Antimicrobial susceptibility of urinary bacterial isolates of pregnant women attending antenatal clinics of selected hospitals in Ilorin, Nigeria

Background: Urinary tract infection (UTI) in pregnancy is associated with significant morbidity for both mother and baby. This study was aimed at determining the urinary bacterial isolates and their susceptibility to selected antibiotics among pregnant women attending antenatal clinics of selected Hospitals in Ilorin, Nigeria. Methodology: A total of 300 pregnant women between the ages of 15 and 44 years were selected by random sampling technique from antenatal clinics of three randomly selected healthcare facilities in Ilorin, Nigeria; Civil Service Hospital, Sobi Specialist Hospital and Okelele Primary Health Center, between July and October 2021. Clean-catch mid-stream specimens of voided urine were collected from each participant, cultured on Cysteine- Lactose-Electrolyte Deficient (CLED) and Blood agar plates, and incubated aerobically at 37oC for 24 hours. The presence of significant bacteriuria (≥105 CFU/ml) was determined on the culture plate using the plate count method. Antibiotic susceptibility testing to selected antibiotics was done using Kirby-Bauer disk diffusion technique. Cefoxitin (30g) was used as surrogate to determine phenotypic methicillin resistance in staphylococcus isolates, and the methicillin resistance (mecA) gene was detected by conventional PCR assay. Results: Of the 300 pregnant participants, 49 (16.3%) were symptomatic for UTI while 251 (83.7%) were asymptomatic. Significant bacteria (monomicrobial) were isolated in 44 (14.7%) pregnant women; 28 (11.2%) of 251 asymptomatic and 16 (32.6%) of 49 symptomatic women, which showed that significant bacteriuria rate was higher with symptomatic than asymptomatic women (OR=3.861, 95% CI=1.889–7.893, p=0.0005), but significant bacteriuria rate did not differ with respect to age group of the women (x2=1.463, p=0.4811). The most common bacterial isolates were Escherichia coli (38.6%, 17/44) followed by coagulase negative staphylococci (CoNS) (22.5%, 9/44), Klebsiella pneumoniae (18.2%, 8/44), Staphylococcus aureus (15.9%, 7/44), Enterococcus sp (4.6%, 2/44) and Pseudomonas sp (2.3%, 1/44). Gram negative isolates showed high resistance rate of 73.1% to ampicillin and 65.4% to amoxicillin-clavulanic acid while Gram-positive isolates showed high resistant rate of 94.1% to penicillin. The Gram-positive isolates showed high susceptibility rate of 100% while the Gram-negative isolates showed moderate susceptibility of 69.2% to nitrofurantoin. Four of the 9 (44.4%) CoNS isolates were cefoxitin resistant and all the 4 (100%) carried mecA gene. Conclusion: The isolation of bacterial pathogens resistant to the commonly prescribed antibiotics from pregnant women symptomatic and asymptomatic for UTI calls for early screening of all pregnant women for UTI during antenatal care service delivery

Nutritional Value of some Edible Mushrooms from Egbe Farmland, West Yagba Local Government Area, Kogi State, Nigeria

Studies were carried out on four different Species of wild mushrooms: Lentinus subnudus, Chlorophyllum molybditis, Marasmus species and Pleurotus tuberregium. The fungal species were analyzed for their nutritional compositions, biological and Structural characteristics. The fungal species were rich in proteins (amino acids) and carbohydrates. The fibres contained in the different species were also good sources of roughages. Moreover, their low acidity confirms their edibility. This study strongly recommends the commercialization of mushroom production as a means of additional source of protein requirements and income to the people in the developing economy of Nigeri

Pectinolytic activity of aspergillus niger and Aspergillus flavus grown on grapefruit (citrus Parasidis) peel in solid state fermentation

The present study was aimed at studying pectinolytic activity of resident fungi isolated from decomposing grapefruit (Citrus parasidis) peels in solid state fermentation. Grape fruit peel was subjected to natural fermentation and the fermenting fungi were isolated, characterized and identified using standard microbiological methods. The isolated fungi were in turn used for fermentation to determine their pectinolytic activity through solid state fermentation technique. Culture parameters such as incubation period, temperature, moisture content and addition of salts supplements were optimized during the research for five days. The identified fungi were Aspergillus Niger and Aspergillus flavus. The peak of pectinolytic activity was at day three of fermentation when the highest pectinase activity of 13.32 μmol/mg/min was recorded for A. Niger and 11.32 μmol/mg/min for A. flavus. Optimum temperature and pH for pectinase activity by A. Niger and A. flavus was at 40 0C and pH 7.5 and 7.7 respectively. The use of salt supplemented substrate did not alter enzyme activity. In conclusion, the isolated fungi could be promising organisms for pectinolytic enzyme production on grape peel as substrate. Keywords: Grapefruit, Pectinolytic Activity, Fungi, Fermentation, Aspergillu

Characterization of methicillin-resistant Staphylococcus aureus isolates from apparently healthy individuals in Malete, Kwara state, Nigeria

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a common and continuously growing cause of nosocomial and community-acquired staphylococcal infections around the world. Screening for colonization with MRSA is a major aspect of control and limiting the spread of infections cause by this organism. We investigated the carriage of MRSA among apparently healthy individuals in four rural villages: Eleburu, Tapa, Atere and Apo all around semi-urban town-Malete, in Moro Local Government of Kwara State, Nigeria.Methods: Nasal swabs were collected from volunteered individuals and were cultured on mannitol salt agar and blood agar for isolation and identification of Staph aureus using standard microbiological techniques. Susceptibility to cefoxitin disc (30 ag) was used to determine MRSA status of the isolates. Molecular method was used to detect the gene responsible for resistance among MRSA isolates. Antimicrobial susceptibility test to commonly prescribed antibiotics was carried out using discs diffusion method.Results: Total number of individuals carrying Staph aureus in their nostrils was 42 (37.2 %). Antibiotics susceptibility profile of Staph aureus isolates showed 100 % resistance to cefuroxime, cefotaxime, cloxacillin and augmentin, and were 87 %, 81 %, 69 % and 23.8 % and 19 % resistant to tetracycline, ceftriaxone, erythromycin, ofloxacin and gentamicin respectively. A total of 6 (14%) Community –Acquired MRSA (CA-MRSA) isolates were recovered from individuals living in these villages. Molecular method detected muc and mecA genes in all the 6 (100%) CA-MRSA isolates and lukS-lukF was detected in 3 (50%) of the isolates.Conclusion: Detection of CA-MRSA strains among these rural dweller indicates that they are harbouring enhance virulence organism that may manifest a more severe disease condition. The danger associated with high prevalence of multidrug resistant Staph aureus and CAMRSA; and its consequential effects of poor drug administration in Nigeria was discussed. There is need to establish a more strict legislation and enforcement on drug control; and a body that would monitor production and appropriate use of antibiotics in the Nigeria.KEYWORDS: CA-MRSA, Staph aureus, Antibiotics, Rural Villages and Molecular Characterizatio

Itaconic Acid Production from Date Palm (Phoenix Dactylifera L) Using Fungi in Solid State Fermentation

This study evaluates the potentials of Phoenix dactylifera L (Date fruits) as possible alternative raw materials for itaconic acid production using naturally occurring fungi. Date fruit (pulp) was used as a substrate in solid state fermentation for the production of itaconic acid using naturally occurring fungus.The date fruit (pulp) was de-capped from its seed manually with the aid of a knife and dried in an oven at 60 0C and was grounded using an Excella Mixer grinder. The fungus used was naturally isolated by fermentation of substrate (date pulp) and was identified as Aspergillus niger. Proximate analysis was carried out on the substrate using standard methods. Parameters such as substrate concentration, inoculum size and fermentation period were varied using standard methods to determine its effect on itaconic acid production. Assay for itaconic acid production was carried out using standard methods at a wavelength of 385nm. Amount of itaconic acid produced was derived by translation of absorbance values on the itaconic acid curve. The substrate had a high carbohydrate content of 72.29%. The fermentation results showed maximum production of itaconic acid of 20.75±0.25mg/ml using 40g substrate, 15.13±1.13mg/ml using 2 ml inoculums size of spore suspension (2×105 spores/ml) and a maximum yield of 16.88±0.13mg/ml at day 1 of fermentation period. On optimization with 40g substrate and 2 ml inoculums for 3 days a maximum yield was observed at day 2 of fermentation with a maximum yield of 25.00±1.00mg/ml. The highest acidic level throughout the fermentation period was observed to be at pH 4.2. From the study it was concluded that date pulp is a promising substrate and could be utilized by Aspergillus niger for the production of itaconic acid

Determinants of Depression among University Students in Nigeria

الملخص: تبحث الدراسة محددات الاكتئاب بين طلاب الجامعات في نيجيريا، وقد اُستخدم المنهج الوصفي من خلال تطبيق استبيان في هذه الدراسة. شملت عيّنة الدراسة جميع طلاب الجامعات في نيجيريا، وتكونت عينة الدراسة من 1050 طالبًا جامعيًا. قام الباحثون بإعداد استبانة خاصة للدراسة، باستخدام نمط الاستجابات المغلقة. أظهرت النتائج أن العمر والدخل عوامل مهمة في مستوى الاكتئاب لدى طلاب الجامعات في نيجيريا، بينما نوع الأسرة لم يكن له تأثير، وعليه فقد جاءت التوصيات على أنه يجب على إدارة الجامعة تحسين خدمات الصحة العقلية من خلال زيادة التثقيف الصحي للكشف المبكر والسيطرة على حالات الاكتئاب بين الطلاب.Abstract: The study examined the determinants of depression among university students in Nigeria. Additionally, descriptive research design of survey type was used in this study. The population for this study comprised of all Nigeria undergraduate students. The sample for the study was 1,050 undergraduates. The instrument used for this study was a self-constructed, structured close ended questionnaire. Findings revealed that age and income were significant determinants of depression among university students in Nigeria while family type is not. It was therefore recommended that the University management should strengthen mental health services through health education to enable early detection and management of depression among students

Assessment of performance indices of selected gas turbine power plants in Nigeria

In this study, performance assessment of selected gas turbine power plants in Nigeria was evaluated using performance indices. The results of the study showed that for the period under review (2006–2010), the percentage shortfalls from the target energy in the selected power plants range from 26.33% to 86.61% as against the acceptable value of 5–10%. The capacity factor of the selected power plants varies from 16.88% to 73.67% as against the international value of 50–80%. The plant use factor varies from 45.89% to 97.03% and the utilization factor varies from 6.31% to 93.074% as against the international best practice of over 95%. From this result, it can be concluded that the generating units were underutilized. This is due to inadequate routine maintenance and equipment fault development. The analyses of reliability indicators revealed that the mean time between failures varies from 5.42 to 378.44 h, the mean time to repair varies from 18.3 to 153.88 h and the plant availability varies from 12.86% to 91.31% as against the Institute of Electrical and Electronics Engineers recommended standard of 99.9%. Evaluation of operating figures of the selected power plants revealed that starting reliability (SR) and operating reliability vary from 71.95% to 93.9% and 5.33% to 55%, respectively. The SR of the selected power plants is low in value compared with standard value of 99.9%. The statistical analysis carried out on plant availability revealed that at 95% confidence level; there is a significant difference in availability of the selected power plants. This indicates differences in their systems installation, operation and maintenance. The performance indicator developed to evaluate the performance indices for the selected stations can also be applicable to other power stations in Nigeria and elsewhere. Measures to improve the performance indices of the plants have been suggested in this paper