various n-alkanes diagnostic ratios obtained indicated anthropogenic sources (i.e. both petrogenic and pyrogenic) and showed moderate level of pollution in the area under study. Keywords: GC-FID; aliphatic hydrocarbon; pollution; anthropogenic; mechanic worksho

Int J Infect Dis

Lassa fever (LF) is an endemic viral hemorrhagic fever in West Africa. Among the serious complications of the disease are neurological manifestations whose spectrum is incompletely known. Here we report the case of a 61-year-old man who developed a delayed-onset paraparesis a few weeks after getting infected with Lassa virus thereby suggesting a possible association between LF and spinal cord disorders